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AB248596

Anti-LPP antibody [EPR6479(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal LPP antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Lipoma-preferred partner, LIM domain-containing preferred translocation partner in lipoma, LPP

2 Images
Flow Cytometry (Intracellular) - Anti-LPP antibody [EPR6479(2)] - BSA and Azide free (AB248596)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-LPP antibody [EPR6479(2)] - BSA and Azide free (AB248596)

This data was developed using ab133651, the same antibody clone in a different buffer formulation.

Intracellular flow cytometrical analysis of LPP in permeabilized HeLa cells labelled with ab133651 at a 1/10 dilution (red), or with a Rabbit IgG control (negative) (green).

Western blot - Anti-LPP antibody [EPR6479(2)] - BSA and Azide free (AB248596)
  • WB

Unknown

Western blot - Anti-LPP antibody [EPR6479(2)] - BSA and Azide free (AB248596)

This data was developed using ab133651, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LPP antibody [EPR6479(2)] (<a href='/en-us/products/primary-antibodies/lpp-antibody-epr64792-ab133651'>ab133651</a>) at 1/1000 dilution

Lane 1:

Caco2 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 122 kDa,66 kDa

Observed band size: 190 kDa,75 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6479(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248596 is the carrier-free version of ab133651.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Also suggested to serve as a scaffold protein upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus.
See full target information LPP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com