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AB166784

Anti-LRAT antibody

3

(1 Review)

|

(2 Publications)

Rabbit Polyclonal LRAT antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LRAT.

View Alternative Names

Lecithin retinol acyltransferase, Phosphatidylcholine--retinol O-acyltransferase, LRAT

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRAT antibody (AB166784)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRAT antibody (AB166784)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling LRAT with ab166784 at 5 µg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-LRAT antibody (AB166784)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LRAT antibody (AB166784)

Immunofluorescent analysis of HUVEC cells labeling LRAT with ab166784 at 1/100 dilution in the presence (right panel) or absence (left panel) of immunizing peptide.

Western blot - Anti-LRAT antibody (AB166784)
  • WB

Unknown

Western blot - Anti-LRAT antibody (AB166784)

All lanes:

Western blot - Anti-LRAT antibody (ab166784) at 1/500 dilution

Lane 1:

COLO205 cell extract

Lane 2:

COLO205 cell extract with immunizing peptide

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human LRAT. The exact immunogen used to generate this antibody is proprietary information.

O95237

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters (PubMed : 9920938). Retinyl esters are storage forms of vitamin A (Probable). LRAT plays a critical role in vision (Probable). It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments (Probable). Required for the survival of cone photoreceptors and correct rod photoreceptor cell morphology (By similarity).
See full target information LRAT
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BioFactors (Oxford, England) 46:788-802 PubMed32856377

2020

Inhibition of microRNA-27b-3p relieves osteoarthritis pain via regulation of KDM4B-dependent DLX5.

Applications

Unspecified application

Species

Unspecified reactive species

Guixiang Zhang,Yang Zhou,Manman Su,Xucheng Yang,Biyun Zeng

Frontiers in bioengineering and biotechnology 8:456 PubMed32500067

2020

Optimisation of a Novel Bio-Substrate as a Treatment for Atrophic Age-Related Macular Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel McCormick,Ian Pearce,Stephen Kaye,Atikah Haneef
View all publications
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Product promise

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