Anti-LRG1/LRG antibody [EPR12362]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (AB178698)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling LRG1/LRG with purified ab178698 at 1/200 dilution (0.57 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRG1/LRG antibody [EPR12362] (AB178698)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LRG1/LRG with unpurified ab178698 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-LRG1/LRG antibody [EPR12362] (AB178698)

All lanes:

Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human serum lysate at 10 µg

Predicted band size: 38 kDa

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• WB

Lab

Western blot - Anti-LRG1/LRG antibody [EPR12362] (AB178698)

The band observed is consistent with what has been described in PMID : 28081565

All lanes:

Western blot - Anti-LRG1/LRG antibody [EPR12362] (ab178698) at 1/10000 dilution

All lanes:

Human plasma lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 38 kDa

Observed band size: 50 kDa

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• WB

CiteAb

Western blot - Anti-LRG1/LRG antibody [EPR12362] (AB178698)

LRG1/LRG western blot using anti-LRG1/LRG antibody [EPR12362] ab178698. Publication image and figure legend from Hong, Q., Wang, S., et al., 2020, Biomed Res Int, PubMed 32337221.

ab178698 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab178698 please see the product overview.

Expression and methylation level in carcinoma and paracarcinoma samples of ccRCC. (a) mRNA expression of LRG1 in carcinoma and paracarcinoma tissues (p < 0.01, n = 6) detected by qPCR. (b, c) Protein expression of LRG1 in carcinoma and paracarcinoma tissues (p < 0.01, n = 6) detected by western blot. (d) LRG1 methylation level of CpG1 and CpG2 is downregulated in ccRCC tissue (p < 0.0001).

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