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AB202418

Anti-LRMP antibody [EPR17852]

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(3 Publications)

Rabbit Recombinant Monoclonal LRMP antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

JAW1, LRMP, IRAG2, Lymphoid-restricted membrane protein, Protein Jaw1

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-LRMP antibody [EPR17852] (AB202418)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LRMP antibody [EPR17852] (AB202418)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized Ramos (Human Burkitt's lymphoma cell line) cells labeling LRMP with ab202418 at 1/300 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on Ramos cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202418 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRMP antibody [EPR17852] (AB202418)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRMP antibody [EPR17852] (AB202418)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling LRMP with ab202418 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human tonail tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-LRMP antibody [EPR17852] (AB202418)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-LRMP antibody [EPR17852] (AB202418)

Intracellular Flow Cytometry analysis of Jurkat cells labelling LRMP (red) with purified ab202418 at dilution of 1/600. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-LRMP antibody [EPR17852] (AB202418)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LRMP antibody [EPR17852] (AB202418)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling LRMP with ab202418 at 1/300 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202418 at 1/300 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Western blot - Anti-LRMP antibody [EPR17852] (AB202418)
  • WB

Supplier Data

Western blot - Anti-LRMP antibody [EPR17852] (AB202418)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LRMP antibody [EPR17852] (ab202418) at 1/10000 dilution

Lane 1:

Human tonsil lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

Human fetal heart tissue lysate at 10 µg

Lane 4:

Human fetal kidney tissue lysate at 10 µg

Lane 5:

Human fetal spleen tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Exposure time: 3min

Western blot - Anti-LRMP antibody [EPR17852] (AB202418)
  • WB

Supplier Data

Western blot - Anti-LRMP antibody [EPR17852] (AB202418)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LRMP antibody [EPR17852] (ab202418) at 1/10000 dilution

Lane 1:

Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

NAMALWA (Human Burkitt's lymphoma) whole cell lysate at 20 µg

Lane 3:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 62 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-LRMP antibody [EPR17852] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17852

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/300", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/600", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the delivery of peptides to major histocompatibility complex (MHC) class I molecules; this occurs in a transporter associated with antigen processing (TAP)-independent manner. May play a role in taste signal transduction via ITPR3. May play a role during fertilization in pronucleus congression and fusion. Plays a role in maintaining nuclear shape, maybe as a component of the LINC complex and through interaction with microtubules. Plays a role in the regulation of cellular excitability by regulating the hyperpolarization-activated cyclic nucleotide-gated HCN4 channel activity (By similarity).
See full target information IRAG2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Discovery immunology 2:kyad027 PubMed38567068

2024

The glucocorticoid dexamethasone inhibits HIF-1α stabilization and metabolic reprogramming in lipopolysaccharide-stimulated primary macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Sally A Clayton,Chloe Lockwood,John D O'Neil,Kalbinder K Daley,Sofia Hain,Dina Abdelmottaleb,Oliwia O Bolimowska,Daniel A Tennant,Andrew R Clark

Frontiers in molecular biosciences 8:711928 PubMed34901148

2021

LRMP Associates With Immune Infiltrates and Acts as a Prognostic Biomarker in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Jin,Liwei Chen,Ning Zhou,Hong Ni,Lingling Zu,Jinling He,Lingqi Yang,Yifan Zhu,Xiaoyue Sun,Xiaojiang Li,Song Xu

Proceedings of the National Academy of Sciences of the United States of America 117:6092-6102 PubMed32127472

2020

Regulation of B cell receptor-dependent NF-κB signaling by the tumor suppressor KLHL14.

Applications

Unspecified application

Species

Unspecified reactive species

Jaewoo Choi,James D Phelan,George W Wright,Björn Häupl,Da Wei Huang,Arthur L Shaffer,Ryan M Young,Zhuo Wang,Hong Zhao,Xin Yu,Thomas Oellerich,Louis M Staudt
View all publications
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