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Rabbit Recombinant Multiclonal Lrp2 / Megalin antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt, IP and reacts with Human samples.

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Images

Flow Cytometry - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (AB317334), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (AB317334), expandable thumbnail
  • Immunoprecipitation - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (AB317334), expandable thumbnail
  • Western blot - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (AB317334), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (AB317334), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow CytIP
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Multiligand endocytic receptor (By similarity). Acts together with CUBN to mediate endocytosis of high-density lipoproteins (By similarity). Mediates receptor-mediated uptake of polybasic drugs such as aprotinin, aminoglycosides and polymyxin B (By similarity). In the kidney, mediates the tubular uptake and clearance of leptin (By similarity). Also mediates transport of leptin across the blood-brain barrier through endocytosis at the choroid plexus epithelium (By similarity). Endocytosis of leptin in neuronal cells is required for hypothalamic leptin signaling and leptin-mediated regulation of feeding and body weight (By similarity). Mediates endocytosis and subsequent lysosomal degradation of CST3 in kidney proximal tubule cells (By similarity). Mediates renal uptake of 25-hydroxyvitamin D3 in complex with the vitamin D3 transporter GC/DBP (By similarity). Mediates renal uptake of metallothionein-bound heavy metals (PubMed:15126248). Together with CUBN, mediates renal reabsorption of myoglobin (By similarity). Mediates renal uptake and subsequent lysosomal degradation of APOM (By similarity). Plays a role in kidney selenium homeostasis by mediating renal endocytosis of selenoprotein SEPP1 (By similarity). Mediates renal uptake of the antiapoptotic protein BIRC5/survivin which may be important for functional integrity of the kidney (PubMed:23825075). Mediates renal uptake of matrix metalloproteinase MMP2 in complex with metalloproteinase inhibitor TIMP1 (By similarity). Mediates endocytosis of Sonic hedgehog protein N-product (ShhN), the active product of SHH (By similarity). Also mediates ShhN transcytosis (By similarity). In the embryonic neuroepithelium, mediates endocytic uptake and degradation of BMP4, is required for correct SHH localization in the ventral neural tube and plays a role in patterning of the ventral telencephalon (By similarity). Required at the onset of neurulation to sequester SHH on the apical surface of neuroepithelial cells of the rostral diencephalon ventral midline and to control PTCH1-dependent uptake and intracellular trafficking of SHH (By similarity). During neurulation, required in neuroepithelial cells for uptake of folate bound to the folate receptor FOLR1 which is necessary for neural tube closure (By similarity). In the adult brain, negatively regulates BMP signaling in the subependymal zone which enables neurogenesis to proceed (By similarity). In astrocytes, mediates endocytosis of ALB which is required for the synthesis of the neurotrophic factor oleic acid (By similarity). Involved in neurite branching (By similarity). During optic nerve development, required for SHH-mediated migration and proliferation of oligodendrocyte precursor cells (By similarity). Mediates endocytic uptake and clearance of SHH in the retinal margin which protects retinal progenitor cells from mitogenic stimuli and keeps them quiescent (By similarity). Plays a role in reproductive organ development by mediating uptake in reproductive tissues of androgen and estrogen bound to the sex hormone binding protein SHBG (By similarity). Mediates endocytosis of angiotensin-2 (By similarity). Also mediates endocytosis of angiotensis 1-7 (By similarity). Binds to the complex composed of beta-amyloid protein 40 and CLU/APOJ and mediates its endocytosis and lysosomal degradation (By similarity). Required for embryonic heart development (By similarity). Required for normal hearing, possibly through interaction with estrogen in the inner ear (By similarity).

Alternative names

Low-density lipoprotein receptor-related protein 2, LRP-2, Glycoprotein 330, Megalin, gp330, LRP2

Recommended products

Rabbit Recombinant Multiclonal Lrp2 / Megalin antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt, IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
RM1150
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab317334 is the carrier-free version of Anti-Lrp2 / Megalin antibody [RM1150] ab317333.

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Flow Cytometry - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334), expandable thumbnail

    Flow Cytometry - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334)

    This data was developed using Anti-Lrp2 / Megalin antibody [RM1150] ab317333, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / JEG-3 (human placenta epithelial cell, Right) cells labelling Lrp2 / Megalin with Anti-Lrp2 / Megalin antibody [RM1150] ab317333 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: HeLa.

    Gated on viable cell.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334)

    This data was developed using Anti-Lrp2 / Megalin antibody [RM1150] ab317333, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized JEG-3 (human placenta epithelial cell) cells labelling Lrp2 / Megalin with Anti-Lrp2 / Megalin antibody [RM1150] ab317333 at 1/50 (10.0 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in JEG-3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Negative control: HeLa (PMID: 36064583).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunoprecipitation - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334), expandable thumbnail

    Immunoprecipitation - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334)

    This data was developed using Anti-Lrp2 / Megalin antibody [RM1150] ab317333, the same antibody clone in a different buffer formulation.

    Lrp2 / Megalin was immunoprecipitated from 0.35 mg JEG-3 (Human placenta epithelial choriocarcinoma) whole cell lysate with Anti-Lrp2 / Megalin antibody [RM1150] ab317333 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Lrp2 / Megalin antibody [RM1150] ab317333 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: JEG-3 (Human placenta epithelial choriocarcinoma) whole cell lysate

    Lane 2: Anti-Lrp2 / Megalin antibody [RM1150] ab317333 IP in JEG-3 (Human placenta epithelial choriocarcinoma) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Lrp2 / Megalin antibody [RM1150] ab317333 in JEG-3 whole cell lysate

    All lanes: Immunoprecipitation - Anti-Lrp2 / Megalin antibody [RM1150] (Anti-Lrp2 / Megalin antibody [RM1150] ab317333) at 1/30 dilution

    All lanes: JEG-3 (Human placenta epithelial choriocarcinoma) whole cell lysate with NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 8s

  • Western blot - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334), expandable thumbnail

    Western blot - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334)

    This data was developed using Anti-Lrp2 / Megalin antibody [RM1150] ab317333, the same antibody clone in a different buffer formulation.

    Negative control: HeLa, Raji (PMID: 35839778), human skeletal muscle.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    Exposure time: Lanes 1-4, 6-7: 10 seconds; Lanes 5: 1 second;

    All lanes: Western blot - Anti-Lrp2 / Megalin antibody [RM1150] (Anti-Lrp2 / Megalin antibody [RM1150] ab317333) at 1/1000 dilution

    Lane 1: JEG-3 (human placenta epithelial cell) whole cell lysate at 40 µg with NFDM/TBST

    Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 40 µg with NFDM/TBST

    Lane 3: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 40 µg with NFDM/TBST

    Lane 4: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 40 µg with NFDM/TBST

    Lane 5: Human kidney tissue lysate at 40 µg with NFDM/TBST

    Lane 6: Human placenta tissue lysate at 40 µg with NFDM/TBST

    Lane 7: Human skeletal muscle tissue lysate at 40 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 522 kDa, 124 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334)

    This data was developed using Anti-Lrp2 / Megalin antibody [RM1150] ab317333, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Lrp2 / Megalin with Anti-Lrp2 / Megalin antibody [RM1150] ab317333 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human skeletal muscle. The section was incubated with Anti-Lrp2 / Megalin antibody [RM1150] ab317333 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334)

    This data was developed using Anti-Lrp2 / Megalin antibody [RM1150] ab317333, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human clear cell renal cell carcinoma tissue labeling Lrp2 / Megalin with Anti-Lrp2 / Megalin antibody [RM1150] ab317333 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human clear cell renal cell carcinoma. The section was incubated with Anti-Lrp2 / Megalin antibody [RM1150] ab317333 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334)

    This data was developed using Anti-Lrp2 / Megalin antibody [RM1150] ab317333, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Lrp2 / Megalin with Anti-Lrp2 / Megalin antibody [RM1150] ab317333 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human placenta. The section was incubated with Anti-Lrp2 / Megalin antibody [RM1150] ab317333 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lrp2 / Megalin antibody [RM1150] - BSA and Azide free (ab317334)

    This data was developed using Anti-Lrp2 / Megalin antibody [RM1150] ab317333, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Lrp2 / Megalin with Anti-Lrp2 / Megalin antibody [RM1150] ab317333 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human kidney. The section was incubated with Anti-Lrp2 / Megalin antibody [RM1150] ab317333 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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