AB324976

Anti-LRP4 antibody [EPR30692-584]

RabMAb
Recombinant
20ul selling size
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Rabbit Recombinant Monoclonal LRP4 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Kiaa0816, Lrp4, Low-density lipoprotein receptor-related protein 4, LRP-4, LDLR dan, KIAA0816, LRP10, MEGF7, LRP4, Low-density lipoprotein receptor-related protein 4, LRP-4, Multiple epidermal growth factor-like domains 7, Megf7, Low-density lipoprotein receptor-related protein 4, LRP-4, Multiple epidermal growth factor-like domains 7

3 Images

Supplier Data

Western blot - Anti-LRP4 antibody [EPR30692-584] (AB324976)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : RAW 264.7, Beta-TC-6, Raji, HDLM-2

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 19936252; PMID : 26847765; PMID : 17119023).

Lanes 1-4 of this blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LRP4 antibody [EPR30692-584] (ab324976) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 48 µg

Lane 2:

MEF (mouse embryo fibroblast) whole cell lysate at 48 µg

Lane 3:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 48 µg

Lane 4:

Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 48 µg

Lane 5:

SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 48 µg

Lane 6:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 48 µg

Lane 7:

NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate at 48 µg

Lane 8:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 48 µg

Lane 9:

HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 270 kDa,124 kDa

true

Exposure time: 180s

Supplier Data

Western blot - Anti-LRP4 antibody [EPR30692-584] (AB324976)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : colon(adult), testis(adult), skeletal muscle(adult).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 19936252; PMID : 26847765; PMID : 17119023).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LRP4 antibody [EPR30692-584] (ab324976) at 1/1000 dilution

Lane 1:

Mouse E18 brain tissue lysate at 20 µg

Lane 2:

Mouse E18 skeletal muscle tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 5:

Mouse colon tissue lysate at 20 µg

Lane 6:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 270 kDa,124 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-LRP4 antibody [EPR30692-584] (AB324976)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : colon(adult), skeletal muscle(adult).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 19936252; PMID : 26847765; PMID : 17119023).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LRP4 antibody [EPR30692-584] (ab324976) at 1/1000 dilution

Lane 1:

Rat E15 brain tissue lysate at 48 µg

Lane 2:

Rat fetal brain tissue lysate at 48 µg

Lane 3:

Rat lung tissue lysate at 48 µg

Lane 4:

Rat skeletal muscle tissue lysate at 48 µg

Lane 5:

Rat colon tissue lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 270 kDa,124 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30692-584

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Plays an essential role in the process of digit differentiation (PubMed : 16517118).

See full target information Lrp4

Additional targets

,LRP4

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com