Rabbit Recombinant Monoclonal LRP5 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat, Transfected cell line - Human, Recombinant fragment - Mouse samples.
Images
![Western blot - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (AB256528), expandable thumbnail](https://content.abcam.com/products/images/lrp5-antibody-epr22477-218-bsa-and-azide-free-ab256528--western-blot-img3224.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (AB256528)")
![Immunoprecipitation - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (AB256528), expandable thumbnail](https://content.abcam.com/products/images/lrp5-antibody-epr22477-218-bsa-and-azide-free-ab256528--immunoprecipitation-img11854.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (AB256528)")
![Western blot - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (AB256528), expandable thumbnail](https://content.abcam.com/products/images/lrp5-antibody-epr22477-218-bsa-and-azide-free-ab256528--western-blot-img409941.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (AB256528)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Expected | Not recommended | Tested | Not recommended | Not recommended |
| Recombinant fragment - Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Transfected cell line - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human, Recombinant fragment - Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell line - Human, Recombinant fragment - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human, Mouse, Recombinant fragment - Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell line - Human, Recombinant fragment - Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell line - Human, Recombinant fragment - Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Acts as a coreceptor with members of the frizzled family of seven-transmembrane spanning receptors to transduce signal by Wnt proteins (PubMed:11336703, PubMed:11448771, PubMed:11719191, PubMed:15778503, PubMed:15908424, PubMed:16252235). Activates the canonical Wnt signaling pathway that controls cell fate determination and self-renewal during embryonic development and adult tissue regeneration (PubMed:11336703, PubMed:11719191). In particular, may play an important role in the development of the posterior patterning of the epiblast during gastrulation (By similarity). During bone development, regulates osteoblast proliferation and differentiation thus determining bone mass (PubMed:11719191). Mechanistically, the formation of the signaling complex between Wnt ligand, frizzled receptor and LRP5 coreceptor promotes the recruitment of AXIN1 to LRP5, stabilizing beta-catenin/CTNNB1 and activating TCF/LEF-mediated transcriptional programs (PubMed:11336703, PubMed:14731402, PubMed:24706814, PubMed:25920554). Acts as a coreceptor for non-Wnt proteins, such as norrin/NDP. Binding of norrin/NDP to frizzled 4/FZD4-LRP5 receptor complex triggers beta-catenin/CTNNB1-dependent signaling known to be required for retinal vascular development (PubMed:16252235, PubMed:27228167). Plays a role in controlling postnatal vascular regression in retina via macrophage-induced endothelial cell apoptosis (By similarity).
Alternative names
LR3, LRP7, LRP5, Low-density lipoprotein receptor-related protein 5, LRP-5, Low-density lipoprotein receptor-related protein 7, LRP-7
Recommended products
Rabbit Recombinant Monoclonal LRP5 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human, Rat, Transfected cell line - Human, Recombinant fragment - Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22477-218
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab256528 is the carrier-free version of Anti-LRP5 antibody [EPR22477-218] ab223203.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528), expandable thumbnail](https://content.abcam.com/products/images/lrp5-antibody-epr22477-218-bsa-and-azide-free-ab256528--western-blot-img3224.jpg "Western blot - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)")
Western blot - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)
This data was developed using the same antibody clone in a different buffer formulation (Anti-LRP5 antibody [EPR22477-218] ab223203).
Lanes 1-3: Merged signal (red and green). Green - Anti-LRP5 antibody [EPR22477-218] ab223203 observed at 180-200 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.
Anti-LRP5 antibody [EPR22477-218] ab223203 Anti-LRP5 antibody [EPR22477-218] was shown to specifically react with LRP5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human LRP5 knockout HEK-293T cell line ab266618 (knockout cell lysate Human LRP5 knockout HEK-293T cell lysate ab257202) was used. Wild-type and LRP5 knockout samples were subjected to SDS-PAGE. Anti-LRP5 antibody [EPR22477-218] ab223203 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-LRP5 antibody [EPR22477-218] (Anti-LRP5 antibody [EPR22477-218] ab223203) at 1/500 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: LRP5 knockout HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human LRP5 knockout HEK-293T cell line (Human LRP5 knockout HEK-293T cell line ab266618)
Lane 3: SW620 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 179 kDa
Observed band size: 180-200 kDa
![Immunoprecipitation - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528), expandable thumbnail](https://content.abcam.com/products/images/lrp5-antibody-epr22477-218-bsa-and-azide-free-ab256528--immunoprecipitation-img11854.jpg "Immunoprecipitation - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)")
Immunoprecipitation - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)
LRP5 was immunoprecipitated from 0.35 mg 3T3-L1 (mouse embryonic fibroblast) differentiated adipocytes whole cell lysate 10μg with Anti-LRP5 antibody [EPR22477-218] ab223203 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-LRP5 antibody [EPR22477-218] ab223203. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Lane 1: 3T3-L1 (mouse embryonic fibroblast) differentiated adipocytes whole cell lysate 10μg.
Lane 2: Anti-LRP5 antibody [EPR22477-218] ab223203 IP in 3T3-L1 differentiated adipocytes whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-LRP5 antibody [EPR22477-218] ab223203 in 3T3-L1 differentiated adipocytes whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 mins.
Differentiation procedure: https://www.abcam.com/protocols/differentiation-of-3t3-l1-cells-into-adipocyte-like-cells-protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LRP5 antibody [EPR22477-218] ab223203).
All lanes: Immunoprecipitation - Anti-LRP5 antibody [EPR22477-218] (Anti-LRP5 antibody [EPR22477-218] ab223203)
Predicted band size: 179 kDa
Observed band size: 180-200 kDa
![Western blot - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528), expandable thumbnail](https://content.abcam.com/products/images/lrp5-antibody-epr22477-218-bsa-and-azide-free-ab256528--western-blot-img409941.jpg "Western blot - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)")
Western blot - Anti-LRP5 antibody [EPR22477-218] - BSA and Azide free (ab256528)
Blocking and dilution buffer: 5%NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LRP5 antibody [EPR22477-218] ab223203).Lanes 1 - 3: Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] ab76417) at 1/10000 dilution
Lanes 4 - 6: Western blot - Anti-LRP5 antibody [EPR22477-218] (Anti-LRP5 antibody [EPR22477-218] ab223203) at 1/10000 dilution
Lanes 1 and 4: 293T transfected with blank vector whole cell lysate at 20 µg
Lanes 2 and 5: 293T transfected with LRP6 overexpression vector whole cell lysate at 20 µg
Lanes 3 and 6: 293T transfected with LRP5 overexpression vector whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 179 kDa
Observed band size: 180 kDa
Exposure time: 180s
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