AB134146

Anti-LRP6 antibody [EPR2423(2)]

Name: Anti-LRP6 antibody [EPR2423(2)] (ab134146) | Abcam
Brand: Abcam Limited
SKU: AB134146
Price: 365 USD
Availability: InStock
Rating: 4 (5 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(5 Reviews)

(40 Publications)

Rabbit Recombinant Monoclonal LRP6 antibody. Suitable for WB and reacts with Human samples. Cited in 40 publications.

View Alternative Names

Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6

2 Images

Lab

Western blot - Anti-LRP6 antibody [EPR2423(2)] (AB134146)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : LRP6 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab134146 observed at 220 kDa. Red - loading control, ab18058, observed at 124 kDa.

ab134146 was shown to recognize LRP6 when LRP6 knockout samples were used, along with additional cross-reactive bands. Wild-type and LRP6 knockout samples were subjected to SDS-PAGE. ab134146 and ab18058 (loading control to Vinculin) were diluted 1/10000 and 1/1000 respectively, and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146)

Predicted band size: 180 kDa

Observed band size: 220 kDa

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Western blot - Anti-LRP6 antibody [EPR2423(2)] (AB134146)

All lanes:

Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146) at 1/1000 dilution

Lane 1:

HeLa cell lysates at 10 µg

Lane 2:

HepG2 cell lysates at 10 µg

Lane 3:

293T cell lysates at 10 µg

Lane 4:

Jurkat cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 180 kDa

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Carrier free

Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2423(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalosomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity).

See full target information LRP6

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed39037913

2024

Liver microRNA transcriptome reveals miR-182 as link between type 2 diabetes and fatty liver disease in obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Christin Krause,Jan H Britsemmer,Miriam Bernecker,Anna Molenaar,Natalie Taege,Nuria Lopez-Alcantara,Cathleen Geißler,Meike Kaehler,Katharina Iben,Anna Judycka,Jonas Wagner,Stefan Wolter,Oliver Mann,Paul Pfluger,Ingolf Cascorbi,Hendrik Lehnert,Kerstin Stemmer,Sonja C Schriever,Henriette Kirchner

Neuroscience applied 3:104070 PubMed40656072

2024

An investigation on the alterations in Wnt signaling in ADHD across developmental stages.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie Monet Walter,Cristine Marie Yde Ohki,Michelle Rickli,Lukasz Smigielski,Susanne Walitza,Edna Grünblatt

Experimental hematology & oncology 13:27 PubMed38438856

2024

The multi-CDK inhibitor dinaciclib reverses bromo- and extra-terminal domain (BET) inhibitor resistance in acute myeloid leukemia via inhibition of Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander R Marr,Madeline Halpin,Dominique L Corbin,Yerdanos Asemelash,Steven Sher,Britten K Gordon,Ethan C Whipp,Shaneice Mitchell,Bonnie K Harrington,Shelley Orwick,Samon Benrashid,Virginia M Goettl,Vedat Yildiz,Andrew D Mitchell,Olivia Cahn,Alice S Mims,Karilyn T M Larkin,Meixao Long,James Blachly,Jennifer A Woyach,Rosa Lapalombella,Nicole R Grieselhuber

Respiratory research 24:227 PubMed37741976

2023

CTGF promotes the repair and regeneration of alveoli after acute lung injury by promoting the proliferation of subpopulation of AEC2s.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhui Sun,Huacai Zhang,Di Liu,Wenyi Liu,Juan Du,Dalin Wen,Luoxi Li,Anqiang Zhang,Jianxin Jiang,Ling Zeng

Nature communications 14:2861 PubMed37208334

2023

ADAR1-mediated RNA editing of SCD1 drives drug resistance and self-renewal in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tin-Lok Wong,Jia-Jian Loh,Shixun Lu,Helen H N Yan,Hoi Cheong Siu,Ren Xi,Dessy Chan,Max J F Kam,Lei Zhou,Man Tong,John A Copland,Leilei Chen,Jing-Ping Yun,Suet Yi Leung,Stephanie Ma

Frontiers in cell and developmental biology 11:1173688 PubMed37091972

2023

Balancing WNT signalling in early forebrain development: The role of LRP4 as a modulator of LRP6 function.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Geng,Fabian Paul,Izabela Kowalczyk,Sandra Raimundo,Anje Sporbert,Tamrat Meshka Mamo,Annette Hammes

Journal of cellular and molecular medicine 27:950-961 PubMed36892460

2023

Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β-catenin signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wang,Ying Gan,Yuan Dong,Jie Zhou,Endong Zhu,Hairui Yuan,Xiaoxia Li,Baoli Wang

Science advances 9:eabo7421 PubMed36638182

2023

A genetic variant of the Wnt receptor LRP6 accelerates synapse degeneration during aging and in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Megan E Jones,Johanna Büchler,Tom Dufor,Ernest Palomer,Samuel Teo,Nuria Martin-Flores,Katharina Boroviak,Emmanouil Metzakopian,Alasdair Gibb,Patricia C Salinas

Metabolites 12: PubMed35323704

2022

Whole Exome/Genome Sequencing Joint Analysis of a Family with Oligogenic Familial Hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Youmna Ghaleb,Sandy Elbitar,Anne Philippi,Petra El Khoury,Yara Azar,Miangaly Andrianirina,Alexia Loste,Yara Abou-Khalil,Gaël Nicolas,Marie Le Borgne,Philippe Moulin,Mathilde Di-Filippo,Sybil Charrière,Michel Farnier,Cécile Yelnick,Valérie Carreau,Jean Ferrières,Jean-Michel Lecerf,Alexa Derksen,Geneviève Bernard,Marie-Soleil Gauthier,Benoit Coulombe,Dieter Lütjohann,Bertrand Fin,Anne Boland,Robert Olaso,Jean-François Deleuze,Jean-Pierre Rabès,Catherine Boileau,Marianne Abifadel,Mathilde Varret

Stem cell research & therapy 13:53 PubMed35120575

2022

N-myc downstream regulated gene 1 suppresses osteoblast differentiation through inactivating Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Shi,Yunzhu Cen,Liying Shan,Lijie Tian,Endong Zhu,Hairui Yuan,Xiaoxia Li,Ying Liu,Baoli Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LRP6 antibody [EPR2423(2)]

Western blot - Anti-LRP6 antibody [EPR2423(2)] (AB134146)

Western blot - Anti-LRP6 antibody [EPR2423(2)] (AB134146)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (40)

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