Rabbit Recombinant Monoclonal LRP6 antibody. Suitable for WB and reacts with Human samples. Cited in 31 publications.
Images
![Western blot - Anti-LRP6 antibody [EPR2423(2)] (AB134146), expandable thumbnail](https://content.abcam.com/products/images/lrp6-antibody-epr24232-ab134146--western-blot-img57995.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LRP6 antibody [EPR2423(2)] (AB134146)")
![Western blot - Anti-LRP6 antibody [EPR2423(2)] (AB134146), expandable thumbnail](https://content.abcam.com/products/images/lrp6-antibody-epr24232-ab134146--western-blot-img30813.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LRP6 antibody [EPR2423(2)] (AB134146)")
Publications
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- Nature communications 14:28612023ADAR1-mediated RNA editing of SCD1 drives drug resistance and self-renewal in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTin-Lok Wong,Jia-Jian Loh,Shixun Lu,Helen H N Yan,Hoi Cheong Siu,Ren Xi,Dessy Chan,Max J F Kam,Lei Zhou,Man Tong,John A Copland,Leilei Chen,Jing-Ping Yun,Suet Yi Leung,Stephanie MaPubMed 37208334
- Journal of cellular and molecular medicine 27:950-9612023Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β-catenin signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Wang,Ying Gan,Yuan Dong,Jie Zhou,Endong Zhu,Hairui Yuan,Xiaoxia Li,Baoli WangPubMed 36892460
- Metabolites 12:2022Whole Exome/Genome Sequencing Joint Analysis of a Family with Oligogenic Familial Hypercholesterolemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoumna Ghaleb,Sandy Elbitar,Anne Philippi,Petra El Khoury,Yara Azar,Miangaly Andrianirina,Alexia Loste,Yara Abou-Khalil,Gaël Nicolas,Marie Le Borgne,Philippe Moulin,Mathilde Di-Filippo,Sybil Charrière,Michel Farnier,Cécile Yelnick,Valérie Carreau,Jean Ferrières,Jean-Michel Lecerf,Alexa Derksen,Geneviève Bernard,Marie-Soleil Gauthier,Benoit Coulombe,Dieter Lütjohann,Bertrand Fin,Anne Boland,Robert Olaso,Jean-François Deleuze,Jean-Pierre Rabès,Catherine Boileau,Marianne Abifadel,Mathilde VarretPubMed 35323704
- Stem cell research & therapy 13:532022N-myc downstream regulated gene 1 suppresses osteoblast differentiation through inactivating Wnt/β-catenin signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoli Shi,Yunzhu Cen,Liying Shan,Lijie Tian,Endong Zhu,Hairui Yuan,Xiaoxia Li,Ying Liu,Baoli WangPubMed 35120575
- Cellular and molecular life sciences : CMLS 79:1072022Metastasis suppressor 1 controls osteoblast differentiation and bone homeostasis through regulating Src-Wnt/β-catenin signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Chen,Liying Shan,Ying Gan,Lijie Tian,Jie Zhou,Endong Zhu,Hairui Yuan,Xiaoxia Li,Baoli WangPubMed 35094173
- Chinese medicine 17:112022Astragalus membranaceus (Huangqi) and Rhizoma curcumae (Ezhu) decoction suppresses colorectal cancer via downregulation of Wnt5/β-Catenin signal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Bian,Gang Wang,Jing Zhou,Gang Yin,Tiantian Liu,Li Liang,Xinyue Yang,Wen Zhang,Kexin Ni,Decai Tang,Yun YuPubMed 34991661
- The journal of gene medicine 24:e33432021CDK14/β-catenin/TCF4/miR-26b positive feedback regulation modulating pancreatic cancer cell phenotypes in vitro and tumor growth in mice model in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunpeng Sun,Pengfei Wang,Qiyu Zhang,Huanhuan WuPubMed 33871149
- Frontiers in oncology 10:6116992021Hypoxia-Induced miR-137 Inhibition Increased Glioblastoma Multiforme Growth and Chemoresistance Through LRP6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong-Mei Li,Qiu-Dan Chen,Gui-Ning Wei,Jie Wei,Jian-Xing Yin,Jun-Hui He,Xin Ge,Zhu-Mei ShiPubMed 33718112
- The Journal of cell biology 220:2021Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergi Junyent,Joshua Reeves,Eileen Gentleman,Shukry J HabibPubMed 33606876
- Physiological reports 9:e146962021Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinhua Miao,Jiewu Huang,Congwei Luo,Huiyun Ye,Xian Ling,Qinyu Wu,Weiwei Shen,Lili ZhouPubMed 33463897
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalosomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity).
Alternative names
Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6
Recommended products
Rabbit Recombinant Monoclonal LRP6 antibody. Suitable for WB and reacts with Human samples. Cited in 31 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR2423(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146), expandable thumbnail](https://content.abcam.com/products/images/lrp6-antibody-epr24232-ab134146--western-blot-img57995.jpg "Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146)")
Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: LRP6 knockout HAP1 cell lysate (20 μg)
Lane 3: HeLa cell lysate (20 μg)
Lane 4: HepG2 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab134146 observed at 220 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab134146 was shown to recognize LRP6 when LRP6 knockout samples were used, along with additional cross-reactive bands. Wild-type and LRP6 knockout samples were subjected to SDS-PAGE. ab134146 and ab18058 (loading control to Vinculin) were diluted 1/10000 and 1/1000 respectively, and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146)
Predicted band size: 180 kDa
Observed band size: 220 kDa
![Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146), expandable thumbnail](https://content.abcam.com/products/images/lrp6-antibody-epr24232-ab134146--western-blot-img30813.jpg "Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146)")
Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146)
All lanes: Western blot - Anti-LRP6 antibody [EPR2423(2)] (ab134146) at 1/1000 dilution
Lane 1: HeLa cell lysates at 10 µg
Lane 2: HepG2 cell lysates at 10 µg
Lane 3: 293T cell lysates at 10 µg
Lane 4: Jurkat cell lysates at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 180 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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