Rabbit Recombinant Monoclonal LRP6 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free (AB232484), expandable thumbnail](https://content.abcam.com/products/images/lrp6-antibody-epr24232-bsa-and-azide-free-ab232484--western-blot-img57995.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free (AB232484)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalosomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity).
Alternative names
Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6
Recommended products
Rabbit Recombinant Monoclonal LRP6 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2423(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232484 is the carrier-free version of Anti-LRP6 antibody [EPR2423(2)] ab134146.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free (ab232484), expandable thumbnail](https://content.abcam.com/products/images/lrp6-antibody-epr24232-bsa-and-azide-free-ab232484--western-blot-img57995.jpg "Western blot - Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free (ab232484)")
Western blot - Anti-LRP6 antibody [EPR2423(2)] - BSA and Azide free (ab232484)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: LRP6 knockout HAP1 cell lysate (20 μg)
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate (20 μg)
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-LRP6 antibody [EPR2423(2)] ab134146 observed at 220 kDa. Red - loading control, ab18058, observed at 124 kDa.
Anti-LRP6 antibody [EPR2423(2)] ab134146 was shown to recognize LRP6 when LRP6 knockout samples were used, along with additional cross-reactive bands. Wild-type and LRP6 knockout samples were subjected to SDS-PAGE. Anti-LRP6 antibody [EPR2423(2)] ab134146 and ab18058 (loading control to Vinculin) were diluted 1/10000 and 1/1000 respectively, and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LRP6 antibody [EPR2423(2)] ab134146).
All lanes: Western blot - Anti-LRP6 antibody [EPR2423(2)] (Anti-LRP6 antibody [EPR2423(2)] ab134146)
Predicted band size: 180 kDa
Observed band size: 220 kDa
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