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AB76417

Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y]

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(4 Publications)

Rabbit Recombinant Monoclonal LRP6 phospho S1490 antibody. Suitable for WB and reacts with Human, Transfected cell line - Human samples. Cited in 4 publications.

View Alternative Names

Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6

2 Images
Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (AB76417)
  • WB

Unknown

Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (AB76417)

All lanes:

Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (ab76417) at 1/1000 dilution

Lane 1:

HeLa cell lysates, untreated at 10 µg

Lane 2:

HeLa cell lysates treated with Calyculin at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Observed band size: 210 kDa,33 kDa

false

Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (AB76417)
  • WB

Lab

Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (AB76417)

Blocking and dilution buffer : 5%NFDM/TBST.

Lanes 1 - 3:

Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (ab76417) at 1/10000 dilution

Lanes 4 - 6:

Western blot - Anti-LRP5 antibody [EPR22477-218] (<a href='/en-us/products/primary-antibodies/lrp5-antibody-epr22477-218-ab223203'>ab223203</a>) at 1/10000 dilution

Lanes 1 and 4:

293T transfected with blank vector whole cell lysate at 20 µg

Lanes 2 and 5:

293T transfected with LRP6 overexpression vector whole cell lysate at 20 µg

Lanes 3 and 6:

293T transfected with LRP5 overexpression vector whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 179 kDa

Observed band size: 180 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2360Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Transfected cell line - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalosomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity).
See full target information LRP6 phospho S1490

Additional targets

LRP5 phospho S1503
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The journal of gene medicine 24:e3343 PubMed33871149

2021

CDK14/β-catenin/TCF4/miR-26b positive feedback regulation modulating pancreatic cancer cell phenotypes in vitro and tumor growth in mice model in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yunpeng Sun,Pengfei Wang,Qiyu Zhang,Huanhuan Wu

Physiological reports 9:e14696 PubMed33463897

2021

Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Miao,Jiewu Huang,Congwei Luo,Huiyun Ye,Xian Ling,Qinyu Wu,Weiwei Shen,Lili Zhou

Journal of cellular biochemistry 120:3874-3886 PubMed30474270

2018

LncRNA H19/miR-194/PFTK1 axis modulates the cell proliferation and migration of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yunpeng Sun,Qiandong Zhu,Wenjun Yang,Yunfeng Shan,Zhengping Yu,Qiyu Zhang,Huanhuan Wu

Birth defects research 110:63-71 PubMed28960852

2017

Novel Mutation of LRP6 Identified in Chinese Han Population Links Canonical WNT Signaling to Neural Tube Defects.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwen Shi,Xueyan Yang,Bin-Bin Li,Shuxia Chen,Luming Yang,Liangping Cheng,Ting Zhang,Hongyan Wang,Yufang Zheng
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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