AB247469

Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal LRP6 phospho S1490 antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell line - Human samples.

View Alternative Names

Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6, LR3, LRP7, LRP5, Low-density lipoprotein receptor-related protein 5, LRP-5, Low-density lipoprotein receptor-related protein 7, LRP-7

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Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] - BSA and Azide free (AB247469)

This data was developed using ab76417, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] (<a href='/en-us/products/primary-antibodies/lrp6-phospho-s1490-lrp5-phospho-s1503-antibody-ep2360y-ab76417'>ab76417</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysates, untreated at 10 µg

Lane 2:

HeLa cell lysates treated with Calyculin at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Observed band size: 210 kDa,33 kDa

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Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] - BSA and Azide free (AB247469)

Blocking and dilution buffer : 5%NFDM/TBST. This data was developed using ab76417, the same antibody clone in a different buffer formulation.

Lanes 1 - 3:

Lanes 4 - 6:

Western blot - Anti-LRP5 antibody [EPR22477-218] (<a href='/en-us/products/primary-antibodies/lrp5-antibody-epr22477-218-ab223203'>ab223203</a>) at 1/10000 dilution

Lanes 1 and 4:

293T transfected with blank vector whole cell lysate at 20 µg

Lanes 2 and 5:

293T transfected with LRP6 overexpression vector whole cell lysate at 20 µg

Lanes 3 and 6:

293T transfected with LRP5 overexpression vector whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 179 kDa

Observed band size: 180 kDa

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Exposure time: 180s

Unconjugated

Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2360Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab247469 is the carrier-free version of ab76417.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes (PubMed : 11357136, PubMed : 11448771, PubMed : 15778503, PubMed : 16341017, PubMed : 16513652, PubMed : 17326769, PubMed : 17400545, PubMed : 19107203, PubMed : 19293931, PubMed : 19801552, PubMed : 28341812, PubMed : 34896607). Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in various processes including retinal angiogenesis and bone formation (PubMed : 11357136, PubMed : 11448771, PubMed : 15778503, PubMed : 16341017, PubMed : 16513652, PubMed : 17326769, PubMed : 17400545, PubMed : 19107203, PubMed : 19293931, PubMed : 19801552, PubMed : 28341812, PubMed : 34896607). The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalosomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin (PubMed : 16513652). Required for posterior patterning of the epiblast during gastrulation (By similarity).

See full target information LRP6 pS1490

Additional targets

LRP5 phospho S1503

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] - BSA and Azide free

Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] - BSA and Azide free (AB247469)

Western blot - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody [EP2360Y] - BSA and Azide free (AB247469)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Product promise