Rabbit Recombinant Monoclonal LRPAP1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Images
![Western blot - Anti-LRPAP1 antibody [EPR3327] (AB76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--western-blot-img121090.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LRPAP1 antibody [EPR3327] (AB76500)")
![Western blot - Anti-LRPAP1 antibody [EPR3327] (AB76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--western-blot-img126802.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LRPAP1 antibody [EPR3327] (AB76500)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (AB76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61708.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (AB76500)")
![Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (AB76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--immunoprecipitation-img108822.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (AB76500)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (AB76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77789.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (AB76500)")
Publications
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- Signal transduction and targeted therapy 8:3742023Secreted LRPAP1 binds and triggers IFNAR1 degradation to facilitate virus evasion from cellular innate immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuangcan Li,Xiong Wang,Yiran Wang,Yichen Li,Ying Chen,Yin-Ting Wong,Jufang He,Ming-Liang HePubMed 37743411
- The Journal of biological chemistry 297:1008422021Molecular chaperone RAP interacts with LRP1 in a dynamic bivalent mode and enhances folding of ligand-binding regions of other LDLR family receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEkaterina Marakasova,Philip Olivares,Elena Karnaukhova,Haarin Chun,Nancy E Hernandez,James H Kurasawa,Gabriela U Hassink,Svetlana A Shestopal,Dudley K Strickland,Andrey G SarafanovPubMed 34058195
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Not recommended |
| Mouse | Expected | Expected | Not recommended | Tested | Not recommended |
| Rat | Expected | Expected | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.
Alternative names
A2MRAP, LRPAP1, Alpha-2-macroglobulin receptor-associated protein, Alpha-2-MRAP, Low density lipoprotein receptor-related protein-associated protein 1, RAP
Recommended products
Rabbit Recombinant Monoclonal LRPAP1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3327
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--western-blot-img121090.jpg "Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500)")
Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST
All lanes: Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/6000 dilution
Lane 1: Human fetal kidney tissue lysate at 10 µg
Lane 2: Mouse kidney tissue lysate at 10 µg
Lane 3: Rat kidney tissue lysate at 10 µg
Lane 4: 293T cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 45 kDa
![Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--western-blot-img126802.jpg "Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500)")
Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST
All lanes: Western blot - Anti-LRPAP1 antibody [EPR3327] (ab76500) at 1/3000 dilution
Lane 1: Human fetal kidney tissue lysate at 10 µg
Lane 2: Mouse kidney tissue lysate at 10 µg
Lane 3: Rat kidney tissue lysate at 10 µg
Lane 4: 293T cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 45 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61708.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with unpurified ab76500 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
![Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--immunoprecipitation-img108822.jpg "Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)")
Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)
ab76500 (unpurified) at 1/300 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)
Predicted band size: 41 kDa
Observed band size: 45 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77789.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling LRPAP1 with purified ab76500 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
![Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--immunoprecipitation-img128920.jpg "Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)")
Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)
ab76500 (purified) at 1/150 immunoprecipitating LRPAP1 in human fetal kidney tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)
Predicted band size: 41 kDa
Observed band size: 45 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126136.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling LRPAP1 with unpurified ab76500 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--immunoprecipitation-img128920.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-LRPAP1 antibody [EPR3327] (ab76500)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500), expandable thumbnail](https://content.abcam.com/products/images/lrpap1-antibody-epr3327-ab76500--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126136.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3327] (ab76500)")
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