Rabbit Polyclonal LRPPRC/GP130 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LRPPRC aa 1300 to C-terminus.
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- Communications biology 5:2372022KPT330 improves Cas9 precision genome- and base-editing by selectively regulating mRNA nuclear export.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Ru Cui,Shao-Jie Wang,Tiancheng Ma,Peihong Yu,Jun Chen,Taijie Guo,Genyi Meng,Biao Jiang,Jiajia Dong,Jia LiuPubMed 35301428
- Frontiers in pharmacology 10:3972019lncRNA ZEB1-AS1 Mediates Oxidative Low-Density Lipoprotein-Mediated Endothelial Cells Injury by Post-transcriptional Stabilization of NOD2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohui Xu et. al.PubMed 31040785
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LRPPRC aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P42704
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. Positively modulates nuclear export of mRNAs containing the EIF4E sensitivity element (4ESE) by binding simultaneously to both EIF4E and the 4ESE and acting as a platform for assembly for the RNA export complex (PubMed:19262567, PubMed:28325843). Also binds to exportin XPO1/CRM1 to engage the nuclear pore and traffic the bound mRNAs to the cytoplasm (PubMed:28325843). May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA (By similarity). Required for maintaining mitochondrial potential (PubMed:23822101). Suppresses the initiation of basal levels of autophagy and mitophagy by sustaining BCL2 levels (PubMed:23822101).
Alternative names
LRP130, LRPPRC, 130 kDa leucine-rich protein, GP130, LRP 130
Recommended products
Rabbit Polyclonal LRPPRC/GP130 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LRPPRC aa 1300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LRPPRC aa 1300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P42704
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunoprecipitation - Anti-LRPPRC/GP130 antibody (ab205022)
Detection of human LRPPRC/GP130 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.
Lane 1: Rabbit polyclonal to LRPPRC/GP130 (ab205022) at 6 μg/mg lysate
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 30 seconds. Anti-FTSJD2 antibody ab70386 was used at 0.4 ug/mL for Western blot.
All lanes: Immunoprecipitation - Anti-LRPPRC/GP130 antibody (ab205022)
Predicted band size: 158 kDa
Western blot - Anti-LRPPRC/GP130 antibody (ab205022)
All lanes: Western blot - Anti-LRPPRC/GP130 antibody (ab205022) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 158 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPPRC/GP130 antibody (ab205022)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling LRPPRC/GP130 with ab205022 at a 1/1000 dilution.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-LRPPRC/GP130 antibody (ab205022)
LRPPRC/GP130 western blot using anti-LRPPRC/GP130 antibody ab205022. Publication image and figure legend from Xu, X., Ma, C., et al., 2019, Front Pharmacol, PubMed 31040785.
ab205022 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab205022 please see the product overview.
ZEB1-AS1 regulates mRNA stability of NOD2 via recruiting LRPPRC. (A) LRPPRC was silenced by specific siRNAs at both transcript (down panel) and protein (upper panel) levels, ∗∗P < 0.01 (Mann–Whitney U-test). (B) NOD2 expression was detected in HUVECs overexpressed with ZEB1-AS1 and/or silenced with LRPPRC, ∗P < 0.05 (Mann–Whitney U-test). (C) HUVECs lysates were incubated with in vitro synthesized, biotin-labeled control LacZ DNA probes or DNA probes against ZEB1-AS1 for the biotinylated oligonucleotide pulldown assay. The precipitates from the pulldown were analyzed by qRT-PCR to detect the interacting mRNAs. ∗∗P < 0.01 (Mann–Whitney U-test) compared to respective LacZ probes. (D) RIP was performed using anti-LRPPRC and control IgG antibodies, followed by qRT-PCR to examine the enrichment of NOD2 and U6. U6 served as negative controls. ∗∗P < 0.01 (Mann–Whitney U-test) compared to respective IgG controls. (E) HUVECs expressing si-ZEB1-AS1#2 or si-LRPPRC were treated with actinomycin D (5 μg/mL) for the indicated periods of time. ∗P < 0.05 (Mann–Whitney U-test) compared to si-NC group (F). HUVECs expressing si-NC, or p-ZEB1-AS1, or p-ZEB1-AS1 + si-LRPPRC were treated with actinomycin D (5 μg/mL) for the indicated periods of time. Total RNA was purified and then analyzed using qRT-PCR to examine the mRNA half-life of NOD2. ∗P < 0.05 (Mann–Whitney U-test) compared to si-NC group, and #P < 0.05 (Mann–Whitney U-test) compared to p-ZEB1-AS1 group.
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