AB97505

Anti-LRPPRC/GP130 antibody

Name: Anti-LRPPRC/GP130 antibody (ab97505) | Abcam
Brand: Abcam Limited
SKU: AB97505
Price: 565 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(20 Publications)

Rabbit Polyclonal LRPPRC/GP130 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 20 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LRPPRC aa 600-950.

View Alternative Names

LRP130, LRPPRC, 130 kDa leucine-rich protein, GP130, LRP 130

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-LRPPRC/GP130 antibody (AB97505)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LRPPRC/GP130 antibody (AB97505)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa using ab97505 at 1/200 dilution. Lower panel is also merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPPRC/GP130 antibody (AB97505)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPPRC/GP130 antibody (AB97505)

Paraffin embedded human colon cancer tissue stained for LRP130 using ab97505 at 1/100 at dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPPRC/GP130 antibody (AB97505)

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft using ab97505 at 1/100 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LRPPRC/GP130 antibody (AB97505)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling LRP130 protein at mitochondria using ab97505 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).
Scale bar= 10 µm.

Unknown

Western blot - Anti-LRPPRC/GP130 antibody (AB97505)

5% SDS-PAGE

All lanes:

Western blot - Anti-LRPPRC/GP130 antibody (ab97505) at 1/1000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Predicted band size: 158 kDa

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Supplier Data

Western blot - Anti-LRPPRC/GP130 antibody (AB97505)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-LRPPRC/GP130 antibody (ab97505) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysates at 50 µg

Lane 2:

Rat brain tissue lysates at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 158 kDa

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CiteAb

Western blot - Anti-LRPPRC/GP130 antibody (AB97505)

LRPPRC/GP130 western blot using anti-LRPPRC/GP130 antibody ab97505. Publication image and figure legend from Zanon, A., Rakovic, A., et al., 2013, PLoS One, PubMed 24244333.

ab97505 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab97505 please see the product overview.

Co-immunoprecipitation assays for Parkin and candidate binding proteins.Extracts from untransfected HEK293T cells were subjected to immunoprecipitation with anti-Parkin or control IgG antibodies, followed by Western Blot of input and immunoprecipitation (IP) fractions with antibodies against LRPPRC and TOMM70A. The asterisk indicates a non-specific band.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LRPPRC aa 600-950. The exact immunogen used to generate this antibody is proprietary information.

P42704

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. Positively modulates nuclear export of mRNAs containing the EIF4E sensitivity element (4ESE) by binding simultaneously to both EIF4E and the 4ESE and acting as a platform for assembly for the RNA export complex (PubMed : 19262567, PubMed : 28325843). Also binds to exportin XPO1/CRM1 to engage the nuclear pore and traffic the bound mRNAs to the cytoplasm (PubMed : 28325843). May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA (By similarity). Required for maintaining mitochondrial potential (PubMed : 23822101). Suppresses the initiation of basal levels of autophagy and mitophagy by sustaining BCL2 levels (PubMed : 23822101).

See full target information LRPPRC

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40523799

2025

Transcription arrest induces formation of RNA granules in mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Katja G Hansen,Autum Baxter-Koenigs,Caroline Am Weiss,Erik McShane,L Stirling Churchman

Translational exercise biomedicine 2:9-20 PubMed40224168

2025

Mitochondrial and cardiovascular responses to aerobic exercise training in supine and upright positions in healthy young adults: a randomized parallel arm trial.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Preobrazenski,Stuart P S Mladen,Ejaz Causer,Eveline Menezes,Hashim Islam,Patrick J Drouin,Michael E Tschakovsky,Brendon J Gurd

Journal of Cancer 16:227-240 PubMed39744573

2025

Demethylzeylasteral inhibits oxidative phosphorylation complex biogenesis by targeting LRPPRC in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Wang,Wei Zhou,Wenxi Wang,Yuxin Liang,Qiqi Xue,Zhen Zhang,Jinghe Yuan,Xiaohong Fang

Nature cell biology 25:1575-1589 PubMed37770567

2023

Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas J Kramer,Gyan Prakash,R Stefan Isaac,Karine Choquet,Iliana Soto,Boryana Petrova,Hope E Merens,Naama Kanarek,L Stirling Churchman

Developmental cell 58:2597-2613.e4 PubMed37673063

2023

Mitochondrial leak metabolism induces the Spemann-Mangold Organizer via Hif-1α in Xenopus.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra MacColl Garfinkel,Nelli Mnatsakanyan,Jeet H Patel,Andrea E Wills,Amy Shteyman,Peter J S Smith,Kambiz N Alavian,Elizabeth Ann Jonas,Mustafa K Khokha

Nature communications 14:4212 PubMed37452037

2023

The RNA-binding protein LRPPRC promotes resistance to CDK4/6 inhibition in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhou,Wenxi Wang,Yuxin Liang,Ruibin Jiang,Fensheng Qiu,Xiying Shao,Yang Liu,Le Fang,Maowei Ni,Chenhuan Yu,Yue Zhao,Weijia Huang,Jiong Li,Michael J Donovan,Lina Wang,Juan Ni,Dachi Wang,Ting Fu,Jianguo Feng,Xiaojia Wang,Weihong Tan,Xiaohong Fang

Zhongguo fei ai za zhi = Chinese journal of lung cancer 25:311-322 PubMed35599007

2022

[Comprehensive Analysis of the Relationship between m6A Methylation Patterns and Immune Microenvironment in Lung Adenocarcinoma].

Applications

Unspecified application

Species

Unspecified reactive species

Ji Ke,Jian Cui,Xingguo Yang,Xin Du,Bobo Ma,Lei Yu

Frontiers in genetics 12:817672 PubMed35237297

2022

Mitochondrial-Associated Protein LRPPRC is Related With Poor Prognosis Potentially and Exerts as an Oncogene Maintaining Mitochondrial Function in Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Jun Luo,Yuchen Li,Yanrong Lu,Yi Zhang,Bole Tian,Ziyi Zhao,Qiong-Ying Hu

Molecular and cellular biology 41:e0010321 PubMed33941617

2021

The Mammalian Ecdysoneless Protein Interacts with RNA Helicase DDX39A To Regulate Nuclear mRNA Export.

Applications

Unspecified application

Species

Unspecified reactive species

Irfana Saleem,Sameer Mirza,Aniruddha Sarkar,Mohsin Raza,Bhopal Mohapatra,Insha Mushtaq,Jun Hyun Kim,Nitish K Mishra,Mansour A Alsaleem,Emad A Rakha,Fang Qiu,Chittibabu Guda,Hamid Band,Vimla Band

Oncology reports 45: PubMed33649818

2021

LRPPRC contributes to the cisplatin resistance of lung cancer cells by regulating expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfeng Hu,Jie Cui,Lei Jin,Yani Su,Xiaozhi Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LRPPRC/GP130 antibody

Immunocytochemistry/ Immunofluorescence - Anti-LRPPRC/GP130 antibody (AB97505)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPPRC/GP130 antibody (AB97505)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPPRC/GP130 antibody (AB97505)

Immunocytochemistry/ Immunofluorescence - Anti-LRPPRC/GP130 antibody (AB97505)

Western blot - Anti-LRPPRC/GP130 antibody (AB97505)

Western blot - Anti-LRPPRC/GP130 antibody (AB97505)

Western blot - Anti-LRPPRC/GP130 antibody (AB97505)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (20)

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