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AB150376

Anti-LRRC15 antibody [EPR8188(2)]

4

(4 Reviews)

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(11 Publications)

Anti-LRRC15 antibody [EPR8188(2)] (ab150376) is a rabbit monoclonal antibody detecting LRRC15 in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat.

- Clone EPR8188(2) is the most cited clone to LRRC15
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

LIB, LRRC15, Leucine-rich repeat-containing protein 15, Leucine-rich repeat protein induced by beta-amyloid homolog, hLib

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] (AB150376)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] (AB150376)

Immunocytochemistry/ Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling LRRC15 with purified ab150376 at 1 : 100 dilution (9.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] (AB150376)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] (AB150376)

Immunofluorescent analysis of SH-SY5Y cells labeling LRRC15 with ab150376 (unpurified) at a 1/50 dilution.

Western blot - Anti-LRRC15 antibody [EPR8188(2)] (AB150376)
  • WB

Supplier Data

Western blot - Anti-LRRC15 antibody [EPR8188(2)] (AB150376)

All lanes:

Western blot - Anti-LRRC15 antibody [EPR8188(2)] (ab150376) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Human brain lysates at 20 µg

Lane 3:

Mouse brain lysates at 20 µg

Lane 4:

Rat brain lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

Western blot - Anti-LRRC15 antibody [EPR8188(2)] (AB150376)
  • WB

Unknown

Western blot - Anti-LRRC15 antibody [EPR8188(2)] (AB150376)

All lanes:

Western blot - Anti-LRRC15 antibody [EPR8188(2)] (ab150376) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

fetal brain cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit antibody at 1/2000 dilution

Predicted band size: 64 kDa

false

  • Carrier free

    Anti-LRRC15 antibody [EPR8188(2)] - BSA and Azide free

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-LRRC15 antibody [EPR8188(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-LRRC15 antibody [EPR8188(2)]

  • 660 APC

    APC Anti-LRRC15 antibody [EPR8188(2)]

  • 578 PE

    PE Anti-LRRC15 antibody [EPR8188(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-LRRC15 antibody [EPR8188(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8188(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Product Specifications

Anti-LRRC15 antibody [EPR8188(2)] (ab150376) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, WB in human, mouse, rat samples.
Anti-LRRC15 antibody [EPR8188(2)] (ab150376) specifically detects LRRC15 (UniProt ID: Q8TF66; Molecular weight: 62kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-LRRC15 antibody [EPR8188(2)] (ab150376) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.



Related Products
Conjugation-ready, carrier free format available for antibody clone EPR8188(2) - ab209427.
Antibody clone EPR8188(2) is also available pre-conjugated to a variety of labels for your convenience - PE, APC, Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555 (ab318380, ab318483, ab318586, ab318689, ab318892).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

(Microbial infection) Modulates the ability of SARS-CoV-2 to infect host cells through interaction with the spike protein (PubMed : 36228039, PubMed : 36735681, PubMed : 36757924). Does not act as a SARS-CoV-2 entry receptor but sequesters virions and antagonizes in trans SARS-CoV-2 infection of ACE2(+) cells when expressed on nearby cells (PubMed : 36228039, PubMed : 36757924).
See full target information LRRC15
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40806274

2025

Hormonal Signaling and Follicular Regulation in Normal and Miniature Pigs During Corpus Luteum Regression.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Hwan Kim

Immunity, inflammation and disease 13:e70220 PubMed40642947

2025

Leucine-Rich Repeat Containing 15 Promotes the Inflammatory Response in Rheumatoid Arthritis by Regulating NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Xin,Guangtao Xia,Xin Guan,Guangmin Xi,Min Fu

Nature communications 15:9097 PubMed39438438

2024

Single-cell sequencing reveals immune features of treatment response to neoadjuvant immunochemotherapy in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenlin Yang,He Tian,Xiaowei Chen,Bozhao Li,Guangyu Bai,Qingyuan Cai,Jiachen Xu,Wei Guo,Shuaibo Wang,Yue Peng,Qing Liang,Liyan Xue,Shugeng Gao

Nature communications 14:2353 PubMed37095087

2023

HAPLN1 potentiates peritoneal metastasis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Wiedmann,Francesca De Angelis Rigotti,Nuria Vaquero-Siguero,Elisa Donato,Elisa Espinet,Iris Moll,Elisenda Alsina-Sanchis,Hanibal Bohnenberger,Elena Fernandez-Florido,Ronja Mülfarth,Margherita Vacca,Jennifer Gerwing,Lena-Christin Conradi,Philipp Ströbel,Andreas Trumpp,Carolin Mogler,Andreas Fischer,Juan Rodriguez-Vita

PLoS biology 21:e3001959 PubMed36735681

2023

LRRC15 mediates an accessory interaction with the SARS-CoV-2 spike protein.

Applications

Unspecified application

Species

Unspecified reactive species

Jarrod Shilts,Thomas W M Crozier,Ana Teixeira-Silva,Ildar Gabaev,Pehuén Pereyra Gerber,Edward J D Greenwood,Samuel James Watson,Brian M Ortmann,Christian M Gawden-Bone,Tekle Pauzaite,Markus Hoffmann,James A Nathan,Stefan Pöhlmann,Nicholas J Matheson,Paul J Lehner,Gavin J Wright

Molecules and cells 46:231-244 PubMed36625319

2023

RUNX1 Ameliorates Rheumatoid Arthritis Progression through Epigenetic Inhibition of LRRC15.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Ding,Xiaoliang Mei,Lintao Li,Peng Fang,Ting Guo,Jianning Zhao

BioMed research international 2022:8023779 PubMed36317112

2022

Identification and Validation of Hub Genes for Predicting Treatment Targets and Immune Landscape in Rheumatoid Arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinling He,Ji Yin,Mingfang Yu,Haoyu Wang,Jiao Qiu,Aiyang Wang,Xueyi He,Xiao Wu

PLoS biology 20:e3001805 PubMed36228039

2022

LRRC15 inhibits SARS-CoV-2 cellular entry in trans.

Applications

Unspecified application

Species

Unspecified reactive species

Jaewon Song,Ryan D Chow,Mario A Peña-Hernández,Li Zhang,Skylar A Loeb,Eui-Young So,Olin D Liang,Ping Ren,Sidi Chen,Craig B Wilen,Sanghyun Lee

bioRxiv : the preprint server for biology : PubMed34845449

2021

LRRC15 is an inhibitory receptor blocking SARS-CoV-2 spike-mediated entry .

Applications

Unspecified application

Species

Unspecified reactive species

Jaewon Song,Ryan D Chow,Mario Pena-Hernandez,Li Zhang,Skylar A Loeb,Eui-Young So,Olin D Liang,Craig B Wilen,Sanghyun Lee

Cancer discovery 10:232-253 PubMed31699795

2019

Single-Cell RNA Sequencing Reveals Stromal Evolution into LRRC15 Myofibroblasts as a Determinant of Patient Response to Cancer Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia X Dominguez,Sören Müller,Shilpa Keerthivasan,Hartmut Koeppen,Jeffrey Hung,Sarah Gierke,Beatrice Breart,Oded Foreman,Travis W Bainbridge,Alessandra Castiglioni,Yasin Senbabaoglu,Zora Modrusan,Yuxin Liang,Melissa R Junttila,Christiaan Klijn,Richard Bourgon,Shannon J Turley
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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