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AB209427

Anti-LRRC15 antibody [EPR8188(2)] - BSA and Azide free

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(1 Publication)

Anti-LRRC15 antibody [EPR8188(2)] - BSA and Azide free (ab209427) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting LRRC15 in Western Blot, ICC/IF. Suitable for Human, Rat.

- Clone EPR8188(2) is the most cited clone to LRRC15
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

LIB, LRRC15, Leucine-rich repeat-containing protein 15, Leucine-rich repeat protein induced by beta-amyloid homolog, hLib

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] - BSA and Azide free (AB209427)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] - BSA and Azide free (AB209427)

Immunocytochemistry/ Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling LRRC15 with purified ab150376 at 1 : 100 dilution (9.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Unconjugated

    Anti-LRRC15 antibody [EPR8188(2)]

  • 660 APC

    APC Anti-LRRC15 antibody [EPR8188(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-LRRC15 antibody [EPR8188(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-LRRC15 antibody [EPR8188(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-LRRC15 antibody [EPR8188(2)]

  • 578 PE

    PE Anti-LRRC15 antibody [EPR8188(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8188(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-LRRC15 antibody [EPR8188(2)] - BSA and Azide free (ab209427) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Rat samples.

What is the molecular weight of LRRC15?
Anti-LRRC15 [EPR8188(2)] - BSA and Azide free (ab209427) specifically detects a band for LRRC15 (UniProt: Q8TF66) at a molecular weight of 64kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR8188(2)] also available for your convenience: ab150376, Carrier free - ab209427, PE - ab318380, APC - ab318483, Alexa Fluor® 488 - ab318586, Alexa Fluor® 647 - ab318689, Alexa Fluor® 555 - ab318892

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

(Microbial infection) Modulates the ability of SARS-CoV-2 to infect host cells through interaction with the spike protein (PubMed : 36228039, PubMed : 36735681, PubMed : 36757924). Does not act as a SARS-CoV-2 entry receptor but sequesters virions and antagonizes in trans SARS-CoV-2 infection of ACE2(+) cells when expressed on nearby cells (PubMed : 36228039, PubMed : 36757924).
See full target information LRRC15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2410229 PubMed39804980

2025

Molecular Determinants of Protein Pathogenicity at the Single-Aggregate Level.

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Urbanek,Emma F Garland,Emily E Prescott,Marianne C King,Anna Olerinyova,Hollie E Wareing,Nia Georgieva,Ellie L Bradshaw,Svetomir B Tzokov,Alexander Knight,Alexander I Tartakovskii,Tarja Malm,J Robin Highley,Suman De
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com