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AB186416

Anti-LRRC20 antibody [EP9977(2)]

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(1 Publication)

Rabbit Monoclonal LRRC20 antibody. Suitable for IP, WB and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

UNQ2429/PRO4989, LRRC20, Leucine-rich repeat-containing protein 20

3 Images
Western blot - Anti-LRRC20 antibody [EP9977(2)] (AB186416)
  • WB

Supplier Data

Western blot - Anti-LRRC20 antibody [EP9977(2)] (AB186416)

All lanes:

Western blot - Anti-LRRC20 antibody [EP9977(2)] (ab186416) at 1/2000 dilution

Lane 1:

PC3 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false

Western blot - Anti-LRRC20 antibody [EP9977(2)] (AB186416)
  • WB

Supplier Data

Western blot - Anti-LRRC20 antibody [EP9977(2)] (AB186416)

All lanes:

Western blot - Anti-LRRC20 antibody [EP9977(2)] (ab186416) at 1/2000 dilution

Lane 1:

Rat heart tissue lysate at 10 µg

Lane 2:

C6 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false

Immunoprecipitation - Anti-LRRC20 antibody [EP9977(2)] (AB186416)
  • IP

Supplier Data

Immunoprecipitation - Anti-LRRC20 antibody [EP9977(2)] (AB186416)

Western blot analysis of LRRC20 in immunoprecipitation pellets from PC3 lysate. Lane 1 : ab186416 used at 1/50 dilution, Lane 2 : PBS negative control. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG

All lanes:

Immunoprecipitation - Anti-LRRC20 antibody [EP9977(2)] (ab186416)

Predicted band size: 20 kDa

false

  • Carrier free

    Anti-LRRC20 antibody [EP9977(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP9977(2)

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8572 PubMed41022734

2025

In vivo CRISPR screening in head and neck cancer reveals Uchl5 as an immunotherapy target.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Fu,Robert Saddawi-Konefka,Jordan M Chinai,Sarah Y Kim,Ashwin V Kammula,Jonathan J Perera,Aiping Jiang,Payal Tiwari,Erin N Kistler,Shiqi Tang,Sarah M Luna,Kayla J Colvin,Juan Dubrot,Seth Anderson,Rachel A Fetterman,Cun Lan Chuong,Sarah K Lane-Reticker,Collins K Cheruiyot,Audrey J Muscato,Zahra Alipour,Douglas R Adkins,Gabriel K Griffin,Bradley E Bernstein,Ann Marie Egloff,Kathleen B Yates,Rebecca D Chernock,J Silvio Gutkind,Ravindra Uppaluri,Robert T Manguso
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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