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AB284840

Anti-LRRC20 antibody [EP9977(2)] - BSA and Azide free

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Rabbit Monoclonal LRRC20 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat samples.

View Alternative Names

UNQ2429/PRO4989, LRRC20, Leucine-rich repeat-containing protein 20

3 Images
Immunoprecipitation - Anti-LRRC20 antibody [EP9977(2)] - BSA and Azide free (AB284840)
  • IP

Supplier Data

Immunoprecipitation - Anti-LRRC20 antibody [EP9977(2)] - BSA and Azide free (AB284840)

This data was developed using ab186416, the same antibody clone in a different buffer formulation.

Western blot analysis of LRRC20 in immunoprecipitation pellets from PC3 lysate. Lane 1 : ab186416 used at 1/50 dilution, Lane 2 : PBS negative control. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG

All lanes:

Immunoprecipitation - Anti-LRRC20 antibody [EP9977(2)] - BSA and Azide free (ab284840)

Predicted band size: 20 kDa

false

Western blot - Anti-LRRC20 antibody [EP9977(2)] - BSA and Azide free (AB284840)
  • WB

Supplier Data

Western blot - Anti-LRRC20 antibody [EP9977(2)] - BSA and Azide free (AB284840)

This data was developed using ab186416, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LRRC20 antibody [EP9977(2)] (<a href='/en-us/products/primary-antibodies/lrrc20-antibody-ep99772-ab186416'>ab186416</a>) at 1/2000 dilution

Lane 1:

PC3 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false

Western blot - Anti-LRRC20 antibody [EP9977(2)] - BSA and Azide free (AB284840)
  • WB

Supplier Data

Western blot - Anti-LRRC20 antibody [EP9977(2)] - BSA and Azide free (AB284840)

This data was developed using ab186416, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LRRC20 antibody [EP9977(2)] (<a href='/en-us/products/primary-antibodies/lrrc20-antibody-ep99772-ab186416'>ab186416</a>) at 1/2000 dilution

Lane 1:

Rat heart tissue lysate at 10 µg

Lane 2:

C6 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP9977(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab284840 is the carrier-free version of ab186416

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information LRRC20
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com