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AB236395

Anti-LRRC25/MAPA antibody [OTI1D9]

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(2 Publications)

Mouse Monoclonal LRRC25/MAPA antibody. Suitable for IHC-P, WB, Flow Cyt (Intra), ICC/IF and reacts with Human, African green monkey, Recombinant full length protein samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LRRC25.

View Alternative Names

MAPA, UNQ6169/PRO20174, LRRC25, Leucine-rich repeat-containing protein 25, Monocyte and plasmacytoid-activated protein

6 Images
Flow Cytometry (Intracellular) - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)

Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)

Paraffin-embedded human lymphoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)

Paraffin-embedded human lung carcinoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for LRRC25/MAPA using ab236395 at 1/150 in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with pCMV6-ENTRY LRRC25/MAPA labeling LRRC25/MAPA using ab236395 at 1/100 dilution in ICC/IF.

Western blot - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)
  • WB

Supplier Data

Western blot - Anti-LRRC25/MAPA antibody [OTI1D9] (AB236395)

All lanes:

Western blot - Anti-LRRC25/MAPA antibody [OTI1D9] (ab236395) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY LRRC25/MAPA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Predicted band size: 33 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1D9

Isotype

IgG1

Carrier free

No

Reacts with

Human, African green monkey

Applications

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LRRC25.

Q8N386

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Recombinant full length protein": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Clone OTI1D9 (formerly 1D9).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LRRC25 also known as MAPA is a protein characterized by leucine-rich repeat motifs that facilitate protein-protein interactions. This protein often acts as a receptor within cellular processes. Its molecular mass is approximately 73 kDa. You can find LRRC25 expression in various tissues with a concentration in the immune and epithelial cells reflecting its role in the immune response.
Biological function summary

The protein carries out functions connected to the innate immune system. LRRC25 is involved in modulating cellular responses to external stimuli like pathogens. It is a part of the complex responsible for the immune response working alongside Toll-like receptors. This involvement allows LRRC25 to participate actively in recognizing and responding to microbial infections suggesting its importance for immune activation.

Pathways

LRRC25 plays a role in signaling cascades such as the NF-kB pathway. Its interaction with proteins like MyD88 illustrates its role in signaling pathways leading to inflammation and immune responses. LRRC25 acts as a link between pathogen recognition and downstream transcriptional responses ensuring the proper defense mechanism of the cells.

An altered expression or malfunction of LRRC25 has links to autoimmune diseases and chronic inflammation conditions. For example studies associate LRRC25 with rheumatoid arthritis where it interacts with inflammatory cytokines through the modulation of NF-kB activity. Also researchers observe its association with systemic lupus erythematosus where it may influence the disease's progression by affecting the immune response.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the inhibition of RLR-mediated type I interferon signaling pathway by targeting RIGI for autophagic degradation. Interacts specifically with ISG15-associated RIGI to promote interaction between RIGI and the autophagic cargo receptor p62/SQSTM1 to mediate RIGI degradation via selective autophagy (PubMed : 29288164). Also plays a role in the inhibition of NF-kappa-B signaling pathway and inflammatory response by promoting the degradation of p65/RELA.
See full target information LRRC25
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Digestive diseases and sciences 70:1395-1410 PubMed39961962

2025

LRRC25 Is a Potential Biomarker for Predicting Immunotherapy Response in Patients with Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhensong Yang,Bin Yu,Jinchen Hu,Lixin Jiang,Mi Jian

Journal of experimental & clinical cancer research : CR 41:13 PubMed34996491

2022

INPP5F translocates into cytoplasm and interacts with ASPH to promote tumor growth in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qianlei Zhou,Jianhong Lin,Yongcong Yan,Shiyu Meng,Hao Liao,Ruibin Chen,Gui He,Yue Zhu,Chuanchao He,Kai Mao,Jie Wang,Jianlong Zhang,Zhenyu Zhou,Zhiyu Xiao
View all publications
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Product promise

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