AB194813

Anti-LRRC32 antibody [GARP5]

Name: Anti-LRRC32 antibody [GARP5] (ab194813) | Abcam
Brand: Abcam Limited
SKU: AB194813
Price: 520 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Mouse Monoclonal LRRC32 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human LRRC32.

View Alternative Names

D11S833E, LRRC32, Transforming growth factor beta activator LRRC32, Garpin, Glycoprotein A repetitions predominant, Leucine-rich repeat-containing protein 32, GARP

1 Images

Lab

Western blot - Anti-LRRC32 antibody [GARP5] (AB194813)

Samples were resolved using 10% SDS-PAGE gel. Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 was used as a loading control at 0.1 µg/ml.

All lanes:

Western blot - Anti-LRRC32 antibody [GARP5] (ab194813) at 2 µg/mL

Lane 1:

Thrombocytes

Lane 2:

Leukocytes

Lane 3:

Jurkat cells

Secondary

All lanes:

IRDye 800CW Goat-anti-Mouse IgG

Observed band size: 80 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GARP5

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Native Full Length Protein corresponding to Human LRRC32.

Q14392

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab194813 was purified from cell culture supernatant by protein-A affinity chromatography. Purity is > 95% (by SDS-PAGE).

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LRRC32 also known as GARP (Glycoprotein A Repetitions Predominant) is a membrane protein with an approximate mass of 80 kDa. Scientists observe its expression mostly on regulatory T cells (Tregs) platelets and activated B cells. As an integral membrane protein LRRC32 plays an important role in cellular processes by anchoring latent transforming growth factor-beta (TGF-β) on the cell surface which is important for its regulation and activation.

Biological function summary

LRRC32 functions as part of a larger protein complex that regulates immune responses. By controlling the availability of TGF-β LRRC32 contributes directly to maintaining immune homeostasis and tolerance. Its presence on Tregs enables these cells to modulate immune responses effectively preventing excessive immune activity which could lead to autoimmune disorders. Hence LRRC32 serves as an important component in immune regulation through its interaction with and presentation of TGF-β.

Pathways

The activity of LRRC32 aligns with critical immune signaling pathways including the TGF-β signaling pathway and the FoxP3 regulatory pathway. In the TGF-β pathway it associates with other proteins like TGF-β receptor to mediate downstream signaling that influences cell growth differentiation and immune suppression. Additionally through its indirect interaction with the FoxP3 regulatory network LRRC32 aids in stabilizing Tregs and promoting their suppressive function within the immune system which involves the lineage-specific transcription factor FoxP3.

LRRC32 is relevant in conditions such as cancer and autoimmune diseases. Its association with TGF-β and FoxP3 makes it a potential target in cancer where excessive TGF-β activity might promote tumor progression and immune evasion. Conversely inadequate function of LRRC32 or TGF-β signaling could contribute to autoimmune diseases by failing to suppress harmful immune responses effectively. Its relation to GARP and TGF-β highlights its significant role in both the initiation and progression of these diseases direct implication in disease pathology and therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space (PubMed : 19651619, PubMed : 19750484, PubMed : 22278742). Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta (PubMed : 22278742). Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta (PubMed : 22278742). Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs) (PubMed : 19651619, PubMed : 19750484). Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (By similarity).

See full target information LRRC32

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 6:366 PubMed34711804

2021

Pancreatic cancer cells render tumor-associated macrophages metabolically reprogrammed by a GARP and DNA methylation-mediated mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Mengwen Zhang,Xingyi Pan,Kenji Fujiwara,Noelle Jurcak,Stephen Muth,Jiaojiao Zhou,Qian Xiao,Anqi Li,Xu Che,Zihai Li,Lei Zheng

Molecular biology of the cell 23:1129-39 PubMed22278742

2012

GARP regulates the bioavailability and activation of TGFβ.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wang,Jianghai Zhu,Xianchi Dong,Minlong Shi,Chafen Lu,Timothy A Springer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-LRRC32 antibody [GARP5]

Western blot - Anti-LRRC32 antibody [GARP5] (AB194813)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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