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AB121842

Anti-LRRC45 antibody

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(1 Publication)

Rabbit Polyclonal LRRC45 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LRRC45 aa 350-550.

View Alternative Names

Leucine-rich repeat-containing protein 45, LRRC45

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC45 antibody (AB121842)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC45 antibody (AB121842)

ab121842 at 1/450 dilution staining LRRC45 in Paraffin-embedded Human Placenta tissue by Immunohistochemistry.

Western blot - Anti-LRRC45 antibody (AB121842)
  • WB

Unknown

Western blot - Anti-LRRC45 antibody (AB121842)

All lanes:

Western blot - Anti-LRRC45 antibody (ab121842) at 1/250 dilution

Lane 1:

RT 4 lysate

Lane 2:

U251 MG lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human LRRC45 aa 350-550. The exact immunogen used to generate this antibody is proprietary information.

Q96CN5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LRRC45 also known as Leucine-Rich Repeat-Containing Protein 45 is a component of the centrosome an organelle critical for cell division. The protein has a molecular mass of about 91 kDa. It is ubiquitously expressed throughout various tissues and cells especially those with a rapid turnover. The main mechanical role of LRRC45 is organizing microtubules during the cell cycle ensuring correct chromosome segregation. Its alternate configuration sometimes noted as C10orf90 may have similar functional characteristics.
Biological function summary

LRRC45 is an important component in maintaining centrosome integrity. It functions as part of the centrosomal protein complex where it interacts closely with other centrosomal proteins to regulate their localization and stability. By stabilizing microtubules LRRC45 ensures proper anchoring of spindle fibers and helps mediate centrosome cohesion. These actions are essential for accurate mitosis and cellular proliferation.

Pathways

LRRC45 plays a role in the cell cycle and specifically in the centrosome cycle. It participates in pathways involving centrosome duplication and separation integral for proper cell division. It works closely with proteins such as Cep152 and Plk4 which initiate centrosome biogenesis. The integration of LRRC45 within these pathways highlights its importance in maintaining genomic stability across cell generations.

Defects or mutations in LRRC45 have been linked to certain forms of cancer and microcephaly. The protein's vital role in centrosome dynamics positions it as a point of interest in disorders characterized by aberrant cell division. It interacts with proteins including CEP63 that are implicated in similar pathologies suggesting a complex network where LRRC45 plays a significant role. Understanding its contributions to these disorders may provide insights into novel therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the proteinaceous fiber-like linker between two centrioles, required for centrosome cohesion.
See full target information LRRC45
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36834940

2023

Inhibition of Ribosomal RNA Processing 15 Homolog (RRP15) Suppressed Tumor Growth, Invasion and Epithelial to Mesenchymal Transition (EMT) of Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zirong Deng,Yun Xu,Yuchen Cai,Weiling Lin,Libei Zhang,Aoqing Jiang,Yuhang Zhou,Rui Zhao,Heyan Zhao,Zhaoguo Liu,Tingdong Yan
View all publications
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Product promise

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