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AB284843

Anti-LRRC49 antibody [EP13611] - BSA and Azide free

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Rabbit Monoclonal LRRC49 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

CSTPP2, LRRC49, Leucine-rich repeat-containing protein 49, Centriolar satellite-associated tubulin polyglutamylase complex regulator 2, Tubulin polyglutamylase complex subunit 4, PGs4

2 Images
Western blot - Anti-LRRC49 antibody [EP13611] - BSA and Azide free (AB284843)
  • WB

Supplier Data

Western blot - Anti-LRRC49 antibody [EP13611] - BSA and Azide free (AB284843)

This data was developed using ab189250, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LRRC49 antibody [EP13611] (<a href='/en-us/products/primary-antibodies/lrrc49-antibody-ep13611-ab189250'>ab189250</a>) at 1/5000 dilution

All lanes:

U-87 MG cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

Western blot - Anti-LRRC49 antibody [EP13611] - BSA and Azide free (AB284843)
  • WB

Supplier Data

Western blot - Anti-LRRC49 antibody [EP13611] - BSA and Azide free (AB284843)

This data was developed using ab189250, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LRRC49 antibody [EP13611] (<a href='/en-us/products/primary-antibodies/lrrc49-antibody-ep13611-ab189250'>ab189250</a>) at 1/1000 dilution

All lanes:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

Predicted band size: 79 kDa

Observed band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP13611

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab284843 is the carrier-free version of ab189250

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LRRC49 also known as Leucine Rich Repeat Containing 49 is a protein that plays a role in cellular processes through its structural features. The protein consists of leucine-rich repeats which facilitate protein-protein interactions. LRRC49 carries an approximate molecular mass of 71 kDa. Expression of LRRC49 is observed in various tissues including the brain heart and skeletal muscle. This widespread expression suggests a role in multiple physiological contexts.
Biological function summary

LRRC49 influences cellular differentiation and signaling pathways. It functions as part of larger protein complexes possibly enhancing its ability to modulate cellular functions. These interactions allow LRRC49 to regulate mechanisms important for maintaining cellular stability and adaptation potentially affecting cellular responses to environmental changes.

Pathways

LRRC49 integrates into the Wnt signaling and Notch signaling pathways both important for cell development and communication. It interacts with other proteins such as Dishevelled proteins in the Wnt pathway and possibly with Notch receptors in the Notch pathway. These interactions contribute to maintaining the balance between cell proliferation and differentiation essential for tissue and organ development.

LRRC49 may have implications in cardiac hypertrophy and certain neurological disorders. Studies suggest that abnormal expression of LRRC49 contributes to the pathophysiology of these conditions. Connections between LRRC49 and proteins like cardiomyocyte enhancer factor 2 (MEF2) in cardiac hypertrophy show potential pathways through which the protein modulates disease development. Understanding these associations aids in identifying therapeutic targets for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the tubulin polyglutamylase complex (TPGC). The complex mediates cilia and flagella polyglutamylation which is essential for their biogenesis and motility.
See full target information LRRC49
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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