AB184143

Anti-LRRC59 antibody

Name: Anti-LRRC59 antibody (ab184143) | Abcam
Brand: Abcam Limited
SKU: AB184143

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(2 Publications)

Rabbit Polyclonal LRRC59 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LRRC59 aa 50 to C-terminus.

View Alternative Names

PRO1855, LRRC59, Leucine-rich repeat-containing protein 59, Ribosome-binding protein p34, p34

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC59 antibody (AB184143)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC59 antibody (AB184143)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling LRRC59 with ab184143 at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC59 antibody (AB184143)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling LRRC59 with ab184143 at 1/500 dilution.

Supplier Data

Western blot - Anti-LRRC59 antibody (AB184143)

10% SDS-PAGE

All lanes:

Western blot - Anti-LRRC59 antibody (ab184143) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

Hela whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Predicted band size: 34 kDa

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Lab

Western blot - Anti-LRRC59 antibody (AB184143)

All lanes:

Western blot - Anti-LRRC59 antibody (ab184143) at 1/1000 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

LRRC59 Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 34 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LRRC59 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96AG4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein LRRC59 also known as Leucine-Rich Repeat-Containing Protein 59 is involved in several cellular processes. This protein has a molecular mass of approximately 40 kDa and is expressed in various tissues including the liver kidney and brain. LRRC59 contains multiple leucine-rich repeats which are structural motifs contributing to protein-protein interactions. These interactions are important for the protein's function within the cell.

Biological function summary

LRRC59 is involved in nuclear import pathways and acts as an anchor for certain proteins that need to be translocated into the nucleus. It interacts with the importin-family proteins to facilitate the transport of proteins with nuclear localization signals. LRRC59 works as a part of a larger protein complex which includes importin and nucleoporins to control transport into the nucleus. This positioning suggests that LRRC59 has an active role in regulating gene expression by controlling the nuclear entry of transcription factors and other nuclear proteins.

Pathways

LRRC59 plays a part in cellular transport and signaling pathways. It integrates into the nuclear transport pathway directly influencing gene expression by ensuring the proper nuclear import of signaling molecules. In particular it influences the well-known MAPK signaling pathway through interactions with other proteins like importin-β. These associations emphasize LRRC59’s role in maintaining cellular homeostasis and its potential impact on several signaling cascades.

LRRC59 has been linked to specific cancers and certain neurodegenerative conditions. Abnormal expression of LRRC59 has been observed in some cancer types suggesting a role in tumor progression possibly through altered nuclear transport. Additionally LRRC59 is associated with Alzheimer's disease due to its interactions with proteins like tau which are involved in the pathological aggregation seen in this disorder. These connections highlight LRRC59 as a potential target for therapeutic interventions in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for nuclear import of FGF1, but not that of FGF2. Might regulate nuclear import of exogenous FGF1 by facilitating interaction with the nuclear import machinery and by transporting cytosolic FGF1 to, and possibly through, the nuclear pores.

See full target information LRRC59

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 23:101474 PubMed35816851

2022

Identification of leucine-rich repeat-containing protein 59 (LRRC59) located in the endoplasmic reticulum as a novel prognostic factor for urothelial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Pei,Qingfeng Zhu,Xiaoping Zhuang,Honglian Ruan,Zhiguang Zhao,Haide Qin,Qiongqiong Lin

Molecular medicine reports 23: PubMed33537816

2021

Notoginsenoside R1 induces DNA damage via PHF6 protein to inhibit cervical carcinoma cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Cai,Wenquan Wu,Longhua Guo,Yongwu Xia,Xiaoxin Jiang,Limei Zhang,Feiding Peng,Pinghong Ming

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LRRC59 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC59 antibody (AB184143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRC59 antibody (AB184143)

Western blot - Anti-LRRC59 antibody (AB184143)

Western blot - Anti-LRRC59 antibody (AB184143)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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