Anti-LRRC8A antibody

Target data

Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed : 24725410, PubMed : 24790029, PubMed : 26530471, PubMed : 26824658, PubMed : 28193731, PubMed : 29769723). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed : 24725410, PubMed : 24790029, PubMed : 26530471, PubMed : 26824658, PubMed : 28193731, PubMed : 30095067). Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (PubMed : 28193731). LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed : 26530471). In complex with LRRC8C or LRRC8E, acts as a transporter of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol : mediates both import and export of 2'-3'-cGAMP, thereby promoting transfer of 2'-3'-cGAMP to bystander cells (PubMed : 33171122). In contrast, complexes containing LRRC8D inhibit transport of 2'-3'-cGAMP (PubMed : 33171122). Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed : 24790029, PubMed : 26824658, PubMed : 28193731). Can form functional channels by itself (in vitro) (PubMed : 26824658). Involved in B-cell development : required for the pro-B cell to pre-B cell transition (PubMed : 14660746). Also required for T-cell development (By similarity). Required for myoblast differentiation : VRAC activity promotes membrane hyperpolarization and regulates insulin-stimulated glucose metabolism and oxygen consumption (By similarity). Also acts as a regulator of glucose-sensing in pancreatic beta cells : VRAC currents, generated in response to hypotonicity- or glucose-induced beta cell swelling, depolarize cells, thereby causing electrical excitation, leading to increase glucose sensitivity and insulin secretion (PubMed : 29371604). Also plays a role in lysosome homeostasis by forming functional lysosomal VRAC channels in response to low cytoplasmic ionic strength condition : lysosomal VRAC channels are necessary for the formation of large lysosome-derived vacuoles, which store and then expel excess water to maintain cytosolic water homeostasis (PubMed : 31270356, PubMed : 33139539).