AB284391

Anti-LRRC8A antibody [EPR23402-29] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal LRRC8A antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Rat, Human samples.

View Alternative Names

KIAA1437, LRRC8, SWELL1, UNQ221/PRO247, LRRC8A, Volume-regulated anion channel subunit LRRC8A, Leucine-rich repeat-containing protein 8A, Swelling protein 1, HsLRRC8A

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Supplier Data

Immunoprecipitation - Anti-LRRC8A antibody [EPR23402-29] - BSA and Azide free (AB284391)

This data was developed using ab254389, the same antibody clone in a different buffer formulation.

LRRC8A was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast), whole cell lysate 10 μg with ab254389 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254389 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : C2C12 whole cell lysate 10 μg

Lane 2 : ab254389 IP in C2C12 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab254389 in C2C12 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-LRRC8A antibody [EPR23402-29] (<a href='/en-us/products/primary-antibodies/lrrc8a-antibody-epr23402-29-ab254389'>ab254389</a>)

Predicted band size: 94 kDa

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Lab

Western blot - Anti-LRRC8A antibody [EPR23402-29] - BSA and Azide free (AB284391)

This data was developed using ab254389, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lane 1-4 : 81 seconds; Lane 5-8 : 3 minutes.

All lanes:

Western blot - Anti-LRRC8A antibody [EPR23402-29] (<a href='/en-us/products/primary-antibodies/lrrc8a-antibody-epr23402-29-ab254389'>ab254389</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 3:

C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 5:

Mouse thymus tissue lysate at 20 µg

Lane 6:

Rat thymus tissue lysate at 20 µg

Lane 7:

Mouse lung tissue lysate at 20 µg

Lane 8:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

false

Unconjugated

Anti-LRRC8A antibody [EPR23402-29]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23402-29

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab284391 the carrier-free version of ab254389.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LRRC8A protein also known as SWELL1 plays a mechanical role as a component of the volume-regulated anion channel (VRAC). It has a molecular mass of approximately 104 kDa. The protein functions in transporting chloride ions across the plasma membrane helping to maintain cellular osmotic balance. LRRC8A is widely expressed in many tissues with high levels in the kidney heart and brain indicating its broad functional relevance across different organ systems.

Biological function summary

LRRC8A forms heteromeric complexes with related proteins LRRC8B LRRC8C LRRC8D and LRRC8E. These complexes constitute the VRAC channels which are important for regulating cell volume in response to osmotic changes a process called regulatory volume decrease. LRRC8A's involvement in this process is essential for cell survival under fluctuating environmental conditions as it prevents cellular swelling and lysis by allowing ions and osmolytes to exit the cell.

Pathways

Researchers have linked LRRC8A to the PI3K-AKT signaling pathway and the apoptotic pathway. In the context of the PI3K-AKT pathway LRRC8A contributes to cell survival and growth regulation interacting with proteins like AKT. Within the apoptotic pathway through VRAC activity LRRC8A plays a role in apoptosis regulation facilitating the release of apoptogenic factors that trigger programmed cell death when necessary.

Researchers have connected LRRC8A to conditions such as ischemia-reperfusion injury and cancer. In ischemia-reperfusion injury inadequate regulation by LRRC8A can lead to excessive cell swelling and damage upon reperfusion making it a potential therapeutic target. In cancer its role in cell growth pathways implicates LRRC8A in the uncontrolled proliferation seen in malignancies. Its association with VRAC partners like LRRC8D underpins its involvement in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed : 24725410, PubMed : 24790029, PubMed : 26530471, PubMed : 26824658, PubMed : 28193731, PubMed : 29769723). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed : 24725410, PubMed : 24790029, PubMed : 26530471, PubMed : 26824658, PubMed : 28193731, PubMed : 30095067). Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (PubMed : 28193731). LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed : 26530471). In complex with LRRC8C or LRRC8E, acts as a transporter of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol : mediates both import and export of 2'-3'-cGAMP, thereby promoting transfer of 2'-3'-cGAMP to bystander cells (PubMed : 33171122). In contrast, complexes containing LRRC8D inhibit transport of 2'-3'-cGAMP (PubMed : 33171122). Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed : 24790029, PubMed : 26824658, PubMed : 28193731). Can form functional channels by itself (in vitro) (PubMed : 26824658). Involved in B-cell development : required for the pro-B cell to pre-B cell transition (PubMed : 14660746). Also required for T-cell development (By similarity). Required for myoblast differentiation : VRAC activity promotes membrane hyperpolarization and regulates insulin-stimulated glucose metabolism and oxygen consumption (By similarity). Also acts as a regulator of glucose-sensing in pancreatic beta cells : VRAC currents, generated in response to hypotonicity- or glucose-induced beta cell swelling, depolarize cells, thereby causing electrical excitation, leading to increase glucose sensitivity and insulin secretion (PubMed : 29371604). Also plays a role in lysosome homeostasis by forming functional lysosomal VRAC channels in response to low cytoplasmic ionic strength condition : lysosomal VRAC channels are necessary for the formation of large lysosome-derived vacuoles, which store and then expel excess water to maintain cytosolic water homeostasis (PubMed : 31270356, PubMed : 33139539).

See full target information LRRC8A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LRRC8A antibody [EPR23402-29] - BSA and Azide free

Immunoprecipitation - Anti-LRRC8A antibody [EPR23402-29] - BSA and Azide free (AB284391)

Western blot - Anti-LRRC8A antibody [EPR23402-29] - BSA and Azide free (AB284391)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise