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AB201188

Anti-LRRC8E antibody

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(3 Publications)

Rabbit Polyclonal LRRC8E antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LRRC8E aa 250-500.

View Alternative Names

Volume-regulated anion channel subunit LRRC8E, Leucine-rich repeat-containing protein 8E, LRRC8E

1 Images
Western blot - Anti-LRRC8E antibody (AB201188)
  • WB

Supplier Data

Western blot - Anti-LRRC8E antibody (AB201188)

All lanes:

Western blot - Anti-LRRC8E antibody (ab201188) at 1/1000 dilution

All lanes:

HepG2 cell lysate

Predicted band size: 90 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human LRRC8E aa 250-500. The exact immunogen used to generate this antibody is proprietary information.

Q6NSJ5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LRRC8E also known as Leucine-rich repeat-containing protein 8E is a part of the LRRC8 family of proteins. It has an approximate mass of 95 kDa. This protein functions as a subunit of volume-regulated anion channels (VRACs) which are important for cellular volume regulation. You can frequently find LRRC8E expressed in various tissues indicating its broad functional roles. It forms heteromeric assemblies with other LRRC8 proteins particularly LRRC8A which is necessary for VRAC functionality.
Biological function summary

LRRC8E participates in the maintenance of ionic homeostasis across cell membranes. It partners with other LRRC8 subunits to regulate the transport of ions and small molecules. LRRC8E is part of the VRAC complex that responds to osmotic stress allowing cells to expel excess ions and restore normal volume. This protein's involvement in channels influencing cell survival and apoptosis demonstrates its significant role in cellular resilience mechanisms.

Pathways

Researchers have linked LRRC8E to the regulation of the cell's response to osmotic stress and apoptosis pathways. Its participation in the VRAC channel associates it with the osmosensing pathway which is essential for cell volume regulation. Furthermore LRRC8E indirectly interacts with proteins like LRRC8A a critical mediator of VRAC's osmotic function helping cells adapt to environmental changes by modulating apoptosis when needed.

Scientists connect LRRC8E to cancer and cystic fibrosis. Dysregulation of its channel function can contribute to an abnormal cellular environment influencing cancer progression. In cystic fibrosis VRAC components including LRRC8E affect ion transport processes that are already impaired due to faulty CFTR proteins. Understanding LRRC8E's role in these contexts may reveal therapeutic targets for modulating VRAC activity to improve disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed : 24790029, PubMed : 26824658, PubMed : 28193731). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed : 24790029, PubMed : 26824658). Mediates efflux of amino acids, such as aspartate, in response to osmotic stress (PubMed : 28193731). The VRAC channel also mediates transport of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol (PubMed : 33171122). Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed : 24790029, PubMed : 26824658, PubMed : 28193731). Also plays a role in lysosome homeostasis by forming functional lysosomal VRAC channels in response to low cytoplasmic ionic strength condition : lysosomal VRAC channels are necessary for the formation of large lysosome-derived vacuoles, which store and then expel excess water to maintain cytosolic water homeostasis (PubMed : 33139539).
See full target information LRRC8E
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadr9364 PubMed39671496

2024

Regulation of volume-regulated anion channels alters sensitivity to platinum chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Lily Elizabeth R Feldman,Saswat Mohapatra,Robert T Jones,Mathijs Scholtes,Charlene B Tilton,Michael V Orman,Molishree Joshi,Cailin S Deiter,Travis P Broneske,Fangyuan Qu,Corazon Gutierrez,Huihui Ye,Eric T Clambey,Sarah Parker,Tokameh Mahmoudi,Tahlita Zuiverloon,James C Costello,Dan Theodorescu

Acta pharmacologica Sinica 45:405-421 PubMed37814123

2023

Targeting PRSS23 with tipranavir induces gastric cancer stem cell apoptosis and inhibits growth of gastric cancer via the MKK3/p38 MAPK-IL24 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Xian Xiong,Yu-Ting Li,Xiang-Yu Tan,Tie Chen,Bao-Hua Liu,Li Fu

Journal of cellular biochemistry 121:2500-2509 PubMed31691355

2019

LINC00958 facilitates cervical cancer cell proliferation and metastasis by sponging miR-625-5p to upregulate LRRC8E expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lifeng Wang,Yanbo Zhong,Baohua Yang,Yunheng Zhu,Xiuxiang Zhu,Ziyin Xia,Jun Xu,Ling Xu
View all publications
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