AB176587

Anti-LRRFIP1 antibody

Name: Anti-LRRFIP1 antibody (ab176587) | Abcam
Brand: Abcam Limited
SKU: AB176587

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(1 Publication)

Rabbit Polyclonal LRRFIP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LRRFIP1 aa 750 to C-terminus.

View Alternative Names

GCF2, TRIP, LRRFIP1, Leucine-rich repeat flightless-interacting protein 1, LRR FLII-interacting protein 1, GC-binding factor 2, TAR RNA-interacting protein

2 Images

Supplier Data

Immunoprecipitation - Anti-LRRFIP1 antibody (AB176587)

Detection of LRRFIP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176587 at 6 μg/mg lysate for IP (Lane 1). For WB detection ab176587 was used at 0.4 μg/ml. Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-LRRFIP1 antibody (ab176587)

Predicted band size: 89 kDa

false

Supplier Data

Western blot - Anti-LRRFIP1 antibody (AB176587)

All lanes:

Western blot - Anti-LRRFIP1 antibody (ab176587) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 89 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human LRRFIP1 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q32MZ4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab176587 was affinity purified using an epitope specific to LRRFIP1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LRRFIP1 also known as Leucine-Rich Repeat Flightless-Interacting Protein 1 is an important protein involved in several cellular processes. It has an approximate molecular mass of 85 kDa and is expressed in various tissues including immune cells and certain tumor tissues. The protein consists of leucine-rich repeats that play a significant role in its function serving regions that form protein-protein interactions.

Biological function summary

LRRFIP1 participates in innate immune responses and acts as a transcriptional regulator. It is often found as part of a larger protein complex that influences key inflammatory pathways affecting the expression of genes linked to immune response. This protein can modulate signaling pathways influencing cell proliferation and differentiation linking it to the regulation of inflammation and immunity.

Pathways

The function of LRRFIP1 places it within the NF-kB signaling pathway and Wnt signaling pathway. In the NF-kB pathway LRRFIP1 acts by interacting with proteins that modulate gene transcription related to inflammatory responses. In the Wnt signaling pathway it associates with other proteins to regulate processes important for cell survival and development. The relationship with these pathways demonstrates LRRFIP1’s role in maintaining cellular homeostasis and orchestrating immune responses.

LRRFIP1 is connected to autoimmune diseases and certain cancers such as breast cancer. Its deregulation can lead to abnormal immune system activity or tumor progression. The protein engages with inflammatory cytokines and regulatory proteins like STAT3 which contributes to its involvement in these disorders. The study of LRRFIP1 will advance understanding in developing targeted therapies for conditions where it plays a critical role.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding.

See full target information LRRFIP1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 14:544-554 PubMed36573325

2022

Circular RNA hsa_circ_0008003 promotes the progression of non-small-cell lung cancer by sponging miR-548I and regulating KPNA4 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Wenshu Yang,Yingying Yao,Shuai Yang,Yaoqi Ke

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LRRFIP1 antibody

Immunoprecipitation - Anti-LRRFIP1 antibody (AB176587)

Western blot - Anti-LRRFIP1 antibody (AB176587)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise