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AB229466

Anti-LRRFIP1 antibody

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(2 Publications)

Rabbit Polyclonal LRRFIP1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LRRFIP1.

View Alternative Names

GCF2, TRIP, LRRFIP1, Leucine-rich repeat flightless-interacting protein 1, LRR FLII-interacting protein 1, GC-binding factor 2, TAR RNA-interacting protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-LRRFIP1 antibody (AB229466)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LRRFIP1 antibody (AB229466)

A431 (human epidermoid carcinoma cell line) cells stained for LRRFIP1 (green) using ab229466 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Nuclear counterstain : Hoechst 33342 (blue).

Western blot - Anti-LRRFIP1 antibody (AB229466)
  • WB

Supplier Data

Western blot - Anti-LRRFIP1 antibody (AB229466)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-LRRFIP1 antibody (ab229466) at 1/5000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 89 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LRRFIP1. The exact immunogen used to generate this antibody is proprietary information.

Q32MZ4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LRRFIP1 also known as Leucine-Rich Repeat Flightless-Interacting Protein 1 is an important protein involved in several cellular processes. It has an approximate molecular mass of 85 kDa and is expressed in various tissues including immune cells and certain tumor tissues. The protein consists of leucine-rich repeats that play a significant role in its function serving regions that form protein-protein interactions.
Biological function summary

LRRFIP1 participates in innate immune responses and acts as a transcriptional regulator. It is often found as part of a larger protein complex that influences key inflammatory pathways affecting the expression of genes linked to immune response. This protein can modulate signaling pathways influencing cell proliferation and differentiation linking it to the regulation of inflammation and immunity.

Pathways

The function of LRRFIP1 places it within the NF-kB signaling pathway and Wnt signaling pathway. In the NF-kB pathway LRRFIP1 acts by interacting with proteins that modulate gene transcription related to inflammatory responses. In the Wnt signaling pathway it associates with other proteins to regulate processes important for cell survival and development. The relationship with these pathways demonstrates LRRFIP1’s role in maintaining cellular homeostasis and orchestrating immune responses.

LRRFIP1 is connected to autoimmune diseases and certain cancers such as breast cancer. Its deregulation can lead to abnormal immune system activity or tumor progression. The protein engages with inflammatory cytokines and regulatory proteins like STAT3 which contributes to its involvement in these disorders. The study of LRRFIP1 will advance understanding in developing targeted therapies for conditions where it plays a critical role.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding.
See full target information LRRFIP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 95:e0122721 PubMed34468169

2021

Nectin-1 Is an Entry Mediator for Varicella-Zoster Virus Infection of Human Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Labchan Rajbhandari,Priya Shukla,Balaji Jagdish,Abby Mandalla,Qingxue Li,Mir A Ali,Hojae Lee,Gabsang Lee,Tomohiko Sadaoka,Jeffrey I Cohen,Arun Venkatesan

Journal of vascular research 58:311-320 PubMed33882503

2021

Nuclear Factor Kappa-B/Homeobox A9-Mediated Modulation of Leucine-Rich Repeat Flightless-Interacting Protein 1 Is Involved in Advanced Glycation End Product-Induced Endothelial Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Zhu,Songjie Hu,Yunjun He,Chenyang Qiu,Tianchi Chen,Yangyan He,Ziheng Wu,Lu Tian,Tao Shang,Yilang Xiang,Hongkun Zhang,Donglin Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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