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AB228666

Anti-LRRK1 antibody

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(4 Publications)

Rabbit Polyclonal LRRK1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LRRK1.

View Alternative Names

KIAA1790, LRRK1, Leucine-rich repeat serine/threonine-protein kinase 1

1 Images
Western blot - Anti-LRRK1 antibody (AB228666)
  • WB

Supplier Data

Western blot - Anti-LRRK1 antibody (AB228666)

5% SDS-PAGE

All lanes:

Western blot - Anti-LRRK1 antibody (ab228666) at 1/1000 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 225 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human LRRK1. The exact immunogen used to generate this antibody is proprietary information.

Q38SD2

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Proteins & Peptides

AB164085

Recombinant Human LRRK1 protein

SDS-PAGE - Recombinant Human LRRK1 protein (AB164085)

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Cell Lines & Lysates

AB264726

Human LRRK1 knockout HeLa cell line

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Primary Antibodies

AB230260

Anti-RAB8A (phospho T72) antibody [MJF-R20]

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Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] (AB230260)

  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LRRK1 also known as leucine-rich repeat kinase 1 is an important protein with a molecular mass of approximately 273 kDa. This protein belongs to the family of serine/threonine kinases and functions mechanically by phosphorylating other proteins a process critical for regulating various cellular processes. It is highly expressed in bone tissues with lower levels found in the liver and brain. LRRK1 also contains a GTPase domain which contributes to its kinase activity and is sometimes studied alongside its close relative LRRK2 due to structural similarities.
Biological function summary

LRRK1 plays a significant role in bone homeostasis and remodeling contributing to skeletal development and maintenance. LRRK1 does not form part of a larger complex but influences pathways involved in cytoskeletal dynamics and cellular motility. This protein is essential for osteoclast function where its kinase activity aids in the breakdown of bone matrix. Understanding its role in bone turnover helps researchers investigate potential therapeutic targets for bone-related conditions.

Pathways

LRRK1 is involved in signaling pathways related to bone resorption and remodeling. It participates in the RANK/RANKL/OPG pathway essential for osteoclast differentiation and activation. Additionally it interacts with proteins such as c-Src and Cbl which are critical in bone matrix dissolution and cellular adhesion processes. These interactions highlight LRRK1’s pivotal role in maintaining skeletal integrity through regulated bone degradation.

Research indicates that LRRK1 can impact conditions such as osteoporosis and Paget's disease of bone. Osteoporosis involves decreased bone mass and increased fracture risk which relates to dysregulated osteoclast activity in which LRRK1 may play a part. In Paget's disease abnormal bone remodeling processes occur possibly involving dysfunctions in LRRK1 signaling. The interaction of LRRK1 with LRRK2 is also relevant in this context as both enzymes share mechanistic features that might influence bone-related pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231). Phosphorylates RAB7A; this activity is dependent on protein kinase C (PKC) activation (PubMed : 36040231, PubMed : 37558661, PubMed : 37857821). Plays a role in the negative regulation of bone mass, acting through the maturation of osteoclasts (By similarity).
See full target information LRRK1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadt2050 PubMed40465731

2025

Type II kinase inhibitors that target Parkinson's disease-associated LRRK2.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolai D Raig,Katherine J Surridge,Marta Sanz-Murillo,Verena Dederer,Andreas Krämer,Martin P Schwalm,Nicholas M Lattal,Lewis Elson,Deep Chatterjee,Sebastian Mathea,Thomas Hanke,Andres E Leschziner,Samara L Reck-Peterson,Stefan Knapp

Nature communications 14:4797 PubMed37558661

2023

Structure and regulation of full-length human leucine-rich repeat kinase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Riley D Metcalfe,Juliana A Martinez Fiesco,Luis Bonet-Ponce,Jillian H Kluss,Mark R Cookson,Ping Zhang

The Journal of clinical investigation 131: PubMed34623320

2021

T cell protein tyrosine phosphatase protects intestinal barrier function by restricting epithelial tight junction remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Ronald R Marchelletta,Moorthy Krishnan,Marianne R Spalinger,Taylaur W Placone,Rocio Alvarez,Anica Sayoc-Becerra,Vinicius Canale,Ali Shawki,Young Su Park,Lucas Hp Bernts,Stephen Myers,Michel L Tremblay,Kim E Barrett,Evan Krystofiak,Bechara Kachar,Dermot Pb McGovern,Christopher R Weber,Elaine M Hanson,Lars Eckmann,Declan F McCole

Bioscience reports 40: PubMed32242897

2020

LINC00511 exacerbated T-cell acute lymphoblastic leukemia via miR-195-5p/LRRK1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shengli Li,Wenwen Guo,Huayun Geng,Chao Wang,Shuige Yang,Xinxin Xu
View all publications
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Product promise

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