Rabbit Polyclonal LRRK1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LRRK1.
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Publications
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- Nature communications 14:47972023Structure and regulation of full-length human leucine-rich repeat kinase 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRiley D Metcalfe,Juliana A Martinez Fiesco,Luis Bonet-Ponce,Jillian H Kluss,Mark R Cookson,Ping ZhangPubMed 37558661
- Bioscience reports 40:2020LINC00511 exacerbated T-cell acute lymphoblastic leukemia via miR-195-5p/LRRK1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengli Li,Wenwen Guo,Huayun Geng,Chao Wang,Shuige Yang,Xinxin XuPubMed 32242897
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LRRK1. The exact immunogen used to generate this antibody is proprietary information. Database link Q38SD2
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231). Phosphorylates RAB7A; this activity is dependent on protein kinase C (PKC) activation (PubMed:36040231, PubMed:37558661, PubMed:37857821). Plays a role in the negative regulation of bone mass, acting through the maturation of osteoclasts (By similarity).
Alternative names
KIAA1790, LRRK1, Leucine-rich repeat serine/threonine-protein kinase 1
Recommended products
Rabbit Polyclonal LRRK1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LRRK1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LRRK1. The exact immunogen used to generate this antibody is proprietary information. Database link Q38SD2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LRRK1 also known as leucine-rich repeat kinase 1 is an important protein with a molecular mass of approximately 273 kDa. This protein belongs to the family of serine/threonine kinases and functions mechanically by phosphorylating other proteins a process critical for regulating various cellular processes. It is highly expressed in bone tissues with lower levels found in the liver and brain. LRRK1 also contains a GTPase domain which contributes to its kinase activity and is sometimes studied alongside its close relative LRRK2 due to structural similarities.
- Biological function summary
LRRK1 plays a significant role in bone homeostasis and remodeling contributing to skeletal development and maintenance. LRRK1 does not form part of a larger complex but influences pathways involved in cytoskeletal dynamics and cellular motility. This protein is essential for osteoclast function where its kinase activity aids in the breakdown of bone matrix. Understanding its role in bone turnover helps researchers investigate potential therapeutic targets for bone-related conditions.
- Pathways
LRRK1 is involved in signaling pathways related to bone resorption and remodeling. It participates in the RANK/RANKL/OPG pathway essential for osteoclast differentiation and activation. Additionally it interacts with proteins such as c-Src and Cbl which are critical in bone matrix dissolution and cellular adhesion processes. These interactions highlight LRRK1’s pivotal role in maintaining skeletal integrity through regulated bone degradation.
- Associated diseases and disorders
Research indicates that LRRK1 can impact conditions such as osteoporosis and Paget's disease of bone. Osteoporosis involves decreased bone mass and increased fracture risk which relates to dysregulated osteoclast activity in which LRRK1 may play a part. In Paget's disease abnormal bone remodeling processes occur possibly involving dysfunctions in LRRK1 signaling. The interaction of LRRK1 with LRRK2 is also relevant in this context as both enzymes share mechanistic features that might influence bone-related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-LRRK1 antibody (ab228666)
5% SDS-PAGE
All lanes: Western blot - Anti-LRRK1 antibody (ab228666) at 1/1000 dilution
All lanes: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 225 kDa
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