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Rabbit Recombinant Monoclonal LRRK2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 4 publications.

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Images

Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (AB183216), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (AB183216), expandable thumbnail
  • Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (AB183216), expandable thumbnail
  • Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (AB183216), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Please check the parent abID, Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475, for a recommended dilution.

Species
Mouse
Dilution info
-
Notes

Please check the parent abID, Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475, for a recommended dilution.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

The protein expressed by the gene LRRK2 is a serine/threonine-protein kinase that phosphorylates a wide range of proteins involved in neuronal plasticity, autophagy, and vesicle trafficking. It serves as a key regulator of RAB GTPases by affecting the GTP/GDP exchange and interaction partners of RABs through phosphorylation, targeting proteins like RAB3A, RAB3B, and others. LRRK2 manages the GDP/GTP exchange for RAB8A by phosphorylating 'Thr-72', inhibiting RAB8A's interaction with GDI1/GDI2, and influencing primary ciliogenesis to enhance SHH signaling in the brain. It works with RAB29 in retromer-dependent recycling of proteins between lysosomes and the Golgi. The protein is involved in shaping neuronal morphology in the CNS, synaptic vesicle trafficking, and recruiting SEC16A to assist in ER to Golgi transport. It enhances autophagy via the CaMKK/AMPK pathway and relates to nicotinic acid adenine dinucleotide phosphate receptors and lysosomal activity. LRRK2 phosphorylates PRDX3 and APP, influencing neuron apoptosis. Independently, it inhibits MAPT degradation, fostering MAPT oligomerization. Additionally, LRRK2 possesses GTPase activity that regulates its kinase activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

PARK8, LRRK2, Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin

Recommended products

Rabbit Recombinant Monoclonal LRRK2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
MJFF3 (c69-6)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab183216 is the carrier-free version of Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475.

Well-characterized antibodies to efficiently detect and purify LRRK2 protein are a critical need in the Parkinson's Disease (PD) research community. To help accelerate LRRK2 research, The Michael J. Fox Foundation (MJFF), working with Epitomics, Inc., has generated unique and high quality LRRK2 rabbit monoclonal antibodies to be widely available for PD research community.

LRRK2 (Leucine-rich repeat kinase 2, dardarin) is a protein kinase belonging to the ROCO family, which is defined by the presence of a ROC (Ras/GTPase of complex proteins) domain and COR (C-terminal of Roc) region. LRRK2 exhibits kinase activity whereby it can undergo autophosphorylation and can phosphorylate generic substrates. In addition, the GTPase domain of LRRK2 can mediate GDP (guanosine-5′-diphosphate)/GTP (guanosine-5′-triphosphate) binding as well as GTP hydrolysis.

LRRK2 is mutated in a significant number of Parkinson's disease (PD) patients. Mutations in this gene account for 4% of PD, and are observed in 1% of sporadic PD patients. Clinical symptoms of patients carrying PD-associated mutations of LRRK2 are indistinguishable from typical sporadic PD. The spectra of neuropathological features of PARK8 (type 8), the type corresponding to LRRK2, is broad and appears to encompass those associated with other familial PD cases such as PARK1 (alpha-synuclein) and PARK2 (Parkin). Patients with this gene mutation have typical relatively late onset Parkinsonism with features comparable with idiopathic PD; symptoms include asymmetric rest tremor, bradykinesia, rigidity, and a good response to 3,4-dihyroxy-l-phenylalanine (l-DOPA). The pathology of cases with LRRK2 mutations is pleomorphic.

For more characterization data and protocols using this LRRK2 Antibody, please refer to Davies, et al. 2013. Biochemical J 453(1):101-113 [PMID: 23560750]

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein LRRK2 also known as leucine-rich repeat kinase 2 or dardarin is an enzyme with a molecular weight of approximately 286 kDa. It functions as a kinase meaning it adds phosphate groups to other proteins which affects their activity. LRRK2 is expressed in various tissues but it is highly abundant in the brain especially in regions such as the striatum and cortex. It has a significant role in cellular signaling processes due to its phosphorylation activity.

Biological function summary

LRRK2 interacts with cellular mechanisms by regulating cytoskeletal dynamics autophagy and vesicle trafficking. It is a part of a larger complex that includes other proteins involved in these processes. The kinase activity of LRRK2 plays an essential part in maintaining neuronal health and function. It influences the process of autophagy which is a way cells clean themselves by removing damaged components and recycling them.

Pathways

The action of LRRK2 is central to the mitogen-activated protein kinase (MAPK) and the mammalian target of rapamycin (mTOR) pathways. In these pathways LRRK2 interacts with other proteins such as mTOR and RPS6KB1. It modulates cellular processes like growth proliferation and response to stressors. Its kinase activity affects the phosphorylation state of targets within the pathways hence influencing biological outcomes like survival and apoptosis.

Associated diseases and disorders

LRRK2 mutations have a significant connection to Parkinson's disease and Crohn's disease. In Parkinson's disease mutated LRRK2 leads to abnormal protein aggregation linking to proteins such as alpha-synuclein. For Crohn's disease LRRK2 influences the immune response and intestinal inflammation. These connections highlight LRRK2's role in the pathogenesis and contribute to understanding these complex disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (ab183216), expandable thumbnail

    Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (ab183216)

    This WB data was generated using the same anti-LRRK2 antibody clone, MJFF3 (c69-6), in a different buffer formulation (cat# Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475).

    Lane 1: A549 whole cell lysate (20 μg)
    Lane 2: MEF whole cell lysate (20 μg)
    Lane 3: A549 (KO) whole cell lysate (20 μg)
    Lane 4: MEF (KO) whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475 observed at 286 kDa. Red - loading control, ab18058, observed at 130 kDa.

    Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475 was shown to specifically react with Wild type A549 and MEF cells whilst no band was observed when knockout samples were used. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and knockout samples were subjected to SDS-PAGE. Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] (Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475)

    Predicted band size: 286 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (ab183216), expandable thumbnail
    This image is courtesy of the Douglas Walker Lab

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (ab183216)

    This IHC data was generated using the same anti-LRRK2 antibody clone, MJFF3 (c69-6), in a different buffer formulation (cat# Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475).

    Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475, at 1/200 dilution, staining LRRK2 in paraffin embedded Human hippocampus tissue using immunohistochemical analysis.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (ab183216), expandable thumbnail

    Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (ab183216)

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (ab183216)

    All lanes: NIH/3T3 (mouse embryo) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)

    Predicted band size: 286 kDa

    Exposure time: 10s

  • Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (ab183216), expandable thumbnail

    Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] - BSA and Azide free (ab183216)

    This data was developed using Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475, the same antibody clone in a different buffer formulation.

    Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475 was shown to react with LRRK2 in wild-type A549 cells in Western blot with loss of signal observed in a LRRK2 knockout cell line. Wild-type A549 and LRRK2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-LRRK2 antibody [MJFF3 (c69-6)] (Anti-LRRK2 antibody [MJFF3 (c69-6)] ab133475) at 1/1000 dilution

    Lane 1: Wild-type A549 lysate at 25 µg

    Lane 2: LRRK2 knock-out A549 lysate at 25 µg

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Product protocols

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