Rabbit Recombinant Monoclonal LRRK2 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.
Images
![Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (AB170993), expandable thumbnail](https://content.abcam.com/products/images/lrrk2-antibody-udd3-3012-bsa-and-azide-free-ab170993--western-blot-img104378.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (AB170993)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (AB170993), expandable thumbnail](https://content.abcam.com/products/images/lrrk2-antibody-udd3-3012-bsa-and-azide-free-ab170993--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img69208.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (AB170993)")
![Immunocytochemistry/ Immunofluorescence - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (AB170993), expandable thumbnail](https://content.abcam.com/products/images/lrrk2-antibody-udd3-3012-bsa-and-azide-free-ab170993--immunocytochemistry-immunofluorescence-img14272.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (AB170993)")
![Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (AB170993), expandable thumbnail](https://content.abcam.com/products/images/lrrk2-antibody-udd3-3012-bsa-and-azide-free-ab170993--western-blot-img438530.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (AB170993)")
Publications
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- Cell cycle (Georgetown, Tex.) 18:2727-27412019Silencing of long noncoding RNA SBF2-AS1 inhibits proliferation, migration and invasion and contributes to apoptosis in osteosarcoma cells by upregulating microRNA-30a to suppress FOXA1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiang-Hua Dai,Wen-Zhou Huang,Chen Li,Jun Deng,Si-Jian Lin,Jun LuoPubMed 31432728
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
The protein expressed by the gene LRRK2 is a serine/threonine-protein kinase that phosphorylates a wide range of proteins involved in neuronal plasticity, autophagy, and vesicle trafficking. It serves as a key regulator of RAB GTPases by affecting the GTP/GDP exchange and interaction partners of RABs through phosphorylation, targeting proteins like RAB3A, RAB3B, and others. LRRK2 manages the GDP/GTP exchange for RAB8A by phosphorylating 'Thr-72', inhibiting RAB8A's interaction with GDI1/GDI2, and influencing primary ciliogenesis to enhance SHH signaling in the brain. It works with RAB29 in retromer-dependent recycling of proteins between lysosomes and the Golgi. The protein is involved in shaping neuronal morphology in the CNS, synaptic vesicle trafficking, and recruiting SEC16A to assist in ER to Golgi transport. It enhances autophagy via the CaMKK/AMPK pathway and relates to nicotinic acid adenine dinucleotide phosphate receptors and lysosomal activity. LRRK2 phosphorylates PRDX3 and APP, influencing neuron apoptosis. Independently, it inhibits MAPT degradation, fostering MAPT oligomerization. Additionally, LRRK2 possesses GTPase activity that regulates its kinase activity. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
PARK8, LRRK2, Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin
Recommended products
Rabbit Recombinant Monoclonal LRRK2 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- UDD3 30(12)
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody does not give a positive signal in Human fetal brain. Please contact our Scientific Support team if you have any questions.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab170993 is the carrier-free version of Anti-LRRK2 antibody [UDD3 30(12)] ab133518.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein LRRK2 also known as leucine-rich repeat kinase 2 or dardarin is an enzyme with a molecular weight of approximately 286 kDa. It functions as a kinase meaning it adds phosphate groups to other proteins which affects their activity. LRRK2 is expressed in various tissues but it is highly abundant in the brain especially in regions such as the striatum and cortex. It has a significant role in cellular signaling processes due to its phosphorylation activity.
- Biological function summary
LRRK2 interacts with cellular mechanisms by regulating cytoskeletal dynamics autophagy and vesicle trafficking. It is a part of a larger complex that includes other proteins involved in these processes. The kinase activity of LRRK2 plays an essential part in maintaining neuronal health and function. It influences the process of autophagy which is a way cells clean themselves by removing damaged components and recycling them.
- Pathways
The action of LRRK2 is central to the mitogen-activated protein kinase (MAPK) and the mammalian target of rapamycin (mTOR) pathways. In these pathways LRRK2 interacts with other proteins such as mTOR and RPS6KB1. It modulates cellular processes like growth proliferation and response to stressors. Its kinase activity affects the phosphorylation state of targets within the pathways hence influencing biological outcomes like survival and apoptosis.
- Associated diseases and disorders
LRRK2 mutations have a significant connection to Parkinson's disease and Crohn's disease. In Parkinson's disease mutated LRRK2 leads to abnormal protein aggregation linking to proteins such as alpha-synuclein. For Crohn's disease LRRK2 influences the immune response and intestinal inflammation. These connections highlight LRRK2's role in the pathogenesis and contribute to understanding these complex disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993), expandable thumbnail](https://content.abcam.com/products/images/lrrk2-antibody-udd3-3012-bsa-and-azide-free-ab170993--western-blot-img104378.jpg "Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)")
Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)
This WB data was generated using the same anti-LRRK2 antibody clone, UDD3 30(12), in a different buffer formulation (cat# Anti-LRRK2 antibody [UDD3 30(12)] ab133518).
Lane 1: Wild-type A549 cell lysate (20 μg)
Lane 2: LRRK2 knockout A549 cell lysate (20 μg)
Lane 3: Wild-type MEF cell lysate (20 μg)
Lane 4: LRRK2 knockout MEF cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-LRRK2 antibody [UDD3 30(12)] ab133518 observed at 238 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 124 kDa.
Anti-LRRK2 antibody [UDD3 30(12)] ab133518 was shown to specifically react with wild type A549 and MEF cell lines. No band was observed when knock out samples were used. Wild-type and LRRK2 knockout samples were subjected to SDS-PAGE. Anti-LRRK2 antibody [UDD3 30(12)] ab133518 and Anti-Vinculin antibody [VIN-54] ab130007 (loading control to Vinculin) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Wild-type and LRRK2 knockout MEF and A549 cells were provide as generous a gift from Professor Dario Alessi, MRC Protein Phosphorylation and Ubiquitination Unit (University of Dundee).
All lanes: Western blot - Anti-LRRK2 antibody [UDD3 30(12)] (Anti-LRRK2 antibody [UDD3 30(12)] ab133518)
Predicted band size: 286 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993), expandable thumbnail](https://content.abcam.com/products/images/lrrk2-antibody-udd3-3012-bsa-and-azide-free-ab170993--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img69208.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)
This IHC data was generated using the same anti-LRRK2 antibody clone, UDD3 30(12), in a different buffer formulation (cat# Anti-LRRK2 antibody [UDD3 30(12)] ab133518).
Immunohistochemical staining of paraffin-embedded human kidney with purified Anti-LRRK2 antibody [UDD3 30(12)] ab133518 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993), expandable thumbnail](https://content.abcam.com/products/images/lrrk2-antibody-udd3-3012-bsa-and-azide-free-ab170993--immunocytochemistry-immunofluorescence-img14272.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)")
Immunocytochemistry/ Immunofluorescence - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)
Immunofluorescent staining of SH-SY5Y cells fixed and permeablized with 4% PFA and 0.1% Triton X 100 using purified Anti-LRRK2 antibody [UDD3 30(12)] ab133518 at a dilution of 1/200. An Alexa Fluor® 488 goat anti-rabbit was used as the secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, 1/400) and the sample was stained with DAPI. The negative control is shown in bottom right hand panel - for the negative control, purified Anti-LRRK2 antibody [UDD3 30(12)] ab133518 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LRRK2 antibody [UDD3 30(12)] ab133518).
![Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993), expandable thumbnail](https://content.abcam.com/products/images/lrrk2-antibody-udd3-3012-bsa-and-azide-free-ab170993--western-blot-img438530.jpg "Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)")
Western blot - Anti-LRRK2 antibody [UDD3 30(12)] - BSA and Azide free (ab170993)
This data was developed using Anti-LRRK2 antibody [UDD3 30(12)] ab133518, the same antibody clone in a different buffer formulation.
Anti-LRRK2 antibody [UDD3 30(12)] ab133518 was shown to react with LRRK2 in wild-type A549 cells in Western blot with loss of signal observed in a LRRK2 knockout cell line. Wild-type A549 and LRRK2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-LRRK2 antibody [UDD3 30(12)] ab133518 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.All lanes: Western blot - Anti-LRRK2 antibody [UDD3 30(12)] (Anti-LRRK2 antibody [UDD3 30(12)] ab133518) at 1/500 dilution
Lane 1: Wild-type A549 lysate at 25 µg
Lane 2: LRRK2 knock-out A549 lysate at 25 µg
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