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Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) is a rabbit monoclonal antibody that is used to detect LRRK2 in Western Blot. Suitable for Human samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 40 publications

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Images

Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (AB203181), expandable thumbnail
  • Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (AB203181), expandable thumbnail
  • Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (AB203181), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

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Target data

Function

The protein expressed by the gene LRRK2 is a serine/threonine-protein kinase that phosphorylates a wide range of proteins involved in neuronal plasticity, autophagy, and vesicle trafficking. It serves as a key regulator of RAB GTPases by affecting the GTP/GDP exchange and interaction partners of RABs through phosphorylation, targeting proteins like RAB3A, RAB3B, and others. LRRK2 manages the GDP/GTP exchange for RAB8A by phosphorylating 'Thr-72', inhibiting RAB8A's interaction with GDI1/GDI2, and influencing primary ciliogenesis to enhance SHH signaling in the brain. It works with RAB29 in retromer-dependent recycling of proteins between lysosomes and the Golgi. The protein is involved in shaping neuronal morphology in the CNS, synaptic vesicle trafficking, and recruiting SEC16A to assist in ER to Golgi transport. It enhances autophagy via the CaMKK/AMPK pathway and relates to nicotinic acid adenine dinucleotide phosphate receptors and lysosomal activity. LRRK2 phosphorylates PRDX3 and APP, influencing neuron apoptosis. Independently, it inhibits MAPT degradation, fostering MAPT oligomerization. Additionally, LRRK2 possesses GTPase activity that regulates its kinase activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

PARK8, LRRK2, Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin

Recommended products

Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) is a rabbit monoclonal antibody that is used to detect LRRK2 in Western Blot. Suitable for Human samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 40 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
MJFR-19-7-8
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of LRRK2?


Anti-LRRK2 (phospho S1292) [MJFR-19-7-8] (ab203181) specifically detects a band for LRRK2 (UniProt: Q5S007) at a molecular weight of 286kDa.

Trusted by the scientific community


Anti-LRRK2 (phospho S1292) [MJFR-19-7-8] (ab203181) was first used in a scientific publication in 2015 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [MJFR-19-7-8] also available for your convenience:
ab203181, Carrier free - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - Low endotoxin, Azide free ab206035, Carrier free - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - BSA and Azide free ab256581



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein LRRK2 also known as leucine-rich repeat kinase 2 or dardarin is an enzyme with a molecular weight of approximately 286 kDa. It functions as a kinase meaning it adds phosphate groups to other proteins which affects their activity. LRRK2 is expressed in various tissues but it is highly abundant in the brain especially in regions such as the striatum and cortex. It has a significant role in cellular signaling processes due to its phosphorylation activity.

Biological function summary

LRRK2 interacts with cellular mechanisms by regulating cytoskeletal dynamics autophagy and vesicle trafficking. It is a part of a larger complex that includes other proteins involved in these processes. The kinase activity of LRRK2 plays an essential part in maintaining neuronal health and function. It influences the process of autophagy which is a way cells clean themselves by removing damaged components and recycling them.

Pathways

The action of LRRK2 is central to the mitogen-activated protein kinase (MAPK) and the mammalian target of rapamycin (mTOR) pathways. In these pathways LRRK2 interacts with other proteins such as mTOR and RPS6KB1. It modulates cellular processes like growth proliferation and response to stressors. Its kinase activity affects the phosphorylation state of targets within the pathways hence influencing biological outcomes like survival and apoptosis.

Associated diseases and disorders

LRRK2 mutations have a significant connection to Parkinson's disease and Crohn's disease. In Parkinson's disease mutated LRRK2 leads to abnormal protein aggregation linking to proteins such as alpha-synuclein. For Crohn's disease LRRK2 influences the immune response and intestinal inflammation. These connections highlight LRRK2's role in the pathogenesis and contribute to understanding these complex disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181), expandable thumbnail
    Kluss et al NPJ Parkinsons Dis. 2018; 4: 13. Published online 2018 Apr 19. doi: 10.1038/s41531-018-0049-1

    Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)

    Characterization of pS1292 LRRK2 antibody

    Increasing amounts of HEK-293FT lysates transiently expressing WT LRRK2 (A) or S1292A LRRK2 (B) were analyzed by western blot and probed with the commercially available anti-pS1292 LRRK2 antibody (ab203181). The antibody showed minimal non-specific bands and linear detection in the range tested (b).

    From Figure1a of Kluss et al.

    Kluss et al NPJ Parkinsons Dis. 2018; 4: 13. Published online 2018 Apr 19. doi: 10.1038/s41531-018-0049-1

    Reproduced under Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/.

    All lanes: Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)

    Predicted band size: 286 kDa

  • Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181), expandable thumbnail

    Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)

    Blocking/Dilution buffer: 5% BSA/TBST.

    The image is provided by Dr. Jeremy Nicols, Parkinson's Institute, Sunnyvale, CA, USA.

    G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 4).

    Observed band size: 286 + 27 (GFP) = 313 kDa.

    All lanes: Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/mL

    Lane 1: GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose at 10 µg

    Lane 2: GFP-LRRK2 mutant D2017A transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose at 10 µg

    Lane 3: GFP-LRRK2 mutant S1292A/G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose at 10 µg

    Lane 4: GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose at 10 µg

    Secondary

    All lanes: Goat anti-rabbit (IRDye 800) at 1/10000 dilution

    Predicted band size: 286 kDa

    Observed band size: 313 kDa

  • Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181), expandable thumbnail

    Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The image is provided by Dr. Paul Davies, University of Dundee, Dundee Scotland, UK.

    G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 3).

    All lanes: Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (ab203181) at 1 µg/mL

    Lane 1: LRRK2 mutant G2019S/S1292A transfected HEK293 lysate at 10 µg

    Lane 2: LRRK2 mutant G2019S transfected HEK293 inhibitor treated lysate at 10 µg

    Lane 3: LRRK2 mutant G2019S transfected HEK293 lysate at 10 µg

    Lane 4: LRRK2 mutant S1292A transfected HEK293 lysate at 10 µg

    Lane 5: LRRK2 wt transfected HEK293 inhibitor treated lysate at 10 µg

    Lane 6: LRRK2 wt transfected HEK293 lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2500 dilution

    Predicted band size: 286 kDa

    Observed band size: 286 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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