AB256581

Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal LRRK2 phospho S1292 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

PARK8, LRRK2, Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin

2 Images

Supplier Data

Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - BSA and Azide free (AB256581)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203181).

Blocking/Dilution buffer : 5% NFDM/TBST.

The image is provided by Dr. Paul Davies, University of Dundee, Dundee Scotland, UK.

G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 3).

All lanes:

Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (<a href='/en-us/products/primary-antibodies/lrrk2-phospho-s1292-antibody-mjfr-19-7-8-ab203181'>ab203181</a>) at 1 µg/mL

Lane 1:

LRRK2 mutant G2019S/S1292A transfected HEK293 lysate at 10 µg

Lane 2:

LRRK2 mutant G2019S transfected HEK293 inhibitor treated lysate at 10 µg

Lane 3:

LRRK2 mutant G2019S transfected HEK293 lysate at 10 µg

Lane 4:

LRRK2 mutant S1292A transfected HEK293 lysate at 10 µg

Lane 5:

LRRK2 wt transfected HEK293 inhibitor treated lysate at 10 µg

Lane 6:

LRRK2 wt transfected HEK293 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2500 dilution

Predicted band size: 286 kDa

Observed band size: 286 kDa

false

Supplier Data

Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - BSA and Azide free (AB256581)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203181).

Blocking/Dilution buffer : 5% BSA/TBST.

The image is provided by Dr. Jeremy Nicols, Parkinson's Institute, Sunnyvale, CA, USA.

G2019S mutation results in an increased LRRK2 auto-phosphorylation including S1292 (lane 4).

Observed band size : 286 + 27 (GFP) = 313 kDa.

All lanes:

Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] (<a href='/en-us/products/primary-antibodies/lrrk2-phospho-s1292-antibody-mjfr-19-7-8-ab203181'>ab203181</a>) at 1 µg/mL

Lane 1:

GFP-LRRK2 wt transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose at 10 µg

Lane 2:

GFP-LRRK2 mutant D2017A transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose at 10 µg

Lane 3:

GFP-LRRK2 mutant S1292A/G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose at 10 µg

Lane 4:

GFP-LRRK2 mutant G2019S transfected HEK293 lysate subjected to immunoprecipitation with GFP trap agarose at 10 µg

Secondary

All lanes:

Goat anti-rabbit (IRDye 800) at 1/10000 dilution

Predicted band size: 286 kDa

Observed band size: 313 kDa

false

Unconjugated

Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8]
Carrier free

Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJFR-19-7-8

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab206035 is the carrier-free version of ab203181.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein LRRK2 also known as leucine-rich repeat kinase 2 or dardarin is an enzyme with a molecular weight of approximately 286 kDa. It functions as a kinase meaning it adds phosphate groups to other proteins which affects their activity. LRRK2 is expressed in various tissues but it is highly abundant in the brain especially in regions such as the striatum and cortex. It has a significant role in cellular signaling processes due to its phosphorylation activity.

Biological function summary

LRRK2 interacts with cellular mechanisms by regulating cytoskeletal dynamics autophagy and vesicle trafficking. It is a part of a larger complex that includes other proteins involved in these processes. The kinase activity of LRRK2 plays an essential part in maintaining neuronal health and function. It influences the process of autophagy which is a way cells clean themselves by removing damaged components and recycling them.

Pathways

The action of LRRK2 is central to the mitogen-activated protein kinase (MAPK) and the mammalian target of rapamycin (mTOR) pathways. In these pathways LRRK2 interacts with other proteins such as mTOR and RPS6KB1. It modulates cellular processes like growth proliferation and response to stressors. Its kinase activity affects the phosphorylation state of targets within the pathways hence influencing biological outcomes like survival and apoptosis.

LRRK2 mutations have a significant connection to Parkinson's disease and Crohn's disease. In Parkinson's disease mutated LRRK2 leads to abnormal protein aggregation linking to proteins such as alpha-synuclein. For Crohn's disease LRRK2 influences the immune response and intestinal inflammation. These connections highlight LRRK2's role in the pathogenesis and contribute to understanding these complex disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene LRRK2 is a serine/threonine-protein kinase that phosphorylates a wide range of proteins involved in neuronal plasticity, autophagy, and vesicle trafficking. It serves as a key regulator of RAB GTPases by affecting the GTP/GDP exchange and interaction partners of RABs through phosphorylation, targeting proteins like RAB3A, RAB3B, and others. LRRK2 manages the GDP/GTP exchange for RAB8A by phosphorylating 'Thr-72', inhibiting RAB8A's interaction with GDI1/GDI2, and influencing primary ciliogenesis to enhance SHH signaling in the brain. It works with RAB29 in retromer-dependent recycling of proteins between lysosomes and the Golgi. The protein is involved in shaping neuronal morphology in the CNS, synaptic vesicle trafficking, and recruiting SEC16A to assist in ER to Golgi transport. It enhances autophagy via the CaMKK/AMPK pathway and relates to nicotinic acid adenine dinucleotide phosphate receptors and lysosomal activity. LRRK2 phosphorylates PRDX3 and APP, influencing neuron apoptosis. Independently, it inhibits MAPT degradation, fostering MAPT oligomerization. Additionally, LRRK2 possesses GTPase activity that regulates its kinase activity. This supplementary information is collated from multiple sources and compiled automatically.

See full target information LRRK2 phospho S1292

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - BSA and Azide free

Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - BSA and Azide free (AB256581)

Western blot - Anti-LRRK2 (phospho S1292) antibody [MJFR-19-7-8] - BSA and Azide free (AB256581)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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