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Rabbit Recombinant Monoclonal LRRK2 phospho S935 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 73 publications.


Images

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (AB133450), expandable thumbnail
  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (AB133450), expandable thumbnail
  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (AB133450), expandable thumbnail
  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (AB133450), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, innate immunity, autophagy, and vesicle trafficking (PubMed:17114044, PubMed:20949042, PubMed:21850687, PubMed:22012985, PubMed:23395371, PubMed:24687852, PubMed:25201882, PubMed:26014385, PubMed:26824392, PubMed:27830463, PubMed:28720718, PubMed:29125462, PubMed:29127255, PubMed:29212815, PubMed:30398148, PubMed:30635421). Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation (PubMed:26824392, PubMed:28720718, PubMed:29125462, PubMed:29127255, PubMed:29212815, PubMed:30398148, PubMed:30635421). Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB29, RAB35, and RAB43 (PubMed:23395371, PubMed:26824392, PubMed:28720718, PubMed:29125462, PubMed:29127255, PubMed:29212815, PubMed:30398148, PubMed:30635421, PubMed:38127736). Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A (PubMed:26824392). Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A (PubMed:26824392). Regulates primary ciliogenesis through phosphorylation of RAB8A and RAB10, which promotes SHH signaling in the brain (PubMed:29125462, PubMed:30398148). Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose-6-phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner (PubMed:23395371). Regulates neuronal process morphology in the intact central nervous system (CNS) (PubMed:17114044). Plays a role in synaptic vesicle trafficking (PubMed:24687852). Plays an important role in recruiting SEC16A to endoplasmic reticulum exit sites (ERES) and in regulating ER to Golgi vesicle-mediated transport and ERES organization (PubMed:25201882). Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway (PubMed:22012985). The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes (PubMed:22012985). Phosphorylates PRDX3 (PubMed:21850687). By phosphorylating APP on 'Thr-743', which promotes the production and the nuclear translocation of the APP intracellular domain (AICD), regulates dopaminergic neuron apoptosis (PubMed:28720718). Acts as a positive regulator of innate immunity by mediating phosphorylation of RIPK2 downstream of NOD1 and NOD2, thereby enhancing RIPK2 activation (PubMed:27830463). Independent of its kinase activity, inhibits the proteasomal degradation of MAPT, thus promoting MAPT oligomerization and secretion (PubMed:26014385). In addition, has GTPase activity via its Roc domain which regulates LRRK2 kinase activity (PubMed:18230735, PubMed:26824392, PubMed:28720718, PubMed:29125462, PubMed:29212815). Recruited by RAB29/RAB7L1 to overloaded lysosomes where it phosphorylates and stabilizes RAB8A and RAB10 which promote lysosomal content release and suppress lysosomal enlargement through the EHBP1 and EHBP1L1 effector proteins (PubMed:30209220, PubMed:38227290).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal LRRK2 phospho S935 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 73 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
UDD2 10(12)
Purification technique
Affinity purification Protein A
Specificity

The antibody does not give a positive signal in U-87 MG, SH-SY-5Y and human fetal brain. Please contact our Scientific Support team if you have any question.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

This antibody was developed with the support of The Michael J. Fox Foundation (MJFF) and in partnership with Dr. Dario Alessi (MRC Protein Phosphorylation Unit, University of Dundee) to help accelerate LRRK2 research. Dr. Alessi has characterized several unique and high quality LRRK2 rabbit monoclonal antibodies, generated by Epitomics, to be made widely available for PD research community.


LRRK2 (Leucine-rich repeat kinase 2, dardarin) is a multi-domain protein belonging to the ROCO family of proteins that contains a kinase and GTPase domain among its many protein interaction domains. LRRK2 is mutated in a significant number of Parkinson's disease (PD) patients. Mutations in this gene account for 4% of PD, and are observed in 1% of sporadic PD patients. The most common mutation replaces glycine 2019 with a serine that results in increased LRRK2 kinase activity. This indicates that inhibitors of LRRK2 kinase activity might be of therapeutic benefit for the treatment of Parkinson's disease and has stimulated much activity in this field of research.
Recent work has revealed that LRRK2 interacts with 14-3-3 phospho-binding adaptor isoforms that is mediated by phosphorylation of Ser910 and Ser935 located prior to the leucine rich repeat domain mediates. Interestingly, 14-3-3 binding has been linked to Parkinson's disease as Ser910 as well as Ser935 and interaction with the 14-3-3 is inhibited by five of the six validated LRRK2 pathogenic mutations (R1441C, R1441G, R1441H, Y1699C and I2020T).
The Dundee-MJFF LRRK2 PhosphoSer935 antibody will be of great utility in further understanding the link between 14-3-3 binding to LRRK2 and Parkinson's disease as well as assessing the efficacy of LRRK2 inhibitors that are being developed. It should be noted the Dundee-MJFF antibody is highly selective and sensitive and can readily be used to monitor LRRK2 Ser935 phosphorylation in immunoblot analysis of 2-20 microgram amounts of whole cell extract. The Dundee-MJFF LRRK2 PhosphoSer935 recognizes both mouse and human endogenous LRRK2.


A BSA and Azide free version of this product is available as Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free ab172382.


Abcam recommended secondaries - Goat Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Or search our wide range of secondary antibodies for use with your experiment.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein LRRK2 also known as leucine-rich repeat kinase 2 or dardarin is an enzyme with a molecular weight of approximately 286 kDa. It functions as a kinase meaning it adds phosphate groups to other proteins which affects their activity. LRRK2 is expressed in various tissues but it is highly abundant in the brain especially in regions such as the striatum and cortex. It has a significant role in cellular signaling processes due to its phosphorylation activity.

Biological function summary

LRRK2 interacts with cellular mechanisms by regulating cytoskeletal dynamics autophagy and vesicle trafficking. It is a part of a larger complex that includes other proteins involved in these processes. The kinase activity of LRRK2 plays an essential part in maintaining neuronal health and function. It influences the process of autophagy which is a way cells clean themselves by removing damaged components and recycling them.

Pathways

The action of LRRK2 is central to the mitogen-activated protein kinase (MAPK) and the mammalian target of rapamycin (mTOR) pathways. In these pathways LRRK2 interacts with other proteins such as mTOR and RPS6KB1. It modulates cellular processes like growth proliferation and response to stressors. Its kinase activity affects the phosphorylation state of targets within the pathways hence influencing biological outcomes like survival and apoptosis.

Associated diseases and disorders

LRRK2 mutations have a significant connection to Parkinson's disease and Crohn's disease. In Parkinson's disease mutated LRRK2 leads to abnormal protein aggregation linking to proteins such as alpha-synuclein. For Crohn's disease LRRK2 influences the immune response and intestinal inflammation. These connections highlight LRRK2's role in the pathogenesis and contribute to understanding these complex disorders.

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4 product images

  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450), expandable thumbnail
    Ito et al PLoS One. 2014 May 16;9(5):e97988. doi: 10.1371/journal.pone.0097988. eCollection 2014. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)

    Inhibitor-induced dephosphorylation of kinase-inactive LRRK2.

    HEK-293 cells transfected with wild-type, or mutated LRRK2 (K1906A, K1906M, D1994A, D1994N, D2017A, S2032A or T2035A) were treated with 3 μM LRRK2-IN-1 (LRRK2 inhibitor) for 30 min. The phosphorylation of LRRK2 at Ser910, Ser935, or Ser955 was examined by immunoblotting. This showed that the responses of LRRK2 to the inhibitor varied among mutants.

    LRRK2 (phospho S935) was detected using Anti-LRRK2 (phospho S910) antibody [UDD1 15(3)] ab133449.

    LRRK2 (phospho S935) was detected using ab133450.

    LRRK2 (phospho S955) was detected using Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)] ab169521.

    All lanes: Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)

    Predicted band size: 286 kDa

  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450), expandable thumbnail

    Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)

    All lanes: Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/1000 dilution

    Lane 1: GFP LRRK2 lysate at 5 µg

    Lane 2: GFP LRRK2 S910A lysate at 5 µg

    Lane 3: GFP LRRK2 S935A lysate at 5 µg

    Lane 4: LRRK2 WT MEF lysate at 20 µg

    Lane 5: LRRK2 WT MEF lysate from LRRK2 IN1 treated cells at 20 µg

    Lane 6: LRRK2 KO MEF lysate at 20 µg

    Lane 7: LRRK2 KO MEF lysate from LRRK2 IN1 treated cells at 20 µg

    Lane 8: Lymphoblastoid lysate at 30 µg

    Lane 9: Lymphoblastoid lysate from LRRK2 IN1 treated cells at 30 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 286 kDa

  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450), expandable thumbnail

    Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/5000 dilution

    Lane 1: WT-LRRK2 cell lysate - untreated at 10 µg

    Lane 2: WT-LRRK2 cell lysate - treated with Lambda phosphatase at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 286 kDa, 39 kDa

    Observed band size: 286 kDa, 39 kDa

  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450), expandable thumbnail

    Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/1000 dilution

    All lanes: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 286 kDa

    Observed band size: 286 kDa

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