AB172382

Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(14 Publications)

Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting LRRK2 in Western Blot. Suitable for Human, Mouse.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

PARK8, LRRK2, Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin

3 Images

Unknown

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (AB172382)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133450).

All lanes:

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (<a href='/en-us/products/primary-antibodies/lrrk2-phospho-s935-antibody-udd2-1012-ab133450'>ab133450</a>) at 1/1000 dilution

Lane 1:

GFP LRRK2 lysate at 5 µg

Lane 2:

GFP LRRK2 S910A lysate at 5 µg

Lane 3:

GFP LRRK2 S935A lysate at 5 µg

Lane 4:

LRRK2 WT MEF lysate at 20 µg

Lane 5:

LRRK2 WT MEF lysate from LRRK2 IN1 treated cells at 20 µg

Lane 6:

LRRK2 KO MEF lysate at 20 µg

Lane 7:

LRRK2 KO MEF lysate from LRRK2 IN1 treated cells at 20 µg

Lane 8:

Lymphoblastoid lysate at 30 µg

Lane 9:

Lymphoblastoid lysate from LRRK2 IN1 treated cells at 30 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 286 kDa

false

Lab

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (AB172382)

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133450).

All lanes:

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (<a href='/en-us/products/primary-antibodies/lrrk2-phospho-s935-antibody-udd2-1012-ab133450'>ab133450</a>) at 1/5000 dilution

Lane 1:

WT-LRRK2 cell lysate - untreated at 10 µg

Lane 2:

WT-LRRK2 cell lysate - treated with Lambda phosphatase at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 286 kDa,39 kDa

Observed band size: 286 kDa,39 kDa

false

Lab

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (AB172382)

Exposure :
Lane 1 and 2 : 5 seconds.
Lane 3 : 180 seconds.

All lanes:

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382) at 1/10000 dilution

Lane 1:

Wide type-LRRK2 overexpressed lysates at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

Lane 3:

RAW 264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage)whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 286 kDa

false

Unconjugated

Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)]
Carrier free

Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

UDD2 10(12)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody does not give a positive signal in U-87 MG, SH-SY-5Y and human fetal brain. Please contact our Scientific Support team if you have any question.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Please check the parent abID, <a href='/en-us/products/primary-antibodies/lrrk2-phospho-s935-antibody-udd2-1012-ab133450'>ab133450</a>, for a recommended dilution.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Please check the parent abID, <a href='/en-us/products/primary-antibodies/lrrk2-phospho-s935-antibody-udd2-1012-ab133450'>ab133450</a>, for a recommended dilution.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Please check the parent abID, <a href='/en-us/products/primary-antibodies/lrrk2-phospho-s935-antibody-udd2-1012-ab133450'>ab133450</a>, for a recommended dilution.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.

What is the molecular weight of LRRK2?
Anti-LRRK2 (phospho S935) [UDD2 10(12)] - BSA and Azide free (ab172382) specifically detects a band for LRRK2 (UniProt: Q5S007) at a molecular weight of 286kDa.

Trusted by the scientific community
Anti-LRRK2 (phospho S935) [UDD2 10(12)] - BSA and Azide free (ab172382) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [UDD2 10(12)] also available for your convenience: ab133450, Carrier free - ab172382, Carrier free - ab324096

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein LRRK2 also known as leucine-rich repeat kinase 2 or dardarin is an enzyme with a molecular weight of approximately 286 kDa. It functions as a kinase meaning it adds phosphate groups to other proteins which affects their activity. LRRK2 is expressed in various tissues but it is highly abundant in the brain especially in regions such as the striatum and cortex. It has a significant role in cellular signaling processes due to its phosphorylation activity.

Biological function summary

LRRK2 interacts with cellular mechanisms by regulating cytoskeletal dynamics autophagy and vesicle trafficking. It is a part of a larger complex that includes other proteins involved in these processes. The kinase activity of LRRK2 plays an essential part in maintaining neuronal health and function. It influences the process of autophagy which is a way cells clean themselves by removing damaged components and recycling them.

Pathways

The action of LRRK2 is central to the mitogen-activated protein kinase (MAPK) and the mammalian target of rapamycin (mTOR) pathways. In these pathways LRRK2 interacts with other proteins such as mTOR and RPS6KB1. It modulates cellular processes like growth proliferation and response to stressors. Its kinase activity affects the phosphorylation state of targets within the pathways hence influencing biological outcomes like survival and apoptosis.

LRRK2 mutations have a significant connection to Parkinson's disease and Crohn's disease. In Parkinson's disease mutated LRRK2 leads to abnormal protein aggregation linking to proteins such as alpha-synuclein. For Crohn's disease LRRK2 influences the immune response and intestinal inflammation. These connections highlight LRRK2's role in the pathogenesis and contribute to understanding these complex disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, innate immunity, autophagy, and vesicle trafficking (PubMed : 17114044, PubMed : 20949042, PubMed : 21850687, PubMed : 22012985, PubMed : 23395371, PubMed : 24687852, PubMed : 25201882, PubMed : 26014385, PubMed : 26824392, PubMed : 27830463, PubMed : 28720718, PubMed : 29125462, PubMed : 29127255, PubMed : 29212815, PubMed : 30398148, PubMed : 30635421). Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation (PubMed : 26824392, PubMed : 28720718, PubMed : 29125462, PubMed : 29127255, PubMed : 29212815, PubMed : 30398148, PubMed : 30635421). Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB29, RAB35, and RAB43 (PubMed : 23395371, PubMed : 26824392, PubMed : 28720718, PubMed : 29125462, PubMed : 29127255, PubMed : 29212815, PubMed : 30398148, PubMed : 30635421, PubMed : 38127736). Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A (PubMed : 26824392). Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A (PubMed : 26824392). Regulates primary ciliogenesis through phosphorylation of RAB8A and RAB10, which promotes SHH signaling in the brain (PubMed : 29125462, PubMed : 30398148). Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose-6-phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner (PubMed : 23395371). Regulates neuronal process morphology in the intact central nervous system (CNS) (PubMed : 17114044). Plays a role in synaptic vesicle trafficking (PubMed : 24687852). Plays an important role in recruiting SEC16A to endoplasmic reticulum exit sites (ERES) and in regulating ER to Golgi vesicle-mediated transport and ERES organization (PubMed : 25201882). Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway (PubMed : 22012985). The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes (PubMed : 22012985). Phosphorylates PRDX3 (PubMed : 21850687). By phosphorylating APP on 'Thr-743', which promotes the production and the nuclear translocation of the APP intracellular domain (AICD), regulates dopaminergic neuron apoptosis (PubMed : 28720718). Acts as a positive regulator of innate immunity by mediating phosphorylation of RIPK2 downstream of NOD1 and NOD2, thereby enhancing RIPK2 activation (PubMed : 27830463). Independent of its kinase activity, inhibits the proteasomal degradation of MAPT, thus promoting MAPT oligomerization and secretion (PubMed : 26014385). In addition, has GTPase activity via its Roc domain which regulates LRRK2 kinase activity (PubMed : 18230735, PubMed : 26824392, PubMed : 28720718, PubMed : 29125462, PubMed : 29212815). Recruited by RAB29/RAB7L1 to overloaded lysosomes where it phosphorylates and stabilizes RAB8A and RAB10 which promote lysosomal content release and suppress lysosomal enlargement through the EHBP1 and EHBP1L1 effector proteins (PubMed : 30209220, PubMed : 38227290).

See full target information LRRK2 phospho S935

style

left-column

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 9:44 PubMed36973269

2023

The small GTPase Rit2 modulates LRRK2 kinase activity, is required for lysosomal function and protects against alpha-synuclein neuropathology.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Obergasteiger,Anne-Marie Castonguay,Sara Pizzi,Stefano Magnabosco,Giulia Frapporti,Evy Lobbestael,Veerle Baekelandt,Andrew A Hicks,Peter P Pramstaller,Claude Gravel,Corrado Corti,Martin Lévesque,Mattia Volta

NPJ Parkinson's disease 8:92 PubMed35853899

2022

LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Kedariti,Emanuele Frattini,Pascale Baden,Susanna Cogo,Laura Civiero,Elena Ziviani,Gianluca Zilio,Federico Bertoli,Massimo Aureli,Alice Kaganovich,Mark R Cookson,Leonidas Stefanis,Matthew Surface,Michela Deleidi,Alessio Di Fonzo,Roy N Alcalay,Hardy Rideout,Elisa Greggio,Nicoletta Plotegher

Cells 9: PubMed33105882

2020

Divergent Effects of G2019S and R1441C LRRK2 Mutations on LRRK2 and Rab10 Phosphorylations in Mouse Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Lucia Iannotta,Alice Biosa,Jillian H Kluss,Giulia Tombesi,Alice Kaganovich,Susanna Cogo,Nicoletta Plotegher,Laura Civiero,Evy Lobbestael,Veerle Baekelandt,Mark R Cookson,Elisa Greggio

Cell death discovery 6:45 PubMed32550012

2020

Kinase inhibition of G2019S-LRRK2 enhances autolysosome formation and function to reduce endogenous alpha-synuclein intracellular inclusions.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Obergasteiger,Giulia Frapporti,Giulia Lamonaca,Sara Pizzi,Anne Picard,Alexandros A Lavdas,Francesca Pischedda,Giovanni Piccoli,Sabine Hilfiker,Evy Lobbestael,Veerle Baekelandt,Andrew A Hicks,Corrado Corti,Peter P Pramstaller,Mattia Volta

Nature communications 9:3465 PubMed30150626

2018

LRRK2 kinase regulates α-synuclein propagation via RAB35 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Jin Bae,Dong-Kyu Kim,Changyoun Kim,Michael Mante,Anthony Adame,Edward Rockenstein,Ayse Ulusoy,Michael Klinkenberg,Ga Ram Jeong,Jae Ryul Bae,Cheolsoon Lee,He-Jin Lee,Byung-Dae Lee,Donato A Di Monte,Eliezer Masliah,Seung-Jae Lee

Experimental neurobiology 27:171-180 PubMed30022868

2018

LRRK2 Kinase Activity Induces Mitochondrial Fission in Microglia via Drp1 and Modulates Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Hwan Ho,A Reum Je,Haejin Lee,Ilhong Son,Hee-Seok Kweon,Hyung-Gun Kim,Wongi Seol

Acta neuropathologica communications 6:45 PubMed29855356

2018

LRRK2 activity does not dramatically alter α-synuclein pathology in primary neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Michael X Henderson,Chao Peng,John Q Trojanowski,Virginia M Y Lee

Bioscience reports 38: PubMed29563162

2018

mTOR independent alteration in ULK1 Ser758 phosphorylation following chronic LRRK2 kinase inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Manzoni,Adamantios Mamais,Sybille Dihanich,Marc P M Soutar,Helene Plun-Favreau,Rina Bandopadhyay,Rosella Abeti,Paola Giunti,John Hardy,Mark R Cookson,Sharon A Tooze,Patrick A Lewis

Molecular neurodegeneration 13:3 PubMed29357897

2018

Parkinson disease-associated mutations in LRRK2 cause centrosomal defects via Rab8a phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jesús Madero-Pérez,Elena Fdez,Belén Fernández,Antonio J Lara Ordóñez,Marian Blanca Ramírez,Patricia Gómez-Suaga,Dieter Waschbüsch,Evy Lobbestael,Veerle Baekelandt,Angus C Nairn,Javier Ruiz-Martínez,Ana Aiastui,Adolfo López de Munain,Pawel Lis,Thomas Comptdaer,Jean-Marc Taymans,Marie-Christine Chartier-Harlin,Alexandria Beilina,Adriano Gonnelli,Mark R Cookson,Elisa Greggio,Sabine Hilfiker

Toxicology 395:15-22 PubMed29307545

2018

PFE-360-induced LRRK2 inhibition induces reversible, non-adverse renal changes in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Aagaard Andersen,Karen Malene Wegener,Steen Larsen,Lassina Badolo,Garrick Paul Smith,Ross Jeggo,Poul Henning Jensen,Florence Sotty,Kenneth Vielsted Christensen,Annemette Thougaard

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (AB172382)

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (AB172382)

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (AB172382)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise