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Anti-LRRK2 (phospho S935) [UDD2 10(12)] antibody (ab172382) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect LRRK2 in Western Blot. Suitable for Human, Mouse samples.



- BSA,?sodium azide, and glycerol-free?for consistent conjugation with fluorochromes, enzymes and more

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Images

Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (AB172382), expandable thumbnail
  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (AB172382), expandable thumbnail
  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (AB172382), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Transfected cell line
Tested
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Please check the parent abID, Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450, for a recommended dilution.

Species
Human
Dilution info
-
Notes

Please check the parent abID, Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450, for a recommended dilution.

Species
Transfected cell line
Dilution info
-
Notes

Please check the parent abID, Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450, for a recommended dilution.

Not recommended
Not recommended

Species
Mouse, Human, Transfected cell line
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Transfected cell line
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

The protein expressed by the gene LRRK2 is a serine/threonine-protein kinase that phosphorylates a wide range of proteins involved in neuronal plasticity, autophagy, and vesicle trafficking. It serves as a key regulator of RAB GTPases by affecting the GTP/GDP exchange and interaction partners of RABs through phosphorylation, targeting proteins like RAB3A, RAB3B, and others. LRRK2 manages the GDP/GTP exchange for RAB8A by phosphorylating 'Thr-72', inhibiting RAB8A's interaction with GDI1/GDI2, and influencing primary ciliogenesis to enhance SHH signaling in the brain. It works with RAB29 in retromer-dependent recycling of proteins between lysosomes and the Golgi. The protein is involved in shaping neuronal morphology in the CNS, synaptic vesicle trafficking, and recruiting SEC16A to assist in ER to Golgi transport. It enhances autophagy via the CaMKK/AMPK pathway and relates to nicotinic acid adenine dinucleotide phosphate receptors and lysosomal activity. LRRK2 phosphorylates PRDX3 and APP, influencing neuron apoptosis. Independently, it inhibits MAPT degradation, fostering MAPT oligomerization. Additionally, LRRK2 possesses GTPase activity that regulates its kinase activity. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Recommended products

Anti-LRRK2 (phospho S935) [UDD2 10(12)] antibody (ab172382) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect LRRK2 in Western Blot. Suitable for Human, Mouse samples.



- BSA,?sodium azide, and glycerol-free?for consistent conjugation with fluorochromes, enzymes and more

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
UDD2 10(12)
Purification technique
Affinity purification Protein A
Specificity

The antibody does not give a positive signal in U-87 MG, SH-SY-5Y and human fetal brain. Please contact our Scientific Support team if you have any question.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab172382 is the carrier-free version of Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein LRRK2 also known as leucine-rich repeat kinase 2 or dardarin is an enzyme with a molecular weight of approximately 286 kDa. It functions as a kinase meaning it adds phosphate groups to other proteins which affects their activity. LRRK2 is expressed in various tissues but it is highly abundant in the brain especially in regions such as the striatum and cortex. It has a significant role in cellular signaling processes due to its phosphorylation activity.

Biological function summary

LRRK2 interacts with cellular mechanisms by regulating cytoskeletal dynamics autophagy and vesicle trafficking. It is a part of a larger complex that includes other proteins involved in these processes. The kinase activity of LRRK2 plays an essential part in maintaining neuronal health and function. It influences the process of autophagy which is a way cells clean themselves by removing damaged components and recycling them.

Pathways

The action of LRRK2 is central to the mitogen-activated protein kinase (MAPK) and the mammalian target of rapamycin (mTOR) pathways. In these pathways LRRK2 interacts with other proteins such as mTOR and RPS6KB1. It modulates cellular processes like growth proliferation and response to stressors. Its kinase activity affects the phosphorylation state of targets within the pathways hence influencing biological outcomes like survival and apoptosis.

Associated diseases and disorders

LRRK2 mutations have a significant connection to Parkinson's disease and Crohn's disease. In Parkinson's disease mutated LRRK2 leads to abnormal protein aggregation linking to proteins such as alpha-synuclein. For Crohn's disease LRRK2 influences the immune response and intestinal inflammation. These connections highlight LRRK2's role in the pathogenesis and contribute to understanding these complex disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382), expandable thumbnail

    Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450).

    All lanes: Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450) at 1/1000 dilution

    Lane 1: GFP LRRK2 lysate at 5 µg

    Lane 2: GFP LRRK2 S910A lysate at 5 µg

    Lane 3: GFP LRRK2 S935A lysate at 5 µg

    Lane 4: LRRK2 WT MEF lysate at 20 µg

    Lane 5: LRRK2 WT MEF lysate from LRRK2 IN1 treated cells at 20 µg

    Lane 6: LRRK2 KO MEF lysate at 20 µg

    Lane 7: LRRK2 KO MEF lysate from LRRK2 IN1 treated cells at 20 µg

    Lane 8: Lymphoblastoid lysate at 30 µg

    Lane 9: Lymphoblastoid lysate from LRRK2 IN1 treated cells at 30 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 286 kDa

  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382), expandable thumbnail

    Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)

    Exposure:
    Lane 1 and 2: 5 seconds.
    Lane 3: 180 seconds.

    All lanes: Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382) at 1/10000 dilution

    Lane 1: Wide type-LRRK2 overexpressed lysates at 15 µg

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg

    Lane 3: RAW 264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage)whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 286 kDa

  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382), expandable thumbnail

    Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450).

    All lanes: Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450) at 1/5000 dilution

    Lane 1: WT-LRRK2 cell lysate - untreated at 10 µg

    Lane 2: WT-LRRK2 cell lysate - treated with Lambda phosphatase at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 286 kDa, 39 kDa

    Observed band size: 286 kDa, 39 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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