Anti-LRRK2 (phospho S935) [UDD2 10(12)] antibody (ab172382) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect LRRK2 in Western Blot. Suitable for Human, Mouse samples.
- BSA,?sodium azide, and glycerol-free?for consistent conjugation with fluorochromes, enzymes and more
Constituents: PBS
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Not recommended | Not recommended |
Mouse | Tested | Not recommended | Not recommended |
Transfected cell line | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Please check the parent abID, Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450, for a recommended dilution. |
Species Human | Dilution info - | Notes Please check the parent abID, Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450, for a recommended dilution. |
Species Transfected cell line | Dilution info - | Notes Please check the parent abID, Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450, for a recommended dilution. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Transfected cell line | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Transfected cell line | Dilution info - | Notes - |
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The protein expressed by the gene LRRK2 is a serine/threonine-protein kinase that phosphorylates a wide range of proteins involved in neuronal plasticity, autophagy, and vesicle trafficking. It serves as a key regulator of RAB GTPases by affecting the GTP/GDP exchange and interaction partners of RABs through phosphorylation, targeting proteins like RAB3A, RAB3B, and others. LRRK2 manages the GDP/GTP exchange for RAB8A by phosphorylating 'Thr-72', inhibiting RAB8A's interaction with GDI1/GDI2, and influencing primary ciliogenesis to enhance SHH signaling in the brain. It works with RAB29 in retromer-dependent recycling of proteins between lysosomes and the Golgi. The protein is involved in shaping neuronal morphology in the CNS, synaptic vesicle trafficking, and recruiting SEC16A to assist in ER to Golgi transport. It enhances autophagy via the CaMKK/AMPK pathway and relates to nicotinic acid adenine dinucleotide phosphate receptors and lysosomal activity. LRRK2 phosphorylates PRDX3 and APP, influencing neuron apoptosis. Independently, it inhibits MAPT degradation, fostering MAPT oligomerization. Additionally, LRRK2 possesses GTPase activity that regulates its kinase activity. This supplementary information is collated from multiple sources and compiled automatically.
PARK8, LRRK2, Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin
Anti-LRRK2 (phospho S935) [UDD2 10(12)] antibody (ab172382) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect LRRK2 in Western Blot. Suitable for Human, Mouse samples.
- BSA,?sodium azide, and glycerol-free?for consistent conjugation with fluorochromes, enzymes and more
Constituents: PBS
The antibody does not give a positive signal in U-87 MG, SH-SY-5Y and human fetal brain. Please contact our Scientific Support team if you have any question.
ab172382 is the carrier-free version of Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.
The protein LRRK2 also known as leucine-rich repeat kinase 2 or dardarin is an enzyme with a molecular weight of approximately 286 kDa. It functions as a kinase meaning it adds phosphate groups to other proteins which affects their activity. LRRK2 is expressed in various tissues but it is highly abundant in the brain especially in regions such as the striatum and cortex. It has a significant role in cellular signaling processes due to its phosphorylation activity.
LRRK2 interacts with cellular mechanisms by regulating cytoskeletal dynamics autophagy and vesicle trafficking. It is a part of a larger complex that includes other proteins involved in these processes. The kinase activity of LRRK2 plays an essential part in maintaining neuronal health and function. It influences the process of autophagy which is a way cells clean themselves by removing damaged components and recycling them.
The action of LRRK2 is central to the mitogen-activated protein kinase (MAPK) and the mammalian target of rapamycin (mTOR) pathways. In these pathways LRRK2 interacts with other proteins such as mTOR and RPS6KB1. It modulates cellular processes like growth proliferation and response to stressors. Its kinase activity affects the phosphorylation state of targets within the pathways hence influencing biological outcomes like survival and apoptosis.
LRRK2 mutations have a significant connection to Parkinson's disease and Crohn's disease. In Parkinson's disease mutated LRRK2 leads to abnormal protein aggregation linking to proteins such as alpha-synuclein. For Crohn's disease LRRK2 influences the immune response and intestinal inflammation. These connections highlight LRRK2's role in the pathogenesis and contribute to understanding these complex disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450).
All lanes: Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450) at 1/1000 dilution
Lane 1: GFP LRRK2 lysate at 5 µg
Lane 2: GFP LRRK2 S910A lysate at 5 µg
Lane 3: GFP LRRK2 S935A lysate at 5 µg
Lane 4: LRRK2 WT MEF lysate at 20 µg
Lane 5: LRRK2 WT MEF lysate from LRRK2 IN1 treated cells at 20 µg
Lane 6: LRRK2 KO MEF lysate at 20 µg
Lane 7: LRRK2 KO MEF lysate from LRRK2 IN1 treated cells at 20 µg
Lane 8: Lymphoblastoid lysate at 30 µg
Lane 9: Lymphoblastoid lysate from LRRK2 IN1 treated cells at 30 µg
All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 286 kDa
All lanes: Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382) at 1/10000 dilution
Lane 1: Wide type-LRRK2 overexpressed lysates at 15 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg
Lane 3: RAW 264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage)whole cell lysates at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 286 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450).
All lanes: Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] ab133450) at 1/5000 dilution
Lane 1: WT-LRRK2 cell lysate - untreated at 10 µg
Lane 2: WT-LRRK2 cell lysate - treated with Lambda phosphatase at 10 µg
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 286 kDa, 39 kDa
Observed band size: 286 kDa, 39 kDa
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