AB156577

Anti-LRRK2 (phospho T2483) antibody [MJF-R8 (21-2e)]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal LRRK2 phospho T2483 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

PARK8, LRRK2, Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin

1 Images

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Western blot - Anti-LRRK2 (phospho T2483) antibody [MJF-R8 (21-2e)] (AB156577)

LRRK2 protein was immunoprecipitated and subjected to an in vitro kinase assay in the presence (+) or absence (-) of ATP.

All lanes:

Western blot - Anti-LRRK2 (phospho T2483) antibody [MJF-R8 (21-2e)] (ab156577) at 1/1000 dilution

Lane 1:

HEK293 cells transiently transfected with wild type LRRK2 at 10 µg

Lane 2:

HEK293 cells transiently transfected with T2483 at 10 µg

Lane 3:

HEK293 cells transiently transfected with G2019S at 10 µg

Lane 4:

HEK293 cells transiently transfected with Kinase dead at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 286 kDa

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Carrier free

Anti-LRRK2 (phospho T2483) antibody [MJF-R8 (21-2e)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-R8 (21-2e)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein LRRK2 also known as leucine-rich repeat kinase 2 or dardarin is an enzyme with a molecular weight of approximately 286 kDa. It functions as a kinase meaning it adds phosphate groups to other proteins which affects their activity. LRRK2 is expressed in various tissues but it is highly abundant in the brain especially in regions such as the striatum and cortex. It has a significant role in cellular signaling processes due to its phosphorylation activity.

Biological function summary

LRRK2 interacts with cellular mechanisms by regulating cytoskeletal dynamics autophagy and vesicle trafficking. It is a part of a larger complex that includes other proteins involved in these processes. The kinase activity of LRRK2 plays an essential part in maintaining neuronal health and function. It influences the process of autophagy which is a way cells clean themselves by removing damaged components and recycling them.

Pathways

The action of LRRK2 is central to the mitogen-activated protein kinase (MAPK) and the mammalian target of rapamycin (mTOR) pathways. In these pathways LRRK2 interacts with other proteins such as mTOR and RPS6KB1. It modulates cellular processes like growth proliferation and response to stressors. Its kinase activity affects the phosphorylation state of targets within the pathways hence influencing biological outcomes like survival and apoptosis.

LRRK2 mutations have a significant connection to Parkinson's disease and Crohn's disease. In Parkinson's disease mutated LRRK2 leads to abnormal protein aggregation linking to proteins such as alpha-synuclein. For Crohn's disease LRRK2 influences the immune response and intestinal inflammation. These connections highlight LRRK2's role in the pathogenesis and contribute to understanding these complex disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene LRRK2 is a serine/threonine-protein kinase that phosphorylates a wide range of proteins involved in neuronal plasticity, autophagy, and vesicle trafficking. It serves as a key regulator of RAB GTPases by affecting the GTP/GDP exchange and interaction partners of RABs through phosphorylation, targeting proteins like RAB3A, RAB3B, and others. LRRK2 manages the GDP/GTP exchange for RAB8A by phosphorylating 'Thr-72', inhibiting RAB8A's interaction with GDI1/GDI2, and influencing primary ciliogenesis to enhance SHH signaling in the brain. It works with RAB29 in retromer-dependent recycling of proteins between lysosomes and the Golgi. The protein is involved in shaping neuronal morphology in the CNS, synaptic vesicle trafficking, and recruiting SEC16A to assist in ER to Golgi transport. It enhances autophagy via the CaMKK/AMPK pathway and relates to nicotinic acid adenine dinucleotide phosphate receptors and lysosomal activity. LRRK2 phosphorylates PRDX3 and APP, influencing neuron apoptosis. Independently, it inhibits MAPT degradation, fostering MAPT oligomerization. Additionally, LRRK2 possesses GTPase activity that regulates its kinase activity. This supplementary information is collated from multiple sources and compiled automatically.

See full target information LRRK2 phospho T2483

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of 116:14979-14988 PubMed31292254

2019

The dynamic switch mechanism that leads to activation of LRRK2 is embedded in the DFGψ motif in the kinase domain.

Applications

Unspecified application

Species

Unspecified reactive species

Sven H Schmidt,Matthias J Knape,Daniela Boassa,Natascha Mumdey,Alexandr P Kornev,Mark H Ellisman,Susan S Taylor,Friedrich W Herberg

Journal of neurochemistry 135:1242-56 PubMed26375402

2015

Leucine-rich repeat kinase 2 interacts with p21-activated kinase 6 to control neurite complexity in mammalian brain.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Civiero,Maria Daniela Cirnaru,Alexandra Beilina,Umberto Rodella,Isabella Russo,Elisa Belluzzi,Evy Lobbestael,Lauran Reyniers,Geshanthi Hondhamuni,Patrick A Lewis,Chris Van den Haute,Veerle Baekelandt,Rina Bandopadhyay,Luigi Bubacco,Giovanni Piccoli,Mark R Cookson,Jean-Marc Taymans,Elisa Greggio

Proceedings of the National Academy of Sciences of the United States of America 111:E34-43 PubMed24351927

2013

Parkinson-related LRRK2 mutation R1441C/G/H impairs PKA phosphorylation of LRRK2 and disrupts its interaction with 14-3-3.

Applications

Species

Unspecified reactive species

Kathrin Muda,Daniela Bertinetti,Frank Gesellchen,Jennifer Sarah Hermann,Felix von Zweydorf,Arie Geerlof,Anette Jacob,Marius Ueffing,Christian Johannes Gloeckner,Friedrich W Herberg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LRRK2 (phospho T2483) antibody [MJF-R8 (21-2e)]

Western blot - Anti-LRRK2 (phospho T2483) antibody [MJF-R8 (21-2e)] (AB156577)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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