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AB73113

Anti-LRSAM1 antibody

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(8 Publications)

Mouse Polyclonal LRSAM1 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LRSAM1.

View Alternative Names

TAL, UNQ6496/PRO21356, LRSAM1, E3 ubiquitin-protein ligase LRSAM1, Leucine-rich repeat and sterile alpha motif-containing protein 1, RING-type E3 ubiquitin transferase LRSAM1, Tsg101-associated ligase, hTAL

2 Images
Western blot - Anti-LRSAM1 antibody (AB73113)
  • WB

Unknown

Western blot - Anti-LRSAM1 antibody (AB73113)

All lanes:

Western blot - Anti-LRSAM1 antibody (ab73113) at 1/500 dilution

All lanes:

Human liver tissue lysate at 25 µg

Secondary

All lanes:

HRP-conjugated Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

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Western blot - Anti-LRSAM1 antibody (AB73113)
  • WB

Unknown

Western blot - Anti-LRSAM1 antibody (AB73113)

All lanes:

Western blot - Anti-LRSAM1 antibody (ab73113) at 1/500 dilution

Lane 1:

LRSAM1-transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LRSAM1.

Q6UWE0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LRSAM1 also known as Leucine-rich repeat and Sterile Alpha Motif-containing protein 1 functions mechanically as an E3 ubiquitin ligase. It plays an important role in tagging proteins for degradation. The protein mass of LRSAM1 is approximately 82 kDa. This protein expresses across various tissues with notable presence in the central nervous system including neurons. LRSAM1 also interacts within the cytoplasm and nucleus influencing multiple cellular processes.
Biological function summary

LRSAM1 contributes critically to the regulation of protein homeostasis. It does not form a known large multimeric complex but may interact closely with other components in the ubiquitination machinery. This process is significant for cellular control of protein turnover affecting cellular signaling and response to stress. LRSAM1 assists in maintaining protein quality by marking damaged or unnecessary proteins for destruction.

Pathways

LRSAM1 regulates protein turnover through its role in the ubiquitin-proteasome system. It influences pathways related to protein quality control and signal transduction. In these pathways LRSAM1 shows interactions with proteins such as ubiquitin and proteasomal subunits. This connects LRSAM1 to cellular mechanisms that monitor and regulate cell cycle apoptosis and response to oxidative stress.

Mutations or dysregulation of LRSAM1 relate to Charcot-Marie-Tooth disease type 2P and some forms of neurodegenerative disorders. Charcot-Marie-Tooth disease type 2P stems from mutations in LRSAM1 affecting peripheral nerve function. The protein's involvement with disorders like these highlights its connection to cellular stress responses. LRSAM1 links to proteins involved in mitochondrial function emphasizing its role in maintaining neural health and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos (PubMed : 15256501). Bacterial recognition protein that defends the cytoplasm from invasive pathogens (PubMed : 23245322). Localizes to several intracellular bacterial pathogens and generates the bacteria-associated ubiquitin signal leading to autophagy-mediated intracellular bacteria degradation (xenophagy) (PubMed : 23245322, PubMed : 25484098).
See full target information LRSAM1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

PloS one 14:e0211814 PubMed30726272

2019

Deregulation of LRSAM1 expression impairs the levels of TSG101, UBE2N, VPS28, MDM2 and EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Minaidou,Paschalis Nicolaou,Kyproula Christodoulou

Cell journal 20:340-347 PubMed29845787

2018

LRSAM1 Depletion Affects Neuroblastoma SH-SY5Y Cell Growth and Morphology: The LRSAM1 c.2047-1G>A Loss-of-Function Variant Fails to Rescue The Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Minaidou,Paschalis Nicolaou,Kyproula Christodoulou

EMBO reports 18:1572-1585 PubMed28784601

2017

Expanding the host cell ubiquitylation machinery targeting cytosolic .

Applications

Unspecified application

Species

Unspecified reactive species

Mira Polajnar,Marina S Dietz,Mike Heilemann,Christian Behrends

Annals of neurology 80:823-833 PubMed27686364

2016

Charcot-Marie-Tooth disease type 2G redefined by a novel mutation in LRSAM1.

Applications

Unspecified application

Species

Unspecified reactive species

Kristien Peeters,Paulius Palaima,Ana L Pelayo-Negro,Antonio García,Elena Gallardo,Rosario García-Barredo,Ligia Mateiu,Jonathan Baets,Björn Menten,Els De Vriendt,Peter De Jonghe,Vincent Timmerman,Jon Infante,José Berciano,Albena Jordanova

Annals of neurology 80:834-845 PubMed27615052

2016

A novel missense mutation of CMT2P alters transcription machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hu,Sezgi Arpag,Stephan Zuchner,Jun Li

Disease models & mechanisms 6:780-92 PubMed23519028

2013

Loss of the E3 ubiquitin ligase LRSAM1 sensitizes peripheral axons to degeneration in a mouse model of Charcot-Marie-Tooth disease.

Applications

WB

Species

Unspecified reactive species

Laurent P Bogdanik,James N Sleigh,Cong Tian,Mark E Samuels,Karen Bedard,Kevin L Seburn,Robert W Burgess

European journal of human genetics : EJHG 21:190-4 PubMed22781092

2012

A novel LRSAM1 mutation is associated with autosomal dominant axonal Charcot-Marie-Tooth disease.

Applications

Unspecified application

Species

Human

Paschalis Nicolaou,Carlo Cianchetti,Anna Minaidou,Giovanni Marrosu,Eleni Zamba-Papanicolaou,Lefkos Middleton,Kyproula Christodoulou

PLoS genetics 6: PubMed20865121

2010

Mutation in the gene encoding ubiquitin ligase LRSAM1 in patients with Charcot-Marie-Tooth disease.

Applications

WB

Species

Human

Duane L Guernsey,Haiyan Jiang,Karen Bedard,Susan C Evans,Meghan Ferguson,Makoto Matsuoka,Christine Macgillivray,Mathew Nightingale,Scott Perry,Andrea L Rideout,Andrew Orr,Mark Ludman,David L Skidmore,Timothy Benstead,Mark E Samuels
View all publications
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