Rabbit Polyclonal LRWD1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LRWD1 aa 100-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LRWD1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UFC0
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.
Alternative names
CENP-33, ORCA, LRWD1, Leucine-rich repeat and WD repeat-containing protein 1, Centromere protein 33, Origin recognition complex-associated protein, ORC-associated protein
Recommended products
Rabbit Polyclonal LRWD1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LRWD1 aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LRWD1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UFC0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The LRWD1 also known as Leucine-rich repeats and WD repeat-containing protein 1 plays an essential mechanical role in cells. This protein weighs approximately 63 kDa and is expressed mainly in tissues such as the testis. LRWD1 has structural domains that include leucine-rich repeats and WD repeat domains contributing to its role in protein interactions and signaling pathways.
- Biological function summary
LRWD1 supports the regulation of DNA replication and centrosome cycle. It is an integral part of the DNA replication licensing pathway ensuring proper DNA synthesis during the cell cycle. By participating in this complex LRWD1 collaborates with proteins like CDC6 and CDT1 to initiate DNA replication. This protein ensures that cells duplicate their DNA once per cycle maintaining genomic stability.
- Pathways
LRWD1 participates in the DNA replication pathway and is also linked to the G2 to M phase transition of the cell cycle. Through the DNA replication pathway LRWD1 works hand-in-hand with other factors such as ORC1 and MCM proteins which coordinate the licensing of replication origins. Its presence in these pathways demonstrates its pivotal role in maintaining normal cell cycle progression and genomic integrity.
- Associated diseases and disorders
LRWD1 has associations with disorders involving improper DNA replication and genome instability. Misregulation of this protein can relate to conditions such as cancer due to its involvement in cell cycle control. In cancer LRWD1's interaction with DNA replication-related proteins highlights the pathway's relevance in tumorigenesis. Researchers continue to investigate its role and potential connections to proteins like p53 which is frequently mutated in cancers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-LRWD1 antibody (ab251732)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for LRWD1 (green) using ab251732 at 4 µg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRWD1 antibody (ab251732)
Paraffin-embedded human testis tissue stained for LRWD1 using ab251732 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRWD1 antibody (ab251732)
Paraffin-embedded human prostate tissue stained for LRWD1 using ab251732 at 1/50 dilution in immunohistochemical analysis. Low expression, as expected.
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