JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB251732

Anti-LRWD1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal LRWD1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LRWD1 aa 100-200.

View Alternative Names

CENP-33, ORCA, LRWD1, Leucine-rich repeat and WD repeat-containing protein 1, Centromere protein 33, Origin recognition complex-associated protein, ORC-associated protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-LRWD1 antibody (AB251732)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LRWD1 antibody (AB251732)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for LRWD1 (green) using ab251732 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRWD1 antibody (AB251732)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRWD1 antibody (AB251732)

Paraffin-embedded human prostate tissue stained for LRWD1 using ab251732 at 1/50 dilution in immunohistochemical analysis. Low expression, as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRWD1 antibody (AB251732)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRWD1 antibody (AB251732)

Paraffin-embedded human testis tissue stained for LRWD1 using ab251732 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LRWD1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q9UFC0

style
left-column

Complementary Products

Primary Antibodies

AB188423

Anti-Cdc6 antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody - N-terminal (AB188423)

  • 5
  • 2
Primary Antibodies

AB13579

Anti-TRF2 antibody [4A794]

Flow Cytometry (Intracellular) - Anti-TRF2 antibody [4A794] (AB13579)

  • 5
  • 1
Primary Antibodies

AB113295

Anti-GANP antibody

Western blot - Anti-GANP antibody (AB113295)

  • 0
  • 0
Primary Antibodies

AB10579

Anti-TRF2 + TRF1 antibody [TRF-78]

Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (AB10579)

  • 5
  • 10
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LRWD1 also known as Leucine-rich repeats and WD repeat-containing protein 1 plays an essential mechanical role in cells. This protein weighs approximately 63 kDa and is expressed mainly in tissues such as the testis. LRWD1 has structural domains that include leucine-rich repeats and WD repeat domains contributing to its role in protein interactions and signaling pathways.
Biological function summary

LRWD1 supports the regulation of DNA replication and centrosome cycle. It is an integral part of the DNA replication licensing pathway ensuring proper DNA synthesis during the cell cycle. By participating in this complex LRWD1 collaborates with proteins like CDC6 and CDT1 to initiate DNA replication. This protein ensures that cells duplicate their DNA once per cycle maintaining genomic stability.

Pathways

LRWD1 participates in the DNA replication pathway and is also linked to the G2 to M phase transition of the cell cycle. Through the DNA replication pathway LRWD1 works hand-in-hand with other factors such as ORC1 and MCM proteins which coordinate the licensing of replication origins. Its presence in these pathways demonstrates its pivotal role in maintaining normal cell cycle progression and genomic integrity.

LRWD1 has associations with disorders involving improper DNA replication and genome instability. Misregulation of this protein can relate to conditions such as cancer due to its involvement in cell cycle control. In cancer LRWD1's interaction with DNA replication-related proteins highlights the pathway's relevance in tumorigenesis. Researchers continue to investigate its role and potential connections to proteins like p53 which is frequently mutated in cancers.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability.
See full target information LRWD1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40869249

2025

Methodological Development of a Test for Salivary Proteome Analysis Useful in Lung Cancer Screening.

Applications

Unspecified application

Species

Unspecified reactive species

Leonarda Barra,Elena Carestia,Giulia Ferri,Mohammad Kazemi,Massoumeh Ramahi,Uditanshu Priyadarshi,Velia Di Resta,Fabrizio Di Giuseppe,Renata Ciccarelli,Achille Lococo,Stefania Angelucci
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com