AB234863

Anti-LSD2 / AOF1 antibody

Name: Anti-LSD2 / AOF1 antibody (ab234863) | Abcam
Brand: Abcam Limited
SKU: AB234863
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal LSD2 / AOF1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KDM1B aa 450-550.

View Alternative Names

AOF1, C6orf193, LSD2, KDM1B, Lysine-specific histone demethylase 2, Flavin-containing amine oxidase domain-containing protein 1, Lysine-specific histone demethylase 1B

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSD2 / AOF1 antibody (AB234863)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSD2 / AOF1 antibody (AB234863)

Paraffin-embedded human liver tissue stained for LSD2 / AOF1 using ab234863 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSD2 / AOF1 antibody (AB234863)

Paraffin-embedded human kidney tissue stained for LSD2 / AOF1 using ab234863 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KDM1B aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q8NB78

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSD2 also known as AOF1 is a lysine-specific demethylase enzyme with a mass of about 105 kDa. This protein mainly functions by removing methyl groups from lysine residues on histone proteins thereby affecting chromatin structure and gene expression. LSD2 is expressed in various tissues with high levels in the liver and brain. The enzyme plays a significant role in regulating gene activity influencing many biological processes.

Biological function summary

LSD2 impacts gene expression by altering the epigenetic state of chromatin. It is not part of a well-defined large multiprotein complex allowing LSD2 to directly interact with histones. Through its demethylase activity it modulates the transcriptional regulation impacting cellular differentiation and proliferation. This makes it an important factor in maintaining cellular homeostasis.

Pathways

LSD2 is involved in histone modification processes particularly through the demethylation of H3K4me1 and H3K4me2 marks. These changes are important in the regulation of gene expression patterns. LSD2 often interacts with the REST corepressor complex affecting the expression of genes involved in neural development and function. It plays a role in pathways related to cellular stress responses and metabolic regulation highlighting its importance in cellular adaptation mechanisms.

LSD2 has been implicated in cancer and neurological disorders. Alterations in its expression and activity are associated with certain types of cancers such as breast cancer where it affects tumor growth and progression by influencing gene expression patterns. In neurological contexts LSD2's dysregulation impacts neural circuitry and cognitive functions which can be linked to disorders like Alzheimer's disease. In these conditions LSD2 interacts with proteins such as SIRT1 influencing cellular processes that contribute to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of GLYR1 to achieve such activity, they form a multifunctional enzyme complex that modifies transcribed chromatin and facilitates Pol II transcription through nucleosomes (PubMed : 30970244).

See full target information KDM1B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 8:eadd3339 PubMed36197977

2022

CARM1-mediated methylation of ASXL2 impairs tumor-suppressive function of MLL3/COMPASS.

Applications

Unspecified application

Species

Unspecified reactive species

Zibo Zhao,Emily Jane Rendleman,Aileen Patricia Szczepanski,Marc Alard Morgan,Lu Wang,Ali Shilatifard

Cell death discovery 8:274 PubMed35668081

2022

SMYD2 aggravates gastrointestinal stromal tumor via upregulation of EZH2 and downregulation of TET1.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Ji,Xiaofeng Xu,Cong Long,Jianjiang Wang,Li Ding,Zhizhong Zheng,Huiping Wu,Liu Yang,Lan Tao,Feng Gao

Aging 12:14990-15001 PubMed32726297

2020

Lysine-specific histone demethylase 1B (LSD2/KDM1B) represses p53 expression to promote proliferation and inhibit apoptosis in colorectal cancer through LSD2-mediated H3K4me2 demethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoxin Cai,Jinsi Wang,Wei Zeng,Xuefei Cheng,Lihang Liu,Weihua Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LSD2 / AOF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSD2 / AOF1 antibody (AB234863)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSD2 / AOF1 antibody (AB234863)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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