AB181196

Anti-LSECtin antibody [EPR13724]

RabMAb
Recombinant
What is this?

(1 Review)

(6 Publications)

Anti-LSECtin antibody [EPR13724] (ab181196) is a rabbit monoclonal antibody detecting LSECtin in Western Blot, IHC-P. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

UNQ431/PRO792, CLEC4G, C-type lectin domain family 4 member G, Liver and lymph node sinusoidal endothelial cell C-type lectin, LSECtin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (AB181196)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (AB181196)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody : anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (AB181196)

Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody : anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-LSECtin antibody [EPR13724] (AB181196)

All lanes:

Western blot - Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human lymph node lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 33 kDa

false

Lab

Western blot - Anti-LSECtin antibody [EPR13724] (AB181196)

All lanes:

Western blot - Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 40 kDa

false

Exposure time: 180s

Carrier free

Anti-LSECtin antibody [EPR13724] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13724

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-LSECtin antibody [EPR13724] (ab181196) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of LSECtin?
Anti-LSECtin [EPR13724] (ab181196) specifically detects a band for LSECtin (UniProt: Q6UXB4) at a molecular weight of 33kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR13724] also available for your convenience: ab181196, Carrier free - ab250419

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSECtin also known as CLEC4G is a C-type lectin-like receptor weighing around 40 kDa. It is expressed mainly on liver sinusoidal endothelial cells (LSECs) but also noted in lymph node and placenta tissues. As a receptor LSECtin plays an important role in recognizing and binding to specific glycans on pathogens facilitating their capture and subsequent immune response.

Biological function summary

LSECtin functions as an important player in immune recognition and clearance of pathogens. It is involved in pathogen binding through interaction with glycans aiding in the modulation of immune responses. LSECtin along with other related receptors like DC-SIGN and CD23 forms part of a complex that influences pathogen recognition. It particularly interacts with viruses like HIV and Ebola impacting how these pathogens are detected and processed by the immune system.

Pathways

The function of LSECtin integrates into immune system pathways where it influences pathogen clearance. One notable pathway is the pathogen recognition pathway. LSECtin interacts with the liver's immune regulatory network linking with proteins like DC-SIGN that play a role in modulating the immune response. Another pathway it affects is the cell adhesion pathway which involves inflammation regulation.

LSECtin is linked to infections and immune-related liver conditions. In viral infections such as Ebola and HIV LSECtin mediates pathogen capture affecting the body's ability to handle these infections. The receptor's interaction with liver-specific proteins is noted in liver fibrosis where LSECtin and related proteins like CD23 may alter immune function and disease progression. This close relationship highlights the importance of LSECtin in both immune defense and disease pathology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Binds mannose, N-acetylglucosamine (GlcNAc) and fucose, but not galactose, in a Ca(2+)-dependent manner, in vitro.. (Microbial infection) Acts as a receptor for Japanese encephalitis virus.. (Microbial infection) Acts as a receptor for Ebolavirus.. (Microbial infection) Acts as a receptor for SARS-CoV.. (Microbial infection) Acts as a receptor for Lassa virus and Lymphocytic choriomeningitis virus glycoprotein (PubMed : 22156524, PubMed : 22673088).

See full target information CLEC4G

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 15:1452739 PubMed39749135

2025

Limitations of a proper SFTSV mouse model using human C-type lectin receptors.

Applications

Unspecified application

Species

Unspecified reactive species

You-Min Kim,Hyo-Jin Ro,Jae Hoon Lee,Yaechan Song,Han-Woong Lee,Nam-Hyuk Cho

Frontiers in immunology 15:1302761 PubMed38390332

2024

Immune checkpoint ligands expressed on mature high endothelial venules predict poor prognosis of NSCLC: have a relationship with CD8 T lymphocytes infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Luo,Xiuhuan Shi,Yumeng Liu,Jian Wang,Hao Wang,Xuena Yang,Qian Sun,Zhenzhen Hui,Feng Wei,Xiubao Ren,Hua Zhao

Molecular therapy : the journal of the American Society of Gene Therapy 32:952-968 PubMed38327046

2024

High-dose systemic adeno-associated virus vector administration causes liver and sinusoidal endothelial cell injury.

Applications

Unspecified application

Species

Unspecified reactive species

Juliette Hordeaux,R Jason Lamontagne,Chunjuan Song,George Buchlis,Cecilia Dyer,Elizabeth L Buza,Ali Ramezani,Erik Wielechowski,Jenny A Greig,Jessica A Chichester,Peter Bell,James M Wilson

Cancers 14: PubMed36358719

2022

CXCL8 Up-Regulated LSECtin through AKT Signal and Correlates with the Immune Microenvironment Modulation in Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojun Fang,Xianshuo Cheng,Tao Shen,Jian Dong,Yunfeng Li,Zhenhui Li,Linghan Tian,Yangwei Zhang,Xueyan Pan,Zhengfeng Yin,Zhibin Yang

Nature communications 11:6352 PubMed33311518

2020

An immune checkpoint score system for prognostic evaluation and adjuvant chemotherapy selection in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Bin Wang,Ping Li,Xiao-Long Liu,Qiao-Ling Zheng,Yu-Bin Ma,Ya-Jun Zhao,Jian-Wei Xie,Jian-Xian Lin,Jun Lu,Qi-Yue Chen,Long-Long Cao,Mi Lin,Li-Chao Liu,Ning-Zi Lian,Ying-Hong Yang,Chang-Ming Huang,Chao-Hui Zheng

The FEBS journal 286:4937-4950 PubMed31408583

2019

MROH7-TTC4 read-through lncRNA suppresses vascular endothelial cell apoptosis and is upregulated by inhibition of ANXA7 GTPase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying He,Xuan Zhao,Le Su,Baoxiang Zhao,Junying Miao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LSECtin antibody [EPR13724]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (AB181196)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] (AB181196)

Western blot - Anti-LSECtin antibody [EPR13724] (AB181196)

Western blot - Anti-LSECtin antibody [EPR13724] (AB181196)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise