Skip to main content

Rabbit Recombinant Monoclonal LSECtin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419), expandable thumbnail
  • Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419), expandable thumbnail
  • Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-P
Human
Not recommended
Tested
Tested
Mouse
Not recommended
Tested
Expected

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Binds mannose, N-acetylglucosamine (GlcNAc) and fucose, but not galactose, in a Ca(2+)-dependent manner, in vitro.(Microbial infection) Acts as a receptor for Japanese encephalitis virus.(Microbial infection) Acts as a receptor for Ebolavirus.(Microbial infection) Acts as a receptor for SARS-CoV.(Microbial infection) Acts as a receptor for Lassa virus and Lymphocytic choriomeningitis virus glycoprotein (PubMed:22156524, PubMed:22673088).

Alternative names

UNQ431/PRO792, CLEC4G, UNQ431/PRO792, C-type lectin domain family 4 member G, Liver and lymph node sinusoidal endothelial cell C-type lectin, LSECtin

Recommended products

Rabbit Recombinant Monoclonal LSECtin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR13724

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab250419 is the carrier-free version of Anti-LSECtin antibody [EPR13724] ab181196.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

LSECtin also known as CLEC4G is a C-type lectin-like receptor weighing around 40 kDa. It is expressed mainly on liver sinusoidal endothelial cells (LSECs) but also noted in lymph node and placenta tissues. As a receptor LSECtin plays an important role in recognizing and binding to specific glycans on pathogens facilitating their capture and subsequent immune response.

Biological function summary

LSECtin functions as an important player in immune recognition and clearance of pathogens. It is involved in pathogen binding through interaction with glycans aiding in the modulation of immune responses. LSECtin along with other related receptors like DC-SIGN and CD23 forms part of a complex that influences pathogen recognition. It particularly interacts with viruses like HIV and Ebola impacting how these pathogens are detected and processed by the immune system.

Pathways

The function of LSECtin integrates into immune system pathways where it influences pathogen clearance. One notable pathway is the pathogen recognition pathway. LSECtin interacts with the liver's immune regulatory network linking with proteins like DC-SIGN that play a role in modulating the immune response. Another pathway it affects is the cell adhesion pathway which involves inflammation regulation.

Associated diseases and disorders

LSECtin is linked to infections and immune-related liver conditions. In viral infections such as Ebola and HIV LSECtin mediates pathogen capture affecting the body's ability to handle these infections. The receptor's interaction with liver-specific proteins is noted in liver fibrosis where LSECtin and related proteins like CD23 may alter immune function and disease progression. This close relationship highlights the importance of LSECtin in both immune defense and disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)

    This data was developed using Anti-LSECtin antibody [EPR13724] ab181196, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling LSECtin with Anti-LSECtin antibody [EPR13724] ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419), expandable thumbnail

    Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)

    This data was developed using Anti-LSECtin antibody [EPR13724] ab181196, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-LSECtin antibody [EPR13724] (Anti-LSECtin antibody [EPR13724] ab181196) at 1/1000 dilution

    All lanes: Mouse liver tissue lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 40 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)

    This data was developed using Anti-LSECtin antibody [EPR13724] ab181196, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LSECtin with Anti-LSECtin antibody [EPR13724] ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419), expandable thumbnail

    Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (ab250419)

    This data was developed using Anti-LSECtin antibody [EPR13724] ab181196, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-LSECtin antibody [EPR13724] (Anti-LSECtin antibody [EPR13724] ab181196) at 1/1000 dilution

    Lane 1: Human fetal liver lysate at 20 µg

    Lane 2: Human lymph node lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution

    Predicted band size: 33 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com