AB250419

Anti-LSECtin antibody [EPR13724] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal LSECtin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

UNQ431/PRO792, CLEC4G, C-type lectin domain family 4 member G, Liver and lymph node sinusoidal endothelial cell C-type lectin, LSECtin

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419)

This data was developed using ab181196, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody : anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419)

This data was developed using ab181196, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody : anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419)

This data was developed using ab181196, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LSECtin antibody [EPR13724] (<a href='/en-us/products/primary-antibodies/lsectin-antibody-epr13724-ab181196'>ab181196</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human lymph node lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 33 kDa

false

Lab

Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419)

This data was developed using ab181196, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LSECtin antibody [EPR13724] (<a href='/en-us/products/primary-antibodies/lsectin-antibody-epr13724-ab181196'>ab181196</a>) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 33 kDa

Observed band size: 40 kDa

false

Exposure time: 180s

Unconjugated

Anti-LSECtin antibody [EPR13724]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13724

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250419 is the carrier-free version of ab181196.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSECtin also known as CLEC4G is a C-type lectin-like receptor weighing around 40 kDa. It is expressed mainly on liver sinusoidal endothelial cells (LSECs) but also noted in lymph node and placenta tissues. As a receptor LSECtin plays an important role in recognizing and binding to specific glycans on pathogens facilitating their capture and subsequent immune response.

Biological function summary

LSECtin functions as an important player in immune recognition and clearance of pathogens. It is involved in pathogen binding through interaction with glycans aiding in the modulation of immune responses. LSECtin along with other related receptors like DC-SIGN and CD23 forms part of a complex that influences pathogen recognition. It particularly interacts with viruses like HIV and Ebola impacting how these pathogens are detected and processed by the immune system.

Pathways

The function of LSECtin integrates into immune system pathways where it influences pathogen clearance. One notable pathway is the pathogen recognition pathway. LSECtin interacts with the liver's immune regulatory network linking with proteins like DC-SIGN that play a role in modulating the immune response. Another pathway it affects is the cell adhesion pathway which involves inflammation regulation.

LSECtin is linked to infections and immune-related liver conditions. In viral infections such as Ebola and HIV LSECtin mediates pathogen capture affecting the body's ability to handle these infections. The receptor's interaction with liver-specific proteins is noted in liver fibrosis where LSECtin and related proteins like CD23 may alter immune function and disease progression. This close relationship highlights the importance of LSECtin in both immune defense and disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds mannose, N-acetylglucosamine (GlcNAc) and fucose, but not galactose, in a Ca(2+)-dependent manner, in vitro.. (Microbial infection) Acts as a receptor for Japanese encephalitis virus.. (Microbial infection) Acts as a receptor for Ebolavirus.. (Microbial infection) Acts as a receptor for SARS-CoV.. (Microbial infection) Acts as a receptor for Lassa virus and Lymphocytic choriomeningitis virus glycoprotein (PubMed : 22156524, PubMed : 22673088).

See full target information CLEC4G

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LSECtin antibody [EPR13724] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419)

Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419)

Western blot - Anti-LSECtin antibody [EPR13724] - BSA and Azide free (AB250419)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise