AB180128

Anti-LSM10 antibody [EPR13843]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal LSM10 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

U7 snRNA-associated Sm-like protein LSm10, LSM10

2 Images

Supplier Data

Western blot - Anti-LSM10 antibody [EPR13843] (AB180128)

All lanes:

Western blot - Anti-LSM10 antibody [EPR13843] (ab180128) at 1/5000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

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Supplier Data

Immunoprecipitation - Anti-LSM10 antibody [EPR13843] (AB180128)

Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab180128 at 1/40 dilution. Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-LSM10 antibody [EPR13843] (ab180128)

Predicted band size: 14 kDa

false

Carrier free

Anti-LSM10 antibody [EPR13843] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13843

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSM10 also known as Sm-like protein LSm10 is a component of the LSm protein family with an approximate molecular mass of 10 kDa. This protein is ubiquitously expressed in various tissues playing a role predominantly in the nucleus. LSM10 is involved in the assembly and functioning of ribonucleoproteins related to its role in RNA processing and regulation.

Biological function summary

The role of LSM10 engages in RNA splicing and modification processes as part of a small nuclear ribonucleoprotein (snRNP) complex. Within the complex LSM10 contributes to the stability and assembly of snRNP particles facilitating the proper maturation of RNA molecules. LSM10's presence is critical for the formation of U7 snRNP which specifically addresses histone pre-mRNA processing and ensures efficient recycling and turnover of RNA.

Pathways

The protein functions prominently in RNA splicing pathways and histone RNA processing. LSM10 interacts with factors like LSM11 and other related proteins that assist in stabilizing RNA structures necessary for accurate gene regulation. The protein plays an integral role in connective pathways that facilitate histone mRNA transcription regulation and turnover involving partners such as the U7 snRNP complex and other splicing factors.

LSM10 exhibits links to conditions like spinal muscular atrophy and certain cancers. Alterations in the LSM10-related pathway might impact snRNP assembly influencing the molecular mechanisms behind these diseases. The protein connects to SMN1 known for its involvement in snRNP complex stability a factor critical in spinal muscular atrophy. Moreover aberrations in LSM10 activity or expression might contribute to cancer pathogenesis through disrupted RNA processing and gene expression regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Increases U7 snRNA levels but not histone 3'-end pre-mRNA processing activity, when overexpressed. Required for cell cycle progression from G1 to S phases. Binds specifically to U7 snRNA. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.

See full target information LSM10

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American Society of Gene Therapy 29:2167-2181 PubMed33578037

2021

RETRACTED: circTulp4 functions in Alzheimer’s disease pathogenesis by regulating its parental gene, Tulp4

Applications

Unspecified application

Species

Unspecified reactive species

Nana Ma,Jie Pan,Yi Wen,Qi Wu,Bo Yu,Xi Chen,Jun Wan,Wei Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LSM10 antibody [EPR13843]

Western blot - Anti-LSM10 antibody [EPR13843] (AB180128)

Immunoprecipitation - Anti-LSM10 antibody [EPR13843] (AB180128)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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