JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250140

Anti-LSM10 antibody [EPR13843] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal LSM10 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

U7 snRNA-associated Sm-like protein LSm10, LSM10

2 Images
Immunoprecipitation - Anti-LSM10 antibody [EPR13843] - BSA and Azide free (AB250140)
  • IP

Supplier Data

Immunoprecipitation - Anti-LSM10 antibody [EPR13843] - BSA and Azide free (AB250140)

This data was developed using ab180128, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab180128 at 1/40 dilution. Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-LSM10 antibody [EPR13843] (<a href='/en-us/products/primary-antibodies/lsm10-antibody-epr13843-ab180128'>ab180128</a>)

false

Western blot - Anti-LSM10 antibody [EPR13843] - BSA and Azide free (AB250140)
  • WB

Supplier Data

Western blot - Anti-LSM10 antibody [EPR13843] - BSA and Azide free (AB250140)

This data was developed using ab180128, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LSM10 antibody [EPR13843] (<a href='/en-us/products/primary-antibodies/lsm10-antibody-epr13843-ab180128'>ab180128</a>) at 1/5000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13843

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Product details

ab250140 is the carrier-free version of ab180128.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSM10 also known as Sm-like protein LSm10 is a component of the LSm protein family with an approximate molecular mass of 10 kDa. This protein is ubiquitously expressed in various tissues playing a role predominantly in the nucleus. LSM10 is involved in the assembly and functioning of ribonucleoproteins related to its role in RNA processing and regulation.
Biological function summary

The role of LSM10 engages in RNA splicing and modification processes as part of a small nuclear ribonucleoprotein (snRNP) complex. Within the complex LSM10 contributes to the stability and assembly of snRNP particles facilitating the proper maturation of RNA molecules. LSM10's presence is critical for the formation of U7 snRNP which specifically addresses histone pre-mRNA processing and ensures efficient recycling and turnover of RNA.

Pathways

The protein functions prominently in RNA splicing pathways and histone RNA processing. LSM10 interacts with factors like LSM11 and other related proteins that assist in stabilizing RNA structures necessary for accurate gene regulation. The protein plays an integral role in connective pathways that facilitate histone mRNA transcription regulation and turnover involving partners such as the U7 snRNP complex and other splicing factors.

LSM10 exhibits links to conditions like spinal muscular atrophy and certain cancers. Alterations in the LSM10-related pathway might impact snRNP assembly influencing the molecular mechanisms behind these diseases. The protein connects to SMN1 known for its involvement in snRNP complex stability a factor critical in spinal muscular atrophy. Moreover aberrations in LSM10 activity or expression might contribute to cancer pathogenesis through disrupted RNA processing and gene expression regulation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Increases U7 snRNA levels but not histone 3'-end pre-mRNA processing activity, when overexpressed. Required for cell cycle progression from G1 to S phases. Binds specifically to U7 snRNA. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
See full target information LSM10
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com