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AB249753

Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free

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Rabbit Recombinant Monoclonal LSM12 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Protein LSM12, LSM12

4 Images
Western blot - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (AB249753)
  • WB

Supplier Data

Western blot - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (AB249753)

This data was developed using ab173292, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LSM12 antibody [EPR12282-87] (<a href='/en-us/products/primary-antibodies/lsm12-antibody-epr12282-87-ab173292'>ab173292</a>) at 1/10000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Fetal brain tissue lysate at 10 µg

Predicted band size: 22 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (AB249753)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (AB249753)

This data was developed using ab173292, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling LSM12 with ab173292 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (AB249753)
  • IP

Supplier Data

Immunoprecipitation - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (AB249753)

This data was developed using ab173292, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from 1) HeLa cell lysate or 2) 1X PBS using ab173292 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-LSM12 antibody [EPR12282-87] (<a href='/en-us/products/primary-antibodies/lsm12-antibody-epr12282-87-ab173292'>ab173292</a>)

Predicted band size: 22 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (AB249753)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282-87] - BSA and Azide free (AB249753)

This data was developed using ab173292, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173292 at 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12282-87

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab249753 is the carrier-free version of ab173292.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSM12 also known as LSM12 homolog is a protein with a molecular weight of approximately 16 kDa. This protein is part of the Sm-like family known for their role in RNA processing. LSM12 is ubiquitously expressed in various tissues but shows higher presence in brain tissue indicating its potential importance in neurological functions. Its expression patterns suggest that LSM12 may have diverse roles across different cellular contexts.
Biological function summary

This protein is involved in cellular processes associated with mRNA decay and stability. LSM12 is a component of the LSM protein complex which plays an important role in RNA metabolism. The complex ensures accurate degradation and processing of mRNAn important steps required for maintaining cellular RNA homeostasis. Within the complex LSM12 interacts with other LSM proteins to promote efficient RNA handling and control.

Pathways

LSM12 is associated with RNA processing pathways including the nonsense-mediated decay (NMD) pathway. This pathway ensures the elimination of faulty mRNA transcripts which if left unchecked can disrupt cellular functions. In these pathways LSM12 may interact with UPF1 a well-known protein involved in NMD aiding in identifying and degrading erroneous mRNA molecules. This suggests that LSM12 supports the maintenance of genomic integrity through RNA surveillance mechanisms.

Researchers link LSM12 to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. These connections highlight the significance of proper RNA metabolism in neuronal function. LSM12's interaction with other RNA processing proteins such as TDP-43 in ALS suggests a potential disruption of RNA stability as a contributing factor in these diseases. The study of LSM12 offers valuable insights into therapeutic targets for neurological conditions related to mRNA dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein (PubMed : 34362892). Confers NAADP sensitivity to the two pore channel complex (TPCs) by acting as TPC accessory protein necessary for NAADP-evoked Ca(2+) release (PubMed : 34362892).
See full target information LSM12
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com