AB173291

Anti-LSM12 antibody [EPR12282]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Recombinant Monoclonal LSM12 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Protein LSM12, LSM12

4 Images

Flow Cytometry (Intracellular) - Anti-LSM12 antibody [EPR12282] (AB173291)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LSM12 antibody [EPR12282] (AB173291)

Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling LSM12 with ab173291 at 1/10 dilution (red),compared to arabbit IgG negative control (green).

Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282] (AB173291)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282] (AB173291)

Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173291 at 1/100 dilution.

Unknown

Immunoprecipitation - Anti-LSM12 antibody [EPR12282] (AB173291)

Western blot analysis on immunoprecipitation pellet from 1) HepG2 cell lysate or 2) 1X PBS (negative control) using ab173291 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-LSM12 antibody [EPR12282] (ab173291)

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-LSM12 antibody [EPR12282] (AB173291)

All lanes:

Western blot - Anti-LSM12 antibody [EPR12282] (ab173291) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 22 kDa

false

Carrier free

Anti-LSM12 antibody [EPR12282] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12282

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSM12 also known as LSM12 homolog is a protein with a molecular weight of approximately 16 kDa. This protein is part of the Sm-like family known for their role in RNA processing. LSM12 is ubiquitously expressed in various tissues but shows higher presence in brain tissue indicating its potential importance in neurological functions. Its expression patterns suggest that LSM12 may have diverse roles across different cellular contexts.

Biological function summary

This protein is involved in cellular processes associated with mRNA decay and stability. LSM12 is a component of the LSM protein complex which plays an important role in RNA metabolism. The complex ensures accurate degradation and processing of mRNAn important steps required for maintaining cellular RNA homeostasis. Within the complex LSM12 interacts with other LSM proteins to promote efficient RNA handling and control.

Pathways

LSM12 is associated with RNA processing pathways including the nonsense-mediated decay (NMD) pathway. This pathway ensures the elimination of faulty mRNA transcripts which if left unchecked can disrupt cellular functions. In these pathways LSM12 may interact with UPF1 a well-known protein involved in NMD aiding in identifying and degrading erroneous mRNA molecules. This suggests that LSM12 supports the maintenance of genomic integrity through RNA surveillance mechanisms.

Researchers link LSM12 to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. These connections highlight the significance of proper RNA metabolism in neuronal function. LSM12's interaction with other RNA processing proteins such as TDP-43 in ALS suggests a potential disruption of RNA stability as a contributing factor in these diseases. The study of LSM12 offers valuable insights into therapeutic targets for neurological conditions related to mRNA dysfunction.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein (PubMed : 34362892). Confers NAADP sensitivity to the two pore channel complex (TPCs) by acting as TPC accessory protein necessary for NAADP-evoked Ca(2+) release (PubMed : 34362892).

See full target information LSM12

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012535 PubMed39255317

2024

Methylation of KSHV vCyclin by PRMT5 contributes to cell cycle progression and cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Danping Niu,Yuanming Ma,Pengyu Ren,Sijia Chang,Chenhui Li,Yong Jiang,Chunyan Han,Ke Lan

Science signaling 16:eadg0661 PubMed37607219

2023

Convergent activation of Ca permeability in two-pore channel 2 through distinct molecular routes.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Saito,Qianru Mu,Yu Yuan,Marcos Rubio-Alarcón,Maria Eznarriaga,Pingwei Zhao,Gihan Gunaratne,Sushil Kumar,Marco Keller,Franz Bracher,Christian Grimm,Eugen Brailoiu,Jonathan S Marchant,Taufiq Rahman,Sandip Patel

Science signaling 16:eadg0485 PubMed37607218

2023

Convergent activation of two-pore channels mediated by the NAADP-binding proteins JPT2 and LSM12.

Applications

Unspecified application

Species

Unspecified reactive species

Gihan S Gunaratne,Eugen Brailoiu,Sushil Kumar,Yu Yuan,James T Slama,Timothy F Walseth,Sandip Patel,Jonathan S Marchant

Oncology research 30:289-300 PubMed37303493

2023

LSM12 facilitates the progression of colorectal cancer by activating the WNT/CTNNB1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhuang,Chunlan Ning,Pengfei Liu,Yanpeng Zhao,Yue Li,Zhenchi Ma,Luling Shan,Yingzhe Piao,Peng Zhao,Xun Jin

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LSM12 antibody [EPR12282]

Flow Cytometry (Intracellular) - Anti-LSM12 antibody [EPR12282] (AB173291)

Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282] (AB173291)

Immunoprecipitation - Anti-LSM12 antibody [EPR12282] (AB173291)

Western blot - Anti-LSM12 antibody [EPR12282] (AB173291)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise