JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB173291

Anti-LSM12 antibody [EPR12282]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal LSM12 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Protein LSM12, LSM12

4 Images
Western blot - Anti-LSM12 antibody [EPR12282] (AB173291)
  • WB

Supplier Data

Western blot - Anti-LSM12 antibody [EPR12282] (AB173291)

All lanes:

Western blot - Anti-LSM12 antibody [EPR12282] (ab173291) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 22 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282] (AB173291)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282] (AB173291)

Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173291 at 1/100 dilution.

Immunoprecipitation - Anti-LSM12 antibody [EPR12282] (AB173291)
  • IP

Unknown

Immunoprecipitation - Anti-LSM12 antibody [EPR12282] (AB173291)

Western blot analysis on immunoprecipitation pellet from 1) HepG2 cell lysate or 2) 1X PBS (negative control) using ab173291 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-LSM12 antibody [EPR12282] (ab173291)

Predicted band size: 22 kDa

false

Flow Cytometry (Intracellular) - Anti-LSM12 antibody [EPR12282] (AB173291)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LSM12 antibody [EPR12282] (AB173291)

Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling LSM12 with ab173291 at 1/10 dilution (red),compared to arabbit IgG negative control (green).

  • Carrier free

    Anti-LSM12 antibody [EPR12282] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12282

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSM12 also known as LSM12 homolog is a protein with a molecular weight of approximately 16 kDa. This protein is part of the Sm-like family known for their role in RNA processing. LSM12 is ubiquitously expressed in various tissues but shows higher presence in brain tissue indicating its potential importance in neurological functions. Its expression patterns suggest that LSM12 may have diverse roles across different cellular contexts.
Biological function summary

This protein is involved in cellular processes associated with mRNA decay and stability. LSM12 is a component of the LSM protein complex which plays an important role in RNA metabolism. The complex ensures accurate degradation and processing of mRNAn important steps required for maintaining cellular RNA homeostasis. Within the complex LSM12 interacts with other LSM proteins to promote efficient RNA handling and control.

Pathways

LSM12 is associated with RNA processing pathways including the nonsense-mediated decay (NMD) pathway. This pathway ensures the elimination of faulty mRNA transcripts which if left unchecked can disrupt cellular functions. In these pathways LSM12 may interact with UPF1 a well-known protein involved in NMD aiding in identifying and degrading erroneous mRNA molecules. This suggests that LSM12 supports the maintenance of genomic integrity through RNA surveillance mechanisms.

Researchers link LSM12 to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. These connections highlight the significance of proper RNA metabolism in neuronal function. LSM12's interaction with other RNA processing proteins such as TDP-43 in ALS suggests a potential disruption of RNA stability as a contributing factor in these diseases. The study of LSM12 offers valuable insights into therapeutic targets for neurological conditions related to mRNA dysfunction.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Nicotinic acid adenine dinucleotide phosphate (NAADP) binding protein (PubMed : 34362892). Confers NAADP sensitivity to the two pore channel complex (TPCs) by acting as TPC accessory protein necessary for NAADP-evoked Ca(2+) release (PubMed : 34362892).
See full target information LSM12
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB173292

Anti-LSM12 antibody [EPR12282-87]

primary-antibodies

lsm12-antibody-epr12282-87-ab173292

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com