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AB236744

Anti-LSM4 antibody

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Rabbit Polyclonal LSM4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LSM4.

View Alternative Names

U6 snRNA-associated Sm-like protein LSm4, Glycine-rich protein, GRP, LSM4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-LSM4 antibody (AB236744)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LSM4 antibody (AB236744)

MCF7 (Human breast adenocarcinoma cell line) cells stained for LSM4 (green) using ab236744 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM4 antibody (AB236744)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM4 antibody (AB236744)

Paraffin-embedded human adrenal gland tissue stained for LSM4 using ab236744 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-LSM4 antibody (AB236744)
  • WB

Supplier Data

Western blot - Anti-LSM4 antibody (AB236744)

All lanes:

Western blot - Anti-LSM4 antibody (ab236744) at 1/500 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LSM4.

Q9Y4Z0

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSM4 also known as Like-Sm protein 4 is a component of the LSM protein family with a molecular mass of approximately 12 kDa. It plays a mechanical role in the formation of the LSM complex which is similar to the Sm proteins involved in RNA processing. Expression of LSM4 occurs in various tissues including the brain and immune cells indicating its diverse functional significance across different biological systems.
Biological function summary

Like-Sm protein 4 plays an important role in RNA processing particularly in mRNA splicing and degradation. It forms part of the LSm1-7 complex which associates with the U6 snRNA to regulate the splicing mechanisms necessary for proper gene expression. By ensuring accurate mRNA splicing LSM4 contributes to the maintenance of cellular function and homeostasis.

Pathways

LSM4's involvement in RNA splicing positions it within the spliceosome pathway essential for processing pre-mRNA into mature mRNA. Through this pathway it collaborates with other LSm proteins and the DEAD-box helicase DDX41. LSM4 also has a role in the RNA decay pathway where it interacts with the XRN1 protein facilitating the turnover of mRNA to control gene expression levels in cells.

Errors in LSM4 function may lead to neurological disorders such as spinal muscular atrophy. This association occurs through its interaction with the SMN protein where disruptions in RNA splicing affect motor neuron survival. LSM4 also associates with cancerous conditions as improper mRNA processing can lead to oncogene activation influencing processes like cell proliferation and apoptosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed : 28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed : 10523320).
See full target information LSM4
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Product promise

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