Rabbit Recombinant Monoclonal LSM5 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (AB250769), expandable thumbnail](https://content.abcam.com/products/images/lsm5-antibody-epr10690-bsa-and-azide-free-ab250769--flow-cytometry-intracellular-img63636.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (AB250769)")
![Immunocytochemistry/ Immunofluorescence - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (AB250769), expandable thumbnail](https://content.abcam.com/products/images/lsm5-antibody-epr10690-bsa-and-azide-free-ab250769--immunocytochemistry-immunofluorescence-img13835.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (AB250769)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (AB250769), expandable thumbnail](https://content.abcam.com/products/images/lsm5-antibody-epr10690-bsa-and-azide-free-ab250769--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7546.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (AB250769)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (AB250769), expandable thumbnail](https://content.abcam.com/products/images/lsm5-antibody-epr10690-bsa-and-azide-free-ab250769--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115024.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (AB250769)")
![Western blot - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (AB250769), expandable thumbnail](https://content.abcam.com/products/images/lsm5-antibody-epr10690-bsa-and-azide-free-ab250769--western-blot-img81930.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (AB250769)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Version
Target data
Function
Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed:28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed:10523320).
Alternative names
U6 snRNA-associated Sm-like protein LSm5, LSM5
Recommended products
Rabbit Recombinant Monoclonal LSM5 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10690
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250769 is the carrier-free version of Anti-LSM5 antibody [EPR10690] ab184568.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LSM5 also known as U6 snRNA-associated Sm-like protein LSm5 plays an important role in the mechanics of RNA processing. This protein has a mass of approximately 9.7 kDa. LSM5 is expressed in various tissues with notable concentrations found in the cytoplasm and nucleus. It functions as part of the larger Lsm protein family which is known for playing roles in mRNA degradation and splicing.
- Biological function summary
LSM5 participates in the formation and stabilization of the U6 snRNA. As a member of the Lsm complex it supports RNA processing and stability. The Lsm complex typically consists of seven Lsm proteins including LSM5 forming a heptameric ring structure. This complex assists in the splicing of pre-mRNA a critical step in the regulation of gene expression. By binding to the 3’ end of U6 snRNA LSM5 facilitates the splicing mechanism necessary for proper mRNA maturation.
- Pathways
LSM5 is actively involved in the RNA splicing pathway an important step for gene expression and mRNA maturation. One notable pathway it participates in is the spliceosome cycle closely associated with the processing of pre-mRNA into mature mRNA. In these pathways LSM5 interacts with other components such as U6 snRNP and helps in regulating spliceosomal dynamics. This highlights LSM5's role in maintaining the fidelity and efficiency of mRNA splicing.
- Associated diseases and disorders
LSM5 links to certain conditions stemming from defective RNA processing. One example is spinal muscular atrophy a disorder that affects the motor neurons due to impaired splicing. LSM5 also associates with amyotrophic lateral sclerosis (ALS) which involves RNA metabolism disruption. In these diseases LSM5's interaction with proteins like SMN (survival of motor neuron) and TDP-43 can disrupt normal cellular functions and lead to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry (Intracellular) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769), expandable thumbnail](https://content.abcam.com/products/images/lsm5-antibody-epr10690-bsa-and-azide-free-ab250769--flow-cytometry-intracellular-img63636.jpg "Flow Cytometry (Intracellular) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)")
Flow Cytometry (Intracellular) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
This data was developed using Anti-LSM5 antibody [EPR10690] ab184568, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of T47-D cells using Anti-LSM5 antibody [EPR10690] ab184568 at a 1/110 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769), expandable thumbnail](https://content.abcam.com/products/images/lsm5-antibody-epr10690-bsa-and-azide-free-ab250769--immunocytochemistry-immunofluorescence-img13835.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)")
Immunocytochemistry/ Immunofluorescence - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
This data was developed using Anti-LSM5 antibody [EPR10690] ab184568, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of 4% paraformaldehyde fixed T47-D cells labeling LSM5 with Anti-LSM5 antibody [EPR10690] ab184568 at a 1/500 dilution. Goat anti rabbit IgG (Alexa Fluor®555) secondary used at a 1/200 diution. Conterstained with Dapi (right image).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769), expandable thumbnail](https://content.abcam.com/products/images/lsm5-antibody-epr10690-bsa-and-azide-free-ab250769--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7546.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
This data was developed using Anti-LSM5 antibody [EPR10690] ab184568, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling LSM5 with Anti-LSM5 antibody [EPR10690] ab184568 a 1/100 dilution. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769), expandable thumbnail](https://content.abcam.com/products/images/lsm5-antibody-epr10690-bsa-and-azide-free-ab250769--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115024.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
This data was developed using Anti-LSM5 antibody [EPR10690] ab184568, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human astrocytoma tissue labeling LSM5 with Anti-LSM5 antibody [EPR10690] ab184568 at a 1/100 dilution. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769), expandable thumbnail](https://content.abcam.com/products/images/lsm5-antibody-epr10690-bsa-and-azide-free-ab250769--western-blot-img81930.jpg "Western blot - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)")
Western blot - Anti-LSM5 antibody [EPR10690] - BSA and Azide free (ab250769)
This data was developed using Anti-LSM5 antibody [EPR10690] ab184568, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-LSM5 antibody [EPR10690] (Anti-LSM5 antibody [EPR10690] ab184568) at 1/10000 dilution
All lanes: T47-D lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 10 kDa
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