Skip to main content

Rabbit Recombinant Monoclonal LSM5 antibody. Suitable for IHC-P, WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-LSM5 antibody [EPR10691(B)] (AB151717), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Mouse
Predicted
Not recommended
Not recommended
Predicted
Not recommended
Rat
Predicted
Not recommended
Not recommended
Predicted
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed:28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed:10523320).

Alternative names

U6 snRNA-associated Sm-like protein LSm5, LSM5

Recommended products

Rabbit Recombinant Monoclonal LSM5 antibody. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10691(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LSM5 also known as U6 snRNA-associated Sm-like protein LSm5 plays an important role in the mechanics of RNA processing. This protein has a mass of approximately 9.7 kDa. LSM5 is expressed in various tissues with notable concentrations found in the cytoplasm and nucleus. It functions as part of the larger Lsm protein family which is known for playing roles in mRNA degradation and splicing.

Biological function summary

LSM5 participates in the formation and stabilization of the U6 snRNA. As a member of the Lsm complex it supports RNA processing and stability. The Lsm complex typically consists of seven Lsm proteins including LSM5 forming a heptameric ring structure. This complex assists in the splicing of pre-mRNA a critical step in the regulation of gene expression. By binding to the 3’ end of U6 snRNA LSM5 facilitates the splicing mechanism necessary for proper mRNA maturation.

Pathways

LSM5 is actively involved in the RNA splicing pathway an important step for gene expression and mRNA maturation. One notable pathway it participates in is the spliceosome cycle closely associated with the processing of pre-mRNA into mature mRNA. In these pathways LSM5 interacts with other components such as U6 snRNP and helps in regulating spliceosomal dynamics. This highlights LSM5's role in maintaining the fidelity and efficiency of mRNA splicing.

Associated diseases and disorders

LSM5 links to certain conditions stemming from defective RNA processing. One example is spinal muscular atrophy a disorder that affects the motor neurons due to impaired splicing. LSM5 also associates with amyotrophic lateral sclerosis (ALS) which involves RNA metabolism disruption. In these diseases LSM5's interaction with proteins like SMN (survival of motor neuron) and TDP-43 can disrupt normal cellular functions and lead to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-LSM5 antibody [EPR10691(B)] (ab151717), expandable thumbnail

    Western blot - Anti-LSM5 antibody [EPR10691(B)] (ab151717)

    All lanes: Western blot - Anti-LSM5 antibody [EPR10691(B)] (ab151717) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: T47D cell lysate at 10 µg

    Lane 3: HL60 cell lysate at 10 µg

    Lane 4: Human placenta lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 10 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717)

    Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling LSM5 with ab151717 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717)

    Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling LSM5 with ab151717 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717)

    Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab151717 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717)

    Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151717 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717)

    Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151717 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (ab151717)

    Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab151717 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com