AB151717

Anti-LSM5 antibody [EPR10691(B)]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal LSM5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

U6 snRNA-associated Sm-like protein LSm5, LSM5

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling LSM5 with ab151717 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling LSM5 with ab151717 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab151717 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab151717 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151717 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151717 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

WB

Unknown

Western blot - Anti-LSM5 antibody [EPR10691(B)] (AB151717)

All lanes:

Western blot - Anti-LSM5 antibody [EPR10691(B)] (ab151717) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

T47D cell lysate at 10 µg

Lane 3:

HL60 cell lysate at 10 µg

Lane 4:

Human placenta lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 10 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10691(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSM5 also known as U6 snRNA-associated Sm-like protein LSm5 plays an important role in the mechanics of RNA processing. This protein has a mass of approximately 9.7 kDa. LSM5 is expressed in various tissues with notable concentrations found in the cytoplasm and nucleus. It functions as part of the larger Lsm protein family which is known for playing roles in mRNA degradation and splicing.

Biological function summary

LSM5 participates in the formation and stabilization of the U6 snRNA. As a member of the Lsm complex it supports RNA processing and stability. The Lsm complex typically consists of seven Lsm proteins including LSM5 forming a heptameric ring structure. This complex assists in the splicing of pre-mRNA a critical step in the regulation of gene expression. By binding to the 3’ end of U6 snRNA LSM5 facilitates the splicing mechanism necessary for proper mRNA maturation.

Pathways

LSM5 is actively involved in the RNA splicing pathway an important step for gene expression and mRNA maturation. One notable pathway it participates in is the spliceosome cycle closely associated with the processing of pre-mRNA into mature mRNA. In these pathways LSM5 interacts with other components such as U6 snRNP and helps in regulating spliceosomal dynamics. This highlights LSM5's role in maintaining the fidelity and efficiency of mRNA splicing.

LSM5 links to certain conditions stemming from defective RNA processing. One example is spinal muscular atrophy a disorder that affects the motor neurons due to impaired splicing. LSM5 also associates with amyotrophic lateral sclerosis (ALS) which involves RNA metabolism disruption. In these diseases LSM5's interaction with proteins like SMN (survival of motor neuron) and TDP-43 can disrupt normal cellular functions and lead to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed : 28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed : 10523320).

See full target information LSM5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:394 PubMed40057671

2025

Single-cell analysis reveals transcriptomic features and therapeutic targets in primary pulmonary lymphoepithelioma-like carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Binghua Tan,Ke Xu,Yingcheng Lyu,Yicheng Liang,Ruihao Liang,Kai Lei,Jialu Liang,Jing Huang,Kefeng Wang,Duoguang Wu,Wenjian Wang,Xueting Hu,Kexi Wang,Minghui Wang,Huayue Lin

World journal of gastrointestinal oncology 16:2716-2726 PubMed38994171

2024

Sm-like 5 knockdown inhibits proliferation and promotes apoptosis of colon cancer cells by upregulating p53, CDKN1A and TNFRSF10B.

Applications

Unspecified application

Species

Unspecified reactive species

Cai-Jing Mo,Xiao-Yuan Deng,Ru-Lan Ma,Kun Zhu,Lei Shi,Kang Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com