AB235332

Anti-LSM7 antibody

Name: Anti-LSM7 antibody (ab235332) | Abcam
Brand: Abcam Limited
SKU: AB235332

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(2 Publications)

Rabbit Polyclonal LSM7 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LSM7.

View Alternative Names

U6 snRNA-associated Sm-like protein LSm7, LSM7

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM7 antibody (AB235332)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM7 antibody (AB235332)

Paraffin-embedded human testis tissue stained for LSM7 with ab235332 (1/100 dilution) in immunohistochemical analysis.

Supplier Data

Western blot - Anti-LSM7 antibody (AB235332)

All lanes:

Western blot - Anti-LSM7 antibody (ab235332) at 1/1000 dilution

All lanes:

Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 12 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LSM7.

Q9UK45

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LSM7 also known as U6 snRNA-associated Sm-like protein LSm7 is a part of the Sm-like family of proteins. This protein has a molecular mass of approximately 9 kDa and is expressed in various tissues with significant presence in the cytoplasm and nucleus. LSM7 predominantly exists as part of the LSm complex that plays an essential role in RNA processing methods.

Biological function summary

LSM7 interacts within the LSm complex which aids in the stabilization and degradation of RNA molecules such as the U6 snRNA. This complex consisting of several proteins ensures the correct splicing of pre-mRNA into mature mRNA which is important for proper gene expression and cellular function. LSM7's binding to U6 snRNA strengthens its involvement in the formation of ribonucleoproteins necessary for RNA splicing.

Pathways

LSM7 participates in the spliceosome assembly and mRNA decay pathways. In the spliceosome LSM7 as part of the LSm complex interacts with proteins such as SmB and SmD ensuring correct splice site recognition and exon joining. In mRNA decay LSM7 facilitates the removal of defective or unnecessary RNA which is significant for maintaining cellular homeostasis by involving proteins like Pat1 and Dcp1.

LSM7's dysfunction has connections to autoimmune diseases such as systemic lupus erythematosus (SLE) as well as neurodegenerative disorders. In SLE antibodies target components of the spliceosome disrupting normal immune function. Additionally its interaction with proteins like U6 snRNA-related Sm proteins highlights its potential role in the development of these conditions emphasizing the importance of LSM7 in maintaining healthy cellular processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed : 28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed : 10523320).

See full target information LSM7

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 10:644 PubMed31501420

2019

CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Liu,Xiao Zhang,Jiafei Lin,Yuxin Chen,Yongxia Qiao,Susu Guo,Yueyue Yang,Guoqing Zhu,Qiuhui Pan,Jiayi Wang,Fenyong Sun

Journal of cellular biochemistry 120:9506-9513 PubMed30520128

2018

Quantitative proteomics using SILAC-MS identifies N-acetylcysteine-solution-triggered reversal response of renal cell carcinoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Cuihong Zhao,Jianhua Chen,Yong Ling,Shenghai Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LSM7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM7 antibody (AB235332)

Western blot - Anti-LSM7 antibody (AB235332)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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