AB134099

Anti-LSP1 antibody [EPR3160]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal LSP1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

WP34, LSP1, Lymphocyte-specific protein 1, 47 kDa actin-binding protein, 52 kDa phosphoprotein, Lymphocyte-specific antigen WP34, pp52

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab134099 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

Immunohistochemical analysis of paraffin embedded normal Human colon lymphocytes tissue using ab134099 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab134099 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

Immunohistochemical analysis of paraffin embedded normal Human breast lymphocytes tissue using ab134099 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab134099 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR3160] (AB134099)

Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab134099 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-LSP1 antibody [EPR3160] (AB134099)

WB

Unknown

Western blot - Anti-LSP1 antibody [EPR3160] (AB134099)

All lanes:

Western blot - Anti-LSP1 antibody [EPR3160] (ab134099) at 1/1000 dilution

Lane 1:

Daudi cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

HuT-7 cell lysate at 10 µg

Lane 4:

U937 cell lysate at 10 µg

Lane 5:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

false

Carrier free

Anti-LSP1 antibody [EPR3160] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3160

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lymphocyte-specific protein 1 also known as LSP1 is a 52 kDa intracellular F-actin binding protein. It plays a role in cytoskeletal organization and cell motility. This protein has significant expression in lymphocytes neutrophils macrophages and endothelial cells. LSP1 modulates several cellular functions including adhesion and migration by interacting with the actin cytoskeleton. Known alternatively as pp52 and WD-40 repeat-containing protein 1 LSP1 acts as a link connecting signal transduction from cell surface receptors to cytoskeletal regions.

Biological function summary

LSP1 influences immune cell regulation and inflammatory response. By acting as an important player in the modulation of actin polymerization it contributes to leukocyte chemotaxis and transmigration. LSP1 forms part of a larger protein complex involved in reorganizing the cytoskeleton facilitating cellular dynamics necessary for immune response. Additionally interactions with adapter proteins like 14-3-3 add another level of functional regulation.

Pathways

LSP1 integrates into the chemokine signaling pathway and the P38 MAPK signaling pathway. In these pathways it exerts influence on cellular processes such as migration and response to external stimuli. It shows connections with proteins such as CXCR4 and MAPK highlighting its role in conveying chemotactic signals that direct immune cells toward inflammatory sites. These interactions are integral in establishing directionality and speed of cell movement in response to chemokine gradients.

Aberrations in LSP1 function or expression link to autoimmune conditions like systemic lupus erythematosus and vascular disorders like atherosclerosis. Its role in mediating vascular permeability and leukocyte migration suggests that dysregulation can contribute to pathogenic inflammation. Related proteins like MCP-1 and ICAM-1 participate in these disease pathways highlighting the involvement of LSP1 in modulating endothelial responses and immune cell recruitment at sites of inflammation.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May play a role in mediating neutrophil activation and chemotaxis.

See full target information LSP1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:11295 PubMed40175394

2025

Identification of novel IL17-related genes as prognostic and therapeutic biomarkers of psoriasis using comprehensive bioinformatics analysis and machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Xingling He,Jingjing Huang,Hanying Ma,Zhujun Ma,Changzheng Huang,Yunli Ling,Bin Zhou,Jingang Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com